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  4. Recombinant Arabidopsis thaliana ATP-dependent DNA helicase Q-like 2 (RECQL2), partial

Recombinant Arabidopsis thaliana ATP-dependent DNA helicase Q-like 2 (RECQL2), partial

  • 中文名稱：
    擬南芥MED34重組蛋白
  • 貨號：
    CSB-YP880823DOA
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    擬南芥MED34重組蛋白
  • 貨號：
    CSB-EP880823DOA
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    擬南芥MED34重組蛋白
  • 貨號：
    CSB-EP880823DOA-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    擬南芥MED34重組蛋白
  • 貨號：
    CSB-BP880823DOA
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    擬南芥MED34重組蛋白
  • 貨號：
    CSB-MP880823DOA
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    MED34
  • Uniprot No.：
  • 別名：
    RECQL2; MED34; MED34_1; RECQ2; RQL2; At1g31360; T19E23.16; ATP-dependent DNA helicase Q-like 2; EC 3.6.4.12; RecQ-like protein 2; AtRQ2; AtRecQ2; AtRecQl2
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    3'-5' DNA helicase that may play a role in the repair of DNA. Its DNA unwinding activity in vitro is dependent on magnesium, and ATP or dATP. Can use GTP/dGTP, CTP/dCTP or UTP/dUTP as nucleotide cofactors. Catalyzes Holliday junction branch migration and replication fork regression. Disrupts D-loop structures.
  • 基因功能參考文獻：
    1. RecQ helicases AtRECQ2 and AtRECQ3 both show highly repetitive DNA unwinding. Although AtRECQ2 preferentially orients itself towards the junction to unwind it, AtRECQ3 moves in the opposite direction and processively rewinds, that is, helps closing, unzipped hairpins over hundreds of bases. Thus RecQ helicases can exhibit antagonistic activities on the molecular level. [RECQ2] PMID: 23771268
    2. AtRECQ2 is a (d)NTP-dependent 3'-->5' DNA helicase. AtRECQ2 is able to disrupt recombinogenic structures by disrupting various D-loop structures PMID: 18419780
  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    Helicase family, RecQ subfamily
  • 組織特異性：
    Expressed in shoots and flowers. Expressed in young leaves, inflorescences, roots, shoot apical meristem, young siliques, and mature green siliques.
  • 數據庫鏈接：

    KEGG: ath:AT1G31360

    STRING: 3702.AT1G31360.1

    UniGene: At.40359



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