Recombinant Human Serine racemase (SRR)

^{In Stock}

貨號：

CSB-EP022691HUc0
規格：
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

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靶點詳情

產品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
基因名：

SRR
Uniprot No.：

Q9GZT4
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length
來源：

E.coli
分子量：

68.1 kDa
表達區域：

1-340aa
氨基酸序列

MCAQYCISFADVEKAHINIRDSIHLTPVLTSSILNQLTGRNLFFKCELFQKTGSFKIRGALNAVRSLVPDALERKPKAVVTHSSGNHGQALTYAAKLEGIPAYIVVPQTAPDCKKLAIQAYGASIVYCEPSDESRENVAKRVTEETEGIMVHPNQEPAVIAGQGTIALEVLNQVPLVDALVVPVGGGGMLAGIAITVKALKPSVKVYAAEPSNADDCYQSKLKGKLMPNLYPPETIADGVKSSIGLNTWPIIRDLVDDIFTVTEDEIKCATQLVWERMKLLIEPTAGVGVAAVLSQHFQTVSPEVKNICIVLSGGNVDLTSSITWVKQAERPASYQSVSV
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-GST-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Catalyzes the synthesis of D-serine from L-serine. D-serine is a key coagonist with glutamate at NMDA receptors. Has dehydratase activity towards both L-serine and D-serine.
基因功能參考文獻：
1. SRR was identified as a type 2 diabetes susceptibility gene. SRR plays a role in insulin secretion in vitro. PMID: 28580277
2. rs391300 SNP, located on the serine racemase (SRR) gene and linked to increased susceptibility to type 2 diabetes, was associated with progression from mild cognitive impairment to probable Alzheimer's disease. PMID: 29338921
3. Study found an inverse association between the genetic risk off schizophrenia based on 108 genome-wide significantly associated SNPs and the prevalence for treated migraine in a general population sample. This association was primary linked to SNPs associated with genes encoding proteins involved in glutamatergic neurotransmission and could be attributed to the single intronic variant rs4523957 in SRR. PMID: 27394076
4. Data suggest that Ser-84 and Arg-135 are important in catalysis and substrate specificity of SRR. PMID: 28696262
5. Magnesium and calcium ions differentially affect human serine racemase activity and modulate its quaternary equilibrium toward a tetrameric form PMID: 28089597
6. MiR-193a-3p and miR-193a-5p play important roles in osteosarcoma metastasis through down-regulation of the Rab27B and SRR genes and therefore may serve as useful biomarkers for the diagnosis of osteosarcoma PMID: 26913720
7. Loss-of-function mutation of the gene encoding serine racemase significantly attenuates excitotoxicity in retina. PMID: 26485193
8. Serine racemase activity and dynamics are regulated by halides, ATP and malonate. PMID: 25331425
9. In serine racemase, similarly to the related enzyme alanine racemase, the unprotonated pyridoxal-5'-phosphate -substrate intermediate is stabilized mostly due to solvation effects contributed by water molecules and active-site residues. PMID: 25493718
10. FBXO22 protein is required for optimal synthesis of NMDA receptor coagonist D-serine by interacting with serine racemase, activating it, and preventing its targeting to membranes. PMID: 25336657
11. cross-talk between allosteric and active sites, leading to the stabilization of two alternative protein conformations with ATP affinities of ~ 10 muM and 1.8 mm PMID: 23992455
12. S84A serine racemase mutant behaved like serine dehydratase, whereas A65S serine dehydratase mutant acquired an additional function of using D-serine as a substrate. PMID: 23112234
13. The structural characteristics of SR obtained from live cells suggest that SR is sensitive to oxidation in vivo, perhaps consistent with a scenario in which such modification plays a role in feedback or other forms of regulation. PMID: 22151352
14. Serine racemase and D-serine are involved in both pre-symptomatic and progressive phases of amyotrophic lateral sclerosis, demonstrating a link between mutant superoxide dismutase (SOD)1 and a glial-derived toxic mediator in transgenic mice. PMID: 22117694
15. The SRR mRNA is elevated in people death with suicide. PMID: 20385472
16. The structure of mammalian serine racemase: evidence for conformational changes upon inhibitor binding PMID: 20106978
17. Data report on the isolation of a cDNA encoding a human serine racemase (SRR) from a human neuronal like cell line. PMID: 15193426
18. D-serine is synthesized in human placenta by the racemization of L-serine by serine racemase. PMID: 15219883
19. serine racemase catalyzes the degradation of cellular D-serine itself, through the alpha,beta-elimination of water PMID: 15536068
20. The frequency of the genotypes showed that 5'-G/C serine racemase is not a major risk factor for schizophrenia. PMID: 16446740
21. Expression of serine racemaseusing Western blot analysis in postmortem hippocampus and cortex in schizophrenia and a comparison group. PMID: 16837850
22. Not associated with schizophrenia in a Gefman case-control study. PMID: 17413455
23. Not associated with bipolar disorder in a German case-control study. PMID: 17413456
24. observed activation of serine racemase by divalent cations has been assumed to be a side-effect associated with ATP binding, which is known to form a complex with Mg(2+) ions PMID: 17697119
25. serine racemase and D-amino acid oxidase are expressed in human brain and demonstrate aberrant D-serine metabolism in schizophrenia PMID: 17880399
26. Analysis of SRR genetic variants in humans identified a robust association with schizophrenia. PMID: 19483194
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蛋白家族：

Serine/threonine dehydratase family
組織特異性：

Brain: expressed at high levels in hippocampus and corpus callosum, intermediate levels in substantia nigra and caudate, and low levels in amygdala, thalamus, and subthalamic nuclei. Expressed in heart, skeletal muscle, kidney and liver.
數據庫鏈接：

HGNC: 14398

OMIM: 606477

KEGG: hsa:63826

STRING: 9606.ENSP00000339435

UniGene: Hs.461954