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HEK293T/Human ENPP3 Stable Cell Line

  • 貨號(hào):
    CSB-SC007681HU
  • 規(guī)格:
    1 vial contains approximately 5x106 cells in 1 ml
  • 價(jià)格:
    ¥30000
  • 活性:
    • Untransfected HEK293T cells (green line) and transfected Human ENPP3 HEK293T Stable cells (red line) were stained with anti-ENPP3 antibody (2μg/1*106cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.
  • 其他:

產(chǎn)品詳情

  • Uniprot No.:
  • 生長(zhǎng)類型:
    Adherent
  • 抗性:
    Puromycin
  • 培養(yǎng)基:
    DMEM + 10% FBS + 2μg/mL Puromycin
  • 別名:
    ENPP3
  • 蛋白標(biāo)簽:
    Tag-Free
  • 保存緩沖液:
    35% FBS + 55% DMEM + 10% DMSO
  • 應(yīng)用范圍:
    Binding assay by FACS and cell based ELISA.
  • 儲(chǔ)存條件:
    Frozen in liquid nitrogen or stored at -80°C
  • 貨期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

靶點(diǎn)詳情

  • 功能:
    Hydrolase that metabolizes extracellular nucleotides, including ATP, GTP, UTP and CTP. Limits mast cell and basophil responses during inflammation and during the chronic phases of allergic responses by eliminating the extracellular ATP that functions as signaling molecule and activates basophils and mast cells and induces the release of inflammatory cytokines. Metabolizes extracellular ATP in the lumen of the small intestine, and thereby prevents ATP-induced apoptosis of intestinal plasmacytoid dendritic cells. Has also alkaline phosphodiesterase activity.
  • 基因功能參考文獻(xiàn):
    1. We showed the ability of the immunological marker CD203c to predict the clinical efficacy of sublingual immunotherapy on rhinitis subjects allergic to Parietaria. PMID: 29702283
    2. Basophil CD203c surface expression reliably discriminated cystic fibrosis with allergic bronchopulmonary aspergillosis from cystic fibrosis with Aspergillus colonization and cystic fibrosis over time. PMID: 26585435
    3. Expression of CD203c on basophils as a marker of immunoglobulin E-mediated (L)-asparaginase allergy. PMID: 23581640
    4. Anaphylactic transfusion reaction in homozygous haptoglobin deficiency detected by CD203c expression on basophils. PMID: 24497482
    5. ENPP3 is a regulator of N-acetylglucosaminyltransferase GnT-IX (GnT-Vb) PMID: 23960081
    6. The early signaling requirements for the CD11b/CD203c compartment expression and CD63 degranulation provide support for the hypothesis that CD11b and CD203c reside in a similar compartment. PMID: 22722613
    7. Subjects with nut allergy show an increase of basophil CD203c levels at baseline and following rapid ex vivo stimulation with nut allergen PMID: 20975283
    8. Asthma exacerbation was accompanied by increased expression of CD203c on basophils that decreased significantly during remission PMID: 20159259
    9. Influence of hyperosmotic conditions on basophil CD203c upregulation in patients with food-dependent exercise-induced anaphylaxis. PMID: 20047266
    10. Data show that low and high dilutions of histamine inhibit CD203c up-regulation in anti-IgE stimulated basophils. PMID: 19418203
    11. E-NPP3 is associated with carcinogenesis of human colon cancer and that serum E-NPP3 might be a tumor marker of colon carcinoma PMID: 14533006
    12. E-NPP3 is involved in the infiltration of neoplastic bile duct carcinoma PMID: 15072822
    13. Our data demonstrate that leptin promotes platelet activation, provides a mechanistic basis for the prothrombotic effect of this hormone, and identifies a potentially novel therapeutic avenue to limit obesity-associated cardiovascular disease. PMID: 15886225
    14. In conclusion, using well-defined experimental conditions, the measurement of CD203c up-regulation on basophils in response to specific allergens is as reliable as CD63-BAT for the in vitro diagnosis of patients with IgE-mediated allergy. PMID: 17275019

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  • 亞細(xì)胞定位:
    Cell membrane; Single-pass type II membrane protein. Apical cell membrane; Single-pass type II membrane protein. Secreted.
  • 蛋白家族:
    Nucleotide pyrophosphatase/phosphodiesterase family
  • 組織特異性:
    Detected on bile ducts in liver, and in blood serum (at protein level). Detected in prostate and uterus. Detected on basophils, but not neutrophils.
  • 數(shù)據(jù)庫(kù)鏈接:

    HGNC: 3358

    OMIM: 602182

    KEGG: hsa:5169

    STRING: 9606.ENSP00000350265

    UniGene: Hs.486489



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