Recombinant Mouse Nischarin (Nisch), partial

中文名稱：

小鼠Nisch重組蛋白
貨號：

CSB-EP015821MO
規格：

￥1836
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
生物活性：

Not Test
基因名：

Nisch
Uniprot No.：

Q80TM9
別名：

Imidazoline receptor 1;I-1;IR1;Imidazoline receptor I-1-like protein;Imidazoline-1 receptor;I1R
種屬：

Mus musculus (Mouse)
蛋白長度：

Partial
來源：

E.coli
分子量：

47.4
表達區域：

1241-1593aa
氨基酸序列

EVENEELRHLLWSSVVFYQTPGLEVTACVLLSSKAVYFILHDGLRRYFSEPLQDFWHQKNTDYNNSPFHVSQCFVLKLSDLQSVNVGLFDQYFRLTGSSPTQVVTCLTRDSYLTHCFLQHLMLVLSSLERTPSPEPVDKDFYSEFGDKNTGKMENYELIHSSRVKFTYPSEEEVGDLTYIVAQKMADPAKNPALSILLYIQAFQVVTPHLGRGRGPLRPKTLLLTSAEIFLLDEDYIHYPLPEFAKEPPQRDRYRLDDGRRVRDLDRVLMGYYPYPQALTLVFDDTQGHDLMGSVTLDHFGEMPGGPGRVGQGREVQWQVFVPSAESREKLISLLARQWEALCGRELPVELTG
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

C-terminal 6xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Acts either as the functional imidazoline-1 receptor (I1R) candidate or as a membrane-associated mediator of the I1R signaling. Binds numerous imidazoline ligands that induces initiation of cell-signaling cascades triggering to cell survival, growth and migration. Its activation by the agonist rilmenidine induces an increase in phosphorylation of mitogen-activated protein kinases MAPK1 and MAPK3 in rostral ventrolateral medulla (RVLM) neurons that exhibited rilmenidine-evoked hypotension. Blocking its activation with efaroxan abolished rilmenidine-induced mitogen-activated protein kinase phosphorylation in RVLM neurons. Acts as a modulator of Rac-regulated signal transduction pathways. Suppresses Rac1-stimulated cell migration by interacting with PAK1 and inhibiting its kinase activity. Also blocks Pak-independent Rac signaling by interacting with RAC1 and inhibiting Rac1-stimulated NF-kB response element and cyclin D1 promoter activation. Inhibits also LIMK1 kinase activity by reducing LIMK1 'Tyr-508' phosphorylation. Inhibits Rac-induced cell migration and invasion in breast and colon epithelial cells. Inhibits lamellipodia formation, when overexpressed. Plays a role in protection against apoptosis. Involved in association with IRS4 in the enhancement of insulin activation of MAPK1 and MAPK3. When overexpressed, induces a redistribution of cell surface ITGA5 integrin to intracellular endosomal structures.
基因功能參考文獻：
1. results indicate that Nisch is an important AMPK inhibitor and a critical regulator of energy homeostasis, including lipid and glucose metabolism. PMID: 28842496
2. We identified a significant genetic interaction between Nisch and Itga5; mice heterozygous for Itga5-null and homozygous for edison mutations display a significantly increased penetrance and severity of chronic otitis media. PMID: 28806779
3. Nischarin inhibits neurite outgrowth by blocking PAK1 activation in neurons. PMID: 26670864
4. Data from studies using various receptor antagonists/agonists suggest that imidazoline receptors (Nisch I-1), alpha2-adrenoceptors, and endothelin-A receptors are involved in body temperature regulation in mice. PMID: 22681550
5. Activation of I1-imidazoline receptor ameliorated energy intake and epididymal white adipose tissue accumulation. PMID: 23516130
6. Data suggest that both IR1 and IR2 (imidazoline receptor 2) play roles in acquisition of behavioral responses in alcoholism; activation of IR1 and IR2 may serve as molecular targets in prevention (and possibly treatment) of alcoholism. PMID: 23399882
7. Activation of cerebral I(1)-imidazoline receptor decreased hyperphagia in streptozotocin-induced diabetic mice. PMID: 22674473
8. Activation of I-1 R may activate FXR to lower plasma lipids, suggesting I-1 R as a new target for the treatment of hyperlipidemia. PMID: 21484668
9. Report imidazoline I(1) receptor-mediated reduction of muscle rigidity in the reserpine-treated murine model of Parkinson's disease. PMID: 18602099
10. guanidine increases glucose uptake via an activation of imidazoline I2 receptor through AMPK activation in skeletal muscle cell. PMID: 20298750
11. Nisch regulates cell movement by inhibiting PAK. PMID: 15229651
12. Data suggest that nischarin, in addition to regulating the p21-activated kinase (PAK) strand of Rac1 signaling, can also regulate other links in the web of Rac1 signaling pathways. PMID: 16002401
13. Nischarin may serve as the functional I1-receptor PMID: 16678176
顯示更多

收起更多
亞細胞定位：

Cell membrane. Cytoplasm. Early endosome. Recycling endosome.
組織特異性：

Highly expressed in brain and kidney. Moderately expressed in heart, liver, lung and skeletal muscle. Not detected in spleen and testis.
數據庫鏈接：

KEGG: mmu:64652

STRING: 10090.ENSMUSP00000022469

UniGene: Mm.298728