Recombinant Mouse Semaphorin-4D (Sema4d), partial (Active)

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中文名稱：

小鼠Sema4d重組蛋白
貨號：

CSB-MP020990MO
規格：

￥696
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized Pepinemab at 2 μg/ml can bind Mouse Sema4d, the EC₅₀ is 4.878-7.446 ng/ml. Biological Activity Assay
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靶點詳情

產品詳情

純度：

Greater than 95% as determined by SDS-PAGE.
內毒素：

Less than 1.0 EU/ug as determined by LAL method.
生物活性：

Measured by its binding ability in a functional ELISA. Immobilized Pepinemab at 2 μg/ml can bind Mouse Sema4d, the EC₅₀ is 4.878-7.446 ng/ml.
基因名：

Sema4d
Uniprot No.：

O09126
別名：

(M-Sema G)(Semaphorin-C-like 2)(Semaphorin-J)(Sema J)(CD100)
分子結構：
種屬：

Mus musculus (Mouse)
蛋白長度：

Partial
來源：

Mammalian cell
分子量：

108.1 kDa
表達區域：

24-733aa
氨基酸序列

FAPVPRLTWEHGEVGLVQFHKPGIFNYSALLMSEDKDTLYVGAREAVFAVNALNISEKQHEVYWKVSEDKKSKCAEKGKSKQTECLNYIRVLQPLSSTSLYVCGTNAFQPTCDHLNLTSFKFLGKSEDGKGRCPFDPAHSYTSVMVGGELYSGTSYNFLGSEPIISRNSSHSPLRTEYAIPWLNEPSFVFADVIQKSPDGPEGEDDKVYFFFTEVSVEYEFVFKLMIPRVARVCKGDQGGLRTLQKKWTSFLKARLICSKPDSGLVFNILQDVFVLRAPGLKEPVFYAVFTPQLNNVGLSAVCAYTLATVEAVFSRGKYMQSATVEQSHTKWVRYNGPVPTPRPGACIDSEARAANYTSSLNLPDKTLQFVKDHPLMDDSVTPIDNRPKLIKKDVNYTQIVVDRTQALDGTFYDVMFISTDRGALHKAVILTKEVHVIEETQLFRDSEPVLTLLLSSKKGRKFVYAGSNSGVVQAPLAFCEKHGSCEDCVLARDPYCAWSPAIKACVTLHQEEASSRGWIQDMSGDTSSCLDKSKESFNQHFFKHGGTAELKCFQKSNLARVVWKFQNGELKAASPKYGFVGRKHLLIFNLSDGDSGVYQCLSEERVRNKTVSQLLAKHVLEVKMVPRTPPSPTSEDAQTEGSKITSKMPVASTQGSSPPTPALWATSPRAATLPPKSSSGTSCEPKMVINTVPQLHSEKTVYLKSSDNR
蛋白標簽：

C-terminal mFc-tagged
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
產品描述：

The Sema4d was produced as a fusion protein with a C-terminal mFc-tag in mammalian cells. Its expression region corresponds to the amino acid 24-733 of the mouse Sema4d protein. This recombinant Sema4d protein is an active protein and its activity has been validated through the ELISA. In the functional ELISA, this mouse Sema4d protein can bind to the Pepinemab, with the EC₅₀ of 4.878-7.446 ng/ml. The purity of this Sema4d protein is greater than 95% measured by SDS-PAGE. On the gel, this Sema4d protein migrated to the molecular weight band of about 117 kDa. It contains less endotoxin, <1.0 EU/ug determined by the LAL method. And it is available now.

Sema4d is a transmembrane homodimer involved in the inactivation of platelets, stimulation of angiogenesis, and regulation of bone formation. It also possesses immune regulatory activity. Studies have shown that Sema4d is hyper-expressed in some malignancies and facilitates tumor growth and angiogenesis.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Cell surface receptor for PLXNB1 and PLXNB2 that plays an important role in cell-cell signaling. Regulates GABAergic synapse development. Promotes the development of inhibitory synapses in a PLXNB1-dependent manner. Modulates the complexity and arborization of developing neurites in hippocampal neurons by activating PLXNB1 and interaction with PLXNB1 mediates activation of RHOA. Promotes the migration of cerebellar granule cells. Plays a role in the immune system; induces B-cells to aggregate and improves their viability (in vitro). Induces endothelial cell migration through the activation of PTK2B/PYK2, SRC, and the phosphatidylinositol 3-kinase-AKT pathway.
基因功能參考文獻：
1. These results reveal a novel signaling network, the Sema4D-RhoA-Akt signal cascade, that coordinates cellular function and morphology and highlights the importance of specific spatiotemporally restricted components of a signaling pathway in the regulation of ameloblast differentiation. PMID: 27218883
2. our findings revealed for the first time that Sema4D positively regulated both the adaptive and innate immune responses in contact hypersensitivity PMID: 27614265
3. SEMA4D-/- neutrophils show impaired NET formation when in contact with endothelial cells. PMID: 28416516
4. Absence of Sema4D improves oligodendrocyte recovery after cerebral ischemia/reperfusion injury in mice PMID: 26752319
5. Sema4D-deficiency affected ischemia-induced microglial activation in morphology, iNOS expression, and proliferation, and this deficiency most likely resulted in moderation of cytotoxicity and locomotor behavioral abnormality after cerebral ischemia PMID: 26202989
6. The addition of recombinant Sema4D to Sema4D/ vaginal epithelial cells in culture significantly enhanced apoptosis of the vaginal epithelial cells. PMID: 25351707
7. Sema4D may be an essential apoptosis-inducing ligand that acts downstream of estrogen action in vaginal epithelium during pubertal tissue remodeling. PMID: 24841081
8. therapeutic effects via bone-targeting systems featuring interference of Sema4d are able to partly counteract alveolar bone loss caused by osteoporosis. PMID: 25252878
9. CD100-mediated signals are critical for effective activation of gammadelta IEL to produce growth factors, including KGF-1, that are required for healing of the colon epithelium during colitis. PMID: 23695512
10. Deletion of plexin-B1, the high affinity receptor for semaphorin 4D, significantly (one-way ANOVA; p < 0.001) reduced thrombus weight (- 40%) in oxidative venous thrombosis. PMID: 23642655
11. Data indicate neuroimmune semaphorin 4D (Sema4D) as an important regulator of Th2-driven lung pathophysiology and as a potential target for a combinatory disease immunotherapy. PMID: 23911404
12. Sema 4D/CD100 is not only a ligand for plexin B1 but also a direct signaling receptor in mediating Sema 4D/CD100-plexin B's biological functions. PMID: 23262975
13. CD100 deficiency in vivo resulted in delayed repair of cutaneous wounds due to a disrupted gamma-delta T cell response to keratinocyte damage. PMID: 22902232
14. Sema4D may act as an intrinsic inhibitory regulator of oligodendrocyte differentiation by promoting apoptosis. PMID: 22198439
15. in vivo, Sema4D is an indirect regulator of bone resorption, which acts via its effect on reproductive function PMID: 22046317
16. Data show that osteoclasts express semaphorin 4D (Sema4D), previously shown to be an axon guidance molecule, which potently inhibits bone formation. PMID: 22019888
17. Studies identify Plxdc1, Sema4D, and neuropilin-1 as novel LRP1-regulated cell-signaling proteins. PMID: 20919742
18. Loss of Semaphorin 4D is associated with defect in collagen-induced platelet aggregation and an impaired vascular injury response. PMID: 20855865
19. Findings suggest that Sema4D is involved in the progression phase of atherosclerosis by accelerating intimal neovascularization, resulting in enhanced macrophage infiltration in atherosclerotic plaques. PMID: 20514420
20. Sema4D enhances inducible nitric oxide synthase (iNOS) and nitric oxide (NO) production in microglia. PMID: 20038643
21. Sema4D-plexin-B2 interactions regulate mechanisms underlying cell specification, differentiation, and migration during corticogenesis. PMID: 19948886
22. Sema4D is involved in oligodendrogenesis during development and during recovery from ischemic injury. PMID: 19472224
23. CD100, which enhances the maturation of DCs, is essential in the activation and differentiation of Ag-specific T cells. PMID: 12133937
24. Semaphorin 4D triggers invasive growth, a complex programme that includes cell dissociation, anchorage-independent growth and branching morphogenesis. PMID: 12198496
25. enhances the in vitro activation and differentiation of T cells and the in vivo generation of antigen-specific T cells PMID: 12374982
26. Sema4D is a novel inhibitory factor for axonal regeneration expressed in myelin PMID: 14534257
27. Sema4D may function as a neurite-outgrowth stimulating factor in embryonic sensory neurons. PMID: 15330859
28. Strength of B-cell antigen receptor signals is strictly tuned by the interaction of CD100 with CD72; this interaction is essential for maintaining immunological homeostasis as well as generating a proper immune response. PMID: 16113236
29. CD100 enhances pathogenetic humoral immune responses and promotes the activation of Antigen-Presenting Cells by up-regulating CD86 expression PMID: 16920982
30. proteolytic cleavage of Semaphorin 4D may provide a novel molecular mechanism by which membrane type 1-matrix metalloproteinase controls tumor-induced angiogenesis PMID: 17204469
31. semaphorin 4D has a role in platelet responses to vascular injury PMID: 17244710
32. In the ovarian follicle, the effect of Plexin-B1 is mediated by sema-4D. PMID: 17376242
33. Sema4D modulates T cell-APC interactions in allogeneic responses both in vivo and in vitro. PMID: 17950916
34. that tumor-associated macrophages (TAMs) are the main cells producing Sema4D within the tumor stroma and that their ability to produce Sema4D is critical for tumor angiogenesis and vessel maturation. PMID: 18559453
35. A functional role for CD100 antigen/Plxnb1 interactions in epithelial branching morphogenesis during organogenesis is reported. PMID: 18799546
36. loss of sema4D (semaphorin 4D) expression reduces the platelet hyperactivity otherwise found in dyslipidemia, and confers protection against the development of atherosclerosis PMID: 19390055
37. These results suggest that SHP2-mediated tyrosine dephosphorylation is an important step in Sema4D-induced axon repulsion. PMID: 19433062
38. these findings suggest a novel role of Sema4D/CD100 in the regulation of microglia activation providing a valuable neuroprotective tool PMID: 19467284
39. Mouse behavioral analyses revealed enhanced motor activity in Sema4D-deficient mice, suggesting the crucial involvement of Sema4D in the neurodevelopmental processes of the central structures mediating motor behavior in mice. PMID: 19534337
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亞細胞定位：

Cell membrane; Single-pass type I membrane protein.
蛋白家族：

Semaphorin family
組織特異性：

Strongly expressed in lymphoid tissues, especially in the thymus, as well as in the nervous tissues. Expressed in neurons and glia in the developing hippocampus.
數據庫鏈接：

KEGG: mmu:20354

STRING: 10090.ENSMUSP00000021900

UniGene: Mm.33903