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Recombinant Bovine Lens fiber major intrinsic protein (MIP)

  • 中文名稱:
    牛MIP重組蛋白
  • 貨號:
    CSB-CF013834BO
  • 規格:
  • 來源:
    in vitro E.coli expression system
  • 其他:

產品詳情

  • 基因名:
    MIP
  • Uniprot No.:
  • 別名:
    MIP; Lens fiber major intrinsic protein; Aquaporin-0; MIP26; MP26
  • 種屬:
    Bos taurus (Bovine)
  • 蛋白長度:
    Full length protein
  • 表達區域:
    1-263
  • 氨基酸序列
    MWELRSASFWRAICAEFFASLFYVFFGLGASLRWAPGPLHVLQVALAFGLALATLVQAVGHISGAHVNPAVTFAFLVGSQMSLLRAICYMVAQLLGAVAGAAVLYSVTPPAVRGNLALNTLHPGVSVGQATIVEIFLTLQFVLCIFATYDERRNGRLGSVALAVGFSLTLGHLFGMYYTGAGMNPARSFAPAILTRNFTNHWVYWVGPVIGAGLGSLLYDFLLFPRLKSVSERLSILKGSRPSESNGQPEVTGEPVELKTQAL
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標簽:
    N-terminal 10xHis-tagged
  • 產品提供形式:
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液:
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA:
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Water channel. Channel activity is down-regulated by CALM when cytoplasmic Ca(2+) levels are increased. May be responsible for regulating the osmolarity of the lens. Interactions between homotetramers from adjoining membranes may stabilize cell junctions in the eye lens core. Plays a role in cell-to-cell adhesion and facilitates gap junction coupling.
  • 基因功能參考文獻:
    1. The data evidence a broad lipidation profile of AQP0 that is both species and site independent, suggesting a chemical-based ester aminolysis mechanism to explain such modifications. PMID: 27378310
    2. In the presence of alpha-crystallin, this conversion to beta-sheet is minimized, suggesting that the protein structure that binds to the molecular chaperone is mostly the alpha-helical structure of AQP0. PMID: 25044119
    3. Determined the pseudoatomic structure of full-length aquaporin-0 (AQP0) in complex with calmodulin, using electron microscopy to elucidate how this signaling protein modulates water-channel function. PMID: 23893133
    4. Tyr23 is a dominate factor leading to the low water permeability in AQP0. PMID: 19961829
    5. AQP-0 and connexins can be segregated in the membrane by protein-lipid interactions as modified by AQP-0 homo-oligomerization PMID: 19883592
    6. determination of the x-ray structure of bovine aquaporin 0 to a resolution of 2.2 A; structure aids the analysis of the interaction of the extracellular domains and the possibility of a cell-cell adhesion role for AQP0 PMID: 15377788
    7. The crystal packing was determined by molecular replacement and shows that, within the cubic lattice, AQP0 tetramers are associated head-to-head along their 4-fold axes. PMID: 16309700

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  • 亞細胞定位:
    Cell membrane; Multi-pass membrane protein. Cell junction, gap junction.
  • 蛋白家族:
    MIP/aquaporin (TC 1.A.8) family
  • 組織特異性:
    Major component of lens fiber gap junctions.
  • 數據庫鏈接:


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