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  4. Recombinant Rat Potassium-transporting ATPase subunit beta (Atp4b), partial

Recombinant Rat Potassium-transporting ATPase subunit beta (Atp4b), partial

  • 中文名稱：
    Recombinant Rat Potassium-transporting ATPase subunit beta(Atp4b),partial
  • 貨號：
    CSB-YP002343RA1
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Rat Potassium-transporting ATPase subunit beta(Atp4b),partial
  • 貨號：
    CSB-EP002343RA1
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Rat Potassium-transporting ATPase subunit beta(Atp4b),partial
  • 貨號：
    CSB-EP002343RA1-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Rat Potassium-transporting ATPase subunit beta(Atp4b),partial
  • 貨號：
    CSB-BP002343RA1
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Rat Potassium-transporting ATPase subunit beta(Atp4b),partial
  • 貨號：
    CSB-MP002343RA1
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    Atp4b; Potassium-transporting ATPase subunit beta; Gastric H(+/K(+ ATPase subunit beta; Proton pump beta chain
  • 種屬：
    Rattus norvegicus (Rat)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    The beta subunit of the gastric H(+)/K(+) ATPase pump which transports H(+) ions in exchange for K(+) ions across the apical membrane of parietal cells. Plays a structural and regulatory role in the assembly and membrane targeting of a functionally active pump. Within a transport cycle, the transfer of a H(+) ion across the membrane is coupled to ATP hydrolysis and is associated with a transient phosphorylation of the alpha subunit that shifts the pump conformation from inward-facing (E1) to outward-facing state (E2). Interacts with the phosphorylation domain of the alpha subunit and functions as a ratchet, stabilizing the lumenal-open E2 conformation and preventing the reverse reaction of the transport cycle.
  • 基因功能參考文獻：
    1. This study on N-terminally truncated beta-variants of the gastric H,K-ATPase provides direct evidence that the beta-N terminus assists in E2P state stabilization and that this is critical for the transport efficiency of the enzyme under in vivo conditions. PMID: 19491099
    2. Non-gastric H,K-ATPase is oligomycin-sensitive and can function as H+,NH4(+)-ATPase. PMID: 16046397
    3. The substitution of 5 amino acids in the low affinity ouabain binding site of non-gastric proton-potassium exchanging ATPase in order to increase ouabain binding affinity is reported. PMID: 16531406
  • 亞細胞定位：
    Apical cell membrane; Single-pass type II membrane protein. Cell membrane; Single-pass type II membrane protein.
  • 蛋白家族：
    X(+)/potassium ATPases subunit beta family
  • 組織特異性：
    Stomach.
  • 數據庫鏈接：


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