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  4. Recombinant Xenopus tropicalis Folliculin (flcn), partial

Recombinant Xenopus tropicalis Folliculin (flcn), partial

  • 中文名稱：
    熱帶爪蟾flcn重組蛋白
  • 貨號：
    CSB-YP008711XBF
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    熱帶爪蟾flcn重組蛋白
  • 貨號：
    CSB-EP008711XBF
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    熱帶爪蟾flcn重組蛋白
  • 貨號：
    CSB-EP008711XBF-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    熱帶爪蟾flcn重組蛋白
  • 貨號：
    CSB-BP008711XBF
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    熱帶爪蟾flcn重組蛋白
  • 貨號：
    CSB-MP008711XBF
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    flcn; Folliculin
  • 種屬：
    Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    GTPase-activating protein that plays a key role in the cellular response to amino acid availability through regulation of the mTORC1 signaling cascade controlling the MiT/TFE factors tfeb and tfe3. Activates mTORC1 by acting as a GTPase-activating protein: specifically stimulates GTP hydrolysis by rragc/RagC or rragd/RagD, promoting the conversion to the GDP-bound state of rragc/RagC or rragd/RagD, and thereby activating the kinase activity of mTORC1. The GTPase-activating activity is inhibited during starvation and activated in presence of nutrients. In low-amino acid conditions, the lysosomal folliculin complex (LFC) is formed on the membrane of lysosomes, which inhibits the GTPase-activating activity of flcn, inactivates mTORC1 and maximizes nuclear translocation of tfeb and tfe3. Upon amino acid restimulation, rraga/RagA (or rragb/RagB) nucleotide exchange promotes disassembly of the LFC complex and liberates the GTPase-activating activity of flcn, leading to activation of mTORC1 and subsequent cytoplasmic retention of tfeb and tfe3. Required for the exit of hematopoietic stem cell from pluripotency by promoting mTOR-dependent cytoplasmic retention of tfe3, thereby increasing Wnt signaling. Acts as an inhibitor of browning of adipose tissue by regulating mTOR-dependent cytoplasmic retention of tfe3. In response to flow stress, regulates STK11/LKB1 accumulation and mTORC1 activation through primary cilia. Required for starvation-induced perinuclear clustering of lysosomes by promoting association of rilp with its effector rab34.
  • 亞細胞定位：
    Lysosome membrane. Cytoplasm, cytosol. Cell projection, cilium. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Nucleus.
  • 蛋白家族：
    Folliculin family
  • 數據庫鏈接：


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