Recombinant Rat Potassium-transporting ATPase subunit beta (Atp4b)

in vitro E.coli expression system

中文名稱：

Recombinant Rat Potassium-transporting ATPase subunit beta(Atp4b)
貨號：

CSB-CF002343RA
規(guī)格：
來源：

in vitro E.coli expression system
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關產(chǎn)品
靶點詳情

產(chǎn)品詳情

基因名：

Atp4b
Uniprot No.：

P18598
別名：

Atp4b; Potassium-transporting ATPase subunit beta; Gastric H(+/K(+ ATPase subunit beta; Proton pump beta chain
種屬：

Rattus norvegicus (Rat)
蛋白長度：

full length protein
表達區(qū)域：

1-294
氨基酸序列

MAALQEKKSCSQRMAEFRQYCWNPDTGQMLGRTPARWVWISLYYAAFYVVMTGLFALCIYVLMQTIDPYTPDYQDQLKSPGVTLRPDVYGERGLQISYNISENSSWAGLTHTLHSFLAGYTPASQQDSINCSSEKYFFQETFSAPNHTKFSCKFTADMLQNCSGLVDPSFGFEEGKPCFIIKMNRIVKFLPSNNTAPRVDCTFQDDPQKPRKDIEPLQVQYYPPNGTFSLHYFPYYGKKAQPHYSNPLVAAKFLNVPKNTQVLIVCKIMADHVTFDNPHDPYEGKVEFKLTIQK
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 10xHis-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

The beta subunit of the gastric H(+)/K(+) ATPase pump which transports H(+) ions in exchange for K(+) ions across the apical membrane of parietal cells. Plays a structural and regulatory role in the assembly and membrane targeting of a functionally active pump. Within a transport cycle, the transfer of a H(+) ion across the membrane is coupled to ATP hydrolysis and is associated with a transient phosphorylation of the alpha subunit that shifts the pump conformation from inward-facing (E1) to outward-facing state (E2). Interacts with the phosphorylation domain of the alpha subunit and functions as a ratchet, stabilizing the lumenal-open E2 conformation and preventing the reverse reaction of the transport cycle.
基因功能參考文獻：
1. This study on N-terminally truncated beta-variants of the gastric H,K-ATPase provides direct evidence that the beta-N terminus assists in E2P state stabilization and that this is critical for the transport efficiency of the enzyme under in vivo conditions. PMID: 19491099
2. Non-gastric H,K-ATPase is oligomycin-sensitive and can function as H+,NH4(+)-ATPase. PMID: 16046397
3. The substitution of 5 amino acids in the low affinity ouabain binding site of non-gastric proton-potassium exchanging ATPase in order to increase ouabain binding affinity is reported. PMID: 16531406
亞細胞定位：

Apical cell membrane; Single-pass type II membrane protein. Cell membrane; Single-pass type II membrane protein.
蛋白家族：

X(+)/potassium ATPases subunit beta family
組織特異性：

Stomach.
數(shù)據(jù)庫鏈接：

KEGG: rno:24217

STRING: 10116.ENSRNOP00000025957

UniGene: Rn.10033