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  4. Recombinant Zaire ebolavirus Pre-small/secreted glycoprotein (GP)

Recombinant Zaire ebolavirus Pre-small/secreted glycoprotein (GP)

  • 中文名稱：
    Recombinant Zaire ebolavirus Pre-small/secreted glycoprotein(GP)
  • 貨號：
    CSB-BP350772ZAT
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Zaire ebolavirus Pre-small/secreted glycoprotein(GP)
  • 貨號：
    CSB-EP350772ZAT-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Zaire ebolavirus Pre-small/secreted glycoprotein(GP)
  • 貨號：
    CSB-MP350772ZAT
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    Name:GP
  • Uniprot No.：
  • 別名：
    GP; Pre-small/secreted glycoprotein; pre-sGP) [Cleaved into: Small/secreted glycoprotein; sGP); Delta-peptide]
  • 種屬：
    Zaire ebolavirus (strain Kikwit-95) (ZEBOV) (Zaire Ebola virus)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區域：
    33-364
  • 氨基酸序列
    IPLGVIHN STLQVSDVDK LVCRDKLSST NQLRSVGLNL EGNGVATDVP SATKRWGFRS GVPPKVVNYE AGEWAENCYN LEIKKPDGSE CLPAAPDGIR GFPRCRYVHK VSGTGPCAGD FAFHKEGAFF LYDRLASTVI YRGTTFAEGV VAFLILPQAK KDFFSSHPLR EPVNATEDPS SGYYSTTIRY QATGFGTNET EYLFEVDNLT YVQLESRFTP QFLLQLNETI YTSGKRSNTT GKLIWKVNPE IDTTIGEWAF WETKKTSLEK FAVKSCLSQL YQTEPKTSVV RVRRELLPTQ GPTQQLKTTK SWLQKIPLQW FKCTVKEGKL QCRI
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    sGP seems to possess an anti-inflammatory activity as it can reverse the barrier-decreasing effects of TNF alpha. Might therefore contribute to the lack of inflammatory reaction seen during infection in spite the of extensive necrosis and massive virus production. Does not seem to be involved in activation of primary macrophages. Does not seem to interact specifically with neutrophils.; Viroporin that permeabilizes mammalian cell plasma membranes. It acts by altering permeation of ionic compounds and small molecules. This activity may lead to viral enterotoxic activity.
  • 基因功能參考文獻：
    1. Growth-Adaptive Mutations in the Ebola Virus Makona Glycoprotein Alter Different Steps in the Virus Entry Pathway. PMID: 30021890
    2. the structure of the membrane proximal external region (MPER) connected to the TM domain: i.e., the missing parts of the EBOV GP2 structure. The structure, solved by solution NMR and EPR spectroscopy in membrane-mimetic environments, consists of a helix-turn-helix architecture that is independent of pH. PMID: 28874543
    3. Ebola virus GP directly subverts the host's immune response by increasing the susceptibility of monocytes to Ebola virus infection. PMID: 28542576
    4. report provides experimental evidence that the spontaneous mutation T544I is a tissue culture adaptation in certain cell lines and that it may be unique for the species Zaire ebolavirus; the mutation led to a marked increase in virus growth kinetics and conferred a robust growth advantage over wild-type rVSV-MAK-GP on Vero E6 cells PMID: 28539437
    5. although Asn(563) and Asn(618) are not required for EBOV GP expression, they synergistically regulate its maturation, which determines its functionality. PMID: 28196864
    6. Study determined the crystal structure of the primed GP (GPcl) of Ebola virus bound to domain C of NPC1 (NPC1-C); NPC1-C utilizes two protruding loops to engage a hydrophobic cavity on head of GPcl. Upon enzymatic cleavage and NPC1-C binding, conformational change in the GPcl further affects the state of the internal fusion loop, triggering membrane fusion. PMID: 26771495
    7. GP1,2 expression levels have a profound effect on factors that contribute to virus fitness and RNA editing may be an important mechanism employed by EBOV to regulate GP1,2 expression in order to optimize virus production and infectivity. PMID: 25392212
    8. Mucin-like domain is located at the apex and the sides of each glycoprotein monomer. PMID: 25008940
    9. The glycoprotein's internal fusion loop is critical for viral entry and fusion. PMID: 24696482
    10. GP play multiple functions including virus attachment and entry, cell rounding and cytotoxicity and down-regulation of host surface proteins.[review] PMID: 23757858
    11. findings demonstrate that a GP mutant, GP-F88A, defective for entry into a variety of human cell types, including antigen-presenting cells (APCs) can mediate viral entry into mouse CD11b( ) APCs; studies suggest an important role for NPC1 in the differential entry of GP-F88A into mouse versus human APCs PMID: 23302883
    12. Purified human NPC1 binds only to a cleaved form of Ebola virus spike glycoprotein that is generated within cells during entry, and only viruses containing cleaved glycoprotein can utilize a receptor retargeted to the cell surface. PMID: 22395071
    13. Ebola virus GP forms a tandem beta-hairpin structure that binds deeply into a cleft in the antibody-combining site. PMID: 22171276
    14. Cathepsins B and L activate Ebola but not Marburg virus glycoproteins for efficient entry into cell lines and macrophages independent of TMPRSS2 expression. PMID: 22222211
    15. Cathepsin L cleavage potentiates the Ebola virus GP to undergo a fusion-relevant conformational change. PMID: 22031933
    16. R64 and K95 of GP1 are involved in receptor binding. PMID: 21667336
    17. liposome fusion data and NMR structures for a complete (54-residue) disulfide-bonded internal fusion loop (Ebov FL) in a membrane mimetic PMID: 21690393
    18. Axl enhances entry of Zaire ebolavirus without direct interactions with the viral glycoprotein. PMID: 21529875
    19. While ssGP appears to share similar structural properties with sGP, it does not appear to have the same anti-inflammatory function on endothelial cells as sGP. PMID: 21411529
    20. These findings suggest that Ebola virus GP uses a novel mechanism to circumvent tetherin restriction. PMID: 20444895
    21. CatB cleavage is required to facilitate the triggering of viral membrane fusion by destabilizing the prefusion conformation of EBOV GP PMID: 19846533
    22. Ebola virus GP cytotoxicity is regulated by dynamin-dependent cellular protein trafficking PMID: 15596847
    23. The transmembrane region of GP2 modifies the permeability of the plasma membrane. PMID: 16927113
    24. the crystal structure of EBOV GP in its trimeric, pre-fusion conformation (GP1+GP2) bound to a neutralizing antibody, KZ52, derived from a human survivor of the 1995 Kikwit outbreak PMID: 18615077
    25. The authors show that the mucin domain from the highly pathogenic Zaire subtype of Ebola virus is sufficient to cause characteristic cytopathology when expressed in the context of a foreign glycoprotein. PMID: 19013626

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  • 亞細胞定位：
    [Small/secreted glycoprotein]: Secreted.; [Delta-peptide]: Secreted.
  • 蛋白家族：
    Filoviruses glycoprotein family


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