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  4. Phospho-Histone H1.4 (T17) Recombinant Monoclonal Antibody

Phospho-Histone H1.4 (T17) Recombinant Monoclonal Antibody

  • 中文名稱：
    磷酸化-組蛋白 H1.4 (T17)重組抗體
  • 貨號：
    CSB-RA010380A17phHU
  • 規格：
    ￥1320
  • 圖片：
    • IHC image of CSB-RA010380A17phHU diluted at 1:100 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-RA010380A17phHU diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of MCF-7 cells with CSB-RA010380A17phHU at 1:56,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
  • 其他：

產品詳情

  • 產品描述：
    CSB-RA010380A17phHU是一款特異性識別組蛋白H1.4第17位蘇氨酸磷酸化修飾位點（T17）的高靈敏度抗體。組蛋白H1家族是染色質高級結構的關鍵調控因子，其磷酸化修飾參與細胞周期進程、DNA損傷修復及染色質動態重塑等生物學過程。該抗體經ELISA、免疫組織化學（IHC）和免疫熒光（IF）嚴格驗證，在IHC應用中推薦稀釋比為1:50-1:500，可清晰顯示組織切片中磷酸化H1.4的核內定位；IF實驗推薦使用1:30-1:200稀釋度，能夠特異性標記細胞核內靶標信號，適用于活細胞或固定樣本的亞細胞定位分析。該產品采用重組單克隆技術生產，具有高批次一致性和低交叉反應性，可廣泛應用于研究有絲分裂調控、染色質凝聚機制以及表觀遺傳修飾相關領域，為探索細胞周期異常疾病（如腫瘤發生）或DNA損傷應答通路提供可靠工具。
  • Uniprot No.：
  • 基因名：
    HIST1H1E
  • 別名：
    H1 histone family member 4 antibody; H1.4 antibody; H14_HUMAN antibody; H1E antibody; H1F4 antibody; Hist1h1e antibody; Histone 1 H1e antibody; Histone cluster 1 H1e antibody; Histone H1 antibody; Histone H1.4 antibody; Histone H1B antibody; MGC116819 antibody
  • 反應種屬：
    Human
  • 免疫原：
    A synthesized peptide
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    3E1
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, IHC, IF
  • 推薦稀釋比：
    Application Recommended Dilution
    IHC 1:50-1:500
    IF 1:30-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation.
  • 基因功能參考文獻：
    1. Results show that histones H1.2 and H1.4 were observed in MDA-MB-231 metastatic breast cancer cells. The phosphorylation at S173 of histone H1.2 and S172, S187, T18, T146, and T154 of H1.4 significantly increases during M phase suggesting that these events are cell cycle-dependent. PMID: 26209608
    2. This study identified and confirmed HIST1H1E protein changes within the postsynaptic density in schizophrenia. PMID: 25048004
    3. the N-terminal domain of H1 is an important determinant of affinity and specificity of H1-chromatin interactions. PMID: 22425985
    4. PKA-mediated H1.4S35 phosphorylation dissociates H1.4 from mitotic chromatin but also suggest that this phosphorylation is necessary for specific mitotic functions. PMID: 21852232
    5. Allele-specific underacetylation of histone H4 downstream from promoter is associated with X-inactivation in human cells. PMID: 12498347
    6. the lysine residue adjacent to the phosphorylation site found on the serine residue on the H1.4 peptide KARKSAGAAKR was also shown to be methylated, raising the question of whether the hypothesized "methyl/phos" switch could be extended to linker histones PMID: 15595731
    7. Dynamic Histone H1 Isotype 4 Methylation and Demethylation by Histone Lysine Methyltransferase G9a/KMT1C and the Jumonji Domain-containing JMJD2/KDM4 Proteins PMID: 19144645

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  • 相關疾病：
    Rahman syndrome (RMNS)
  • 亞細胞定位：
    Nucleus. Chromosome. Note=Mainly localizes in heterochromatin. Dysplays a punctuate staining pattern in the nucleus.
  • 蛋白家族：
    Histone H1/H5 family
  • 數據庫鏈接：

    HGNC: 4718

    OMIM: 142220

    KEGG: hsa:3008

    STRING: 9606.ENSP00000307705

    UniGene: Hs.248133



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