Recombinant Human Trophoblast glycoprotein (TPBG), partial (Active)

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評價 (1)

中文名稱：

人TPBG重組蛋白
貨號：

CSB-MP024093HUb0
規格：

￥816
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized Human TPBG at 2 μg/mL can bind Anti-TPBG recombinant antibody (CSB-RA024093MA1HU), the EC₅₀ is 1.230-1.519 ng/mL.
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靶點詳情

產品詳情

純度：

Greater than 95% as determined by SDS-PAGE.
內毒素：

Less than 1.0 EU/ug as determined by LAL method.
生物活性：

Measured by its binding ability in a functional ELISA. Immobilized Human TPBG at 2 μg/mL can bind Anti-TPBG recombinant antibody (CSB-RA024093MA1HU), the EC50 is 1.230-1.519 ng/mL.
基因名：

TPBG
Uniprot No.：

Q13641
別名：

(5T4 oncofetal antigen)(5T4 oncofetal trophoblast glycoprotein)(5T4 oncotrophoblast glycoprotein)(M6P1)(Wnt-activated inhibitory factor 1)(WAIF1)
分子結構：
種屬：

Homo sapiens (Human)
蛋白長度：

Partial
來源：

Mammalian cell
分子量：

38.9 kDa
表達區域：

32-355aa
氨基酸序列

SSPTSSASSFSSSAPFLASAVSAQPPLPDQCPALCECSEAARTVKCVNRNLTEVPTDLPAYVRNLFLTGNQLAVLPAGAFARRPPLAELAALNLSGSRLDEVRAGAFEHLPSLRQLDLSHNPLADLSPFAFSGSNASVSAPSPLVELILNHIVPPEDERQNRSFEGMVVAALLAGRALQGLRRLELASNHFLYLPRDVLAQLPSLRHLDLSNNSLVSLTYVSFRNLTHLESLHLEDNALKVLHNGTLAELQGLPHIRVFLDNNPWVCDCHMADMVTWLKETEVVQGKDRLTCAYPEKMRNRVLLELNSADLDCDPILPPSLQTS
蛋白標簽：

N-terminal?10xHis-tagged
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
產品描述：

This Human TPBG recombinant protein was produced in mammalian cell, where the gene sequence encoding Human TPBG (32-355aa) was expressed with the N-terminal 10xHis tag. The purity of this TPBG protein was greater than 95%. The activity was validated.

TPBG was first discovered in embryonic development. It is highly expressed in placental trophoblast cells and is basically absent in normal tissues. It only exists in some special epithelia, such as basal stratified squamous epithelium, glandular and ductal epithelium, and retina. Secondary neurons and olfactory bulbs. Later, it was found that TPBG is expressed in many cancers, including uterine cancer, ovarian cancer, colorectal cancer, gastric cancer, kidney cancer, prostate cancer and so on. In colon, gastric, or ovarian cancer, there is evidence that the expression of TPBG is associated with low cancer cure rates. In the tissues of non-small cell lung cancer, kidney cancer or pancreatic cancer, the expression of TPBG is as high as more than 95%. Overexpression of TPBG in tumors is associated with disease progression. It may play a role in affecting the connections between tumor cells, affecting cell morphology, motility and adhesion, and then promoting distant metastasis.
Datasheet & COA：

Please contact us to get it.

產品評價

平均分:

5.0分 - 1 個評價

實驗應用：抗原

產品評價：多次購買CUSABIO的產品，相比其他廠家，蛋白活性好，性能穩定，很大程度上可以保證實驗的成功率。采購方便，貨期也很快！

By 章老師

靶點詳情

功能：

May function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization.
基因功能參考文獻：
1. In extravillous trophoblasts, 5T4 may function in epithelial-to-mesenchymal transition during placentation. The role of syncytiotrophoblast 5T4 is unknown, but its abundance in shed syncytial vesicles may signify route of sensitization of the maternal immune system. PMID: 28481180
2. Collectively, these findings demonstrate that an anti-5T4 antibody-drug conjugate reduces the fraction of cancer stem cells (CSC), and prevents local recurrence and suggest a novel therapeutic approach for patients with head and neck squamous cell carcinoma. PMID: 27780858
3. We obtained GWS evidence (P < 2.7 x 10(-6)) for gene-based association in the total sample with a novel locus, TPBG (P = 1.8 x 10(-6)). PMID: 28183528
4. In conclusion, the present study provided evidence that TPBG is involved in PDAC metastasis, and that TPBG and its associated signaling pathways may be a suitable target for pancreatic ductal adenocarcinoma therapy. PMID: 25738465
5. 5T4 expression is mechanistically associated with the directional movement of cells through epithelial mesenchymal transition, facilitation of CXCL12/CXCR4 chemotaxis, blocking of canonical Wnt/beta-catenin while favouring non-canonical pathway signalling PMID: 25066861
6. Tyrosine 325 plus the leucine-rich repeat 1 surface centered on a second exposed aromatic residue, phenylalanine 97, are essential for inhibition of Wnt/beta-catenin signaling. PMID: 24582434
7. The surface expression of 5T4 marks the use of the CXCR4 rather than the CXCR7 receptor, with distinct consequences for CXCL12 exposure. PMID: 22956548
8. 5T4 is a potential new antigen for targeted therapies such as immunotherapy in MPM, as it is overexpressed on mesothelioma cells and recognised by 5T4-specific cytotoxic T-cells. PMID: 22498111
9. Glycosylation and epitope mapping of the 5T4 glycoprotein oncofoetal antigen PMID: 11903056
10. Results sugest that 5T4 is a transient marker of human embryonic stem cell differentiation & that 5T4 phenotype, colony seeding density and culture conditions influence the maintenance of pluripotent hES cells and their differentiation to neural lineages. PMID: 16616918
11. there is a repertoire of CD8 T cell recognition of 5T4 in normal human donors and some candidate HLA-A*0201 epitopes have been identified PMID: 16646078
12. the mRNA expression of trophoblast glycoprotein is up-regulated in cells circulating within blood from women with preeclampsia. PMID: 17978129
13. 5T4-based cancer vaccine: cytotoxic T lymphocyte epitopes identified in Trovax-vaccinated colorectal cancer patients PMID: 18567615
14. colorectal cancer patients who had preexisting cell proliferative responses to 5T4 were longer-term survivors PMID: 18833005
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亞細胞定位：

Cell membrane; Single-pass type I membrane protein.
組織特異性：

Expressed by all types of trophoblasts as early as 9 weeks of development. Specific for trophoblastic cells except for amniotic epithelium. In adult tissues, the expression is limited to a few epithelial cell types but is found on a variety of carcinoma.
數據庫鏈接：

HGNC: 12004

OMIM: 190920

KEGG: hsa:7162

STRING: 9606.ENSP00000358765

UniGene: Hs.71947