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ENPP3

外核苷酸焦磷酸酶/磷酸二酯酶家族成員3是一種由ENPP3基因編碼的酶。Hiroaki在2013年的實(shí)驗(yàn)中對N2a細(xì)胞進(jìn)行研究,純化了存在于GnT-IX中的一種內(nèi)源性抑制因子,發(fā)現(xiàn)其與外核苷酸焦磷酸酶/磷酸二酯酶3 (ENPP3)相同,這一結(jié)果通過質(zhì)譜分析以及培養(yǎng)細(xì)胞中ENPP3的敲除和過表達(dá)得到了證明。動(dòng)力學(xué)分析顯示,ENPP3導(dǎo)致的GnT-IX抑制機(jī)制是ENPP3介導(dǎo)的核苷酸糖供體底物UDP-GlcNAc水解,從而生成UMP(一種強(qiáng)效競爭性GnT-IX抑制劑)。實(shí)際上,當(dāng)對細(xì)胞內(nèi)的ENPP3敲除,細(xì)胞內(nèi)核苷酸糖水平顯著升高,并顯示出細(xì)胞總糖基化特征的變化。除了糖基轉(zhuǎn)移酶的伴侶或其他已知調(diào)節(jié)劑外,ENPP3介導(dǎo)的核苷酸糖水解將對糖基轉(zhuǎn)移酶活性產(chǎn)生也會(huì)產(chǎn)生廣泛和顯著的影響,并負(fù)責(zé)改變細(xì)胞總糖基化特征和調(diào)節(jié)細(xì)胞功能。

熱銷產(chǎn)品

Recombinant Macaca fascicularis Ectonucleotide pyrophosphatase/phosphodiesterase 3(ENPP3),partial (Active) (CSB-MP4278MOV)

驗(yàn)證數(shù)據(jù)

SDS

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

ELISA

Activity: Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis ENPP3 at 2 μg/ml can bind Anti-ENPP3 recombinant antibody (CSB-RA007681MA1HU), the EC50 is 3.313-4.724 ng/mL.

Recombinant Human Ectonucleotide pyrophosphatase/phosphodiesterase family member 3(ENPP3),partial (Active)(CSB-MP007681HU)

驗(yàn)證數(shù)據(jù)

SDS

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

ELISA

Activity: Measured by its binding ability in a functional ELISA. Immobilized Human ENPP3 at 2 μg/ml can bind anti-ENPP3 recombinant antibody (CSB-RA007681MA1HU), the EC50 is 2.151-2.492 ng/mL.

ENPP3 Recombinant Monoclonal Antibody CSB-RA007681MA1HU

驗(yàn)證數(shù)據(jù)

ELISA
CSB-RA007681MA1HU ELISA

The Binding Activity of Macaca fascicularis ENPP3 with Anti-ENPP3 recombinant antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis ENPP3 (CSB-MP4278MOV) at 2 μg/mL can bind Anti-ENPP3 recombinant antibody, the EC50 is 3.313-4.724 ng/mL.

ELISA
CSB-RA007681MA1HU ELISA

The Binding Activity of Huamn ENPP3 with Anti- ENPP3 Recombinant Antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human ENPP3(CSB-MP007681HU) at 2 μg/mL can bind Anti-ENPP3 recombinant antibody, the EC50 is 2.151-2.492 ng/mL

FC
CSB-RA007681MA1HU FC

Untransfected HEK293T cells (green line) and transfected Human ENPP3 HEK293T stable cells (red line) were stained with anti-ENPP3 recombinant antibody (2μg/1*106cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.

HEK293T/Human ENPP3 Stable Cell Line CSB-SC007681HU

驗(yàn)證數(shù)據(jù)

FC
CSB-SC007681HU FC

Untransfected HEK293T cells (green line) and transfected Human ENPP3 HEK293T Stable cells (red line) were stained with anti-ENPP3 antibody (2μg/1*106cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.

ENPP3 Antibodies

ENPP3 for Homo sapiens (Human)

ENPP3 Proteins

ENPP3 Proteins for Rattus norvegicus (Rat)

ENPP3 Proteins for Bos taurus (Bovine)

ENPP3 Proteins for Homo sapiens (Human)

ENPP3 Proteins for Pongo abelii (Sumatran orangutan)

ENPP3 Proteins for Mus musculus (Mouse)

ENPP3 ELISA Kit

ENPP3 ELISA Kit for Homo sapiens (Human)



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