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  4. Recombinant Xenopus laevis Probable E3 ubiquitin-protein ligase makorin-2 (mkrn2)

Recombinant Xenopus laevis Probable E3 ubiquitin-protein ligase makorin-2 (mkrn2)

  • 中文名稱：
    有爪蟾蜍mkrn2重組蛋白
  • 貨號：
    CSB-YP014606XBE
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    有爪蟾蜍mkrn2重組蛋白
  • 貨號：
    CSB-EP014606XBE
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    有爪蟾蜍mkrn2重組蛋白
  • 貨號：
    CSB-EP014606XBE-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    有爪蟾蜍mkrn2重組蛋白
  • 貨號：
    CSB-BP014606XBE
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    有爪蟾蜍mkrn2重組蛋白
  • 貨號：
    CSB-MP014606XBE
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    mkrn2
  • Uniprot No.：
  • 別名：
    mkrn2Probable E3 ubiquitin-protein ligase makorin-2; EC 2.3.2.27; RING-type E3 ubiquitin transferase makorin-2
  • 種屬：
    Xenopus laevis (African clawed frog)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-409
  • 氨基酸序列
    MSPKQVTCRY FLHGVCREGS RCLFSHDLAT SKPSTVCRFF LRGQCAYGTR CRYDHVKPCN GTVFIPPQEM SPVLSPPPLF PAQEAAVPPT IPAPQRREKK TLVLRDRDLC GASVDPALQP GCITESQGSE GEAKPHSYLE AICTGLDESQ DPASYPGAPQ QLCPFAQAGE CHCGDSCPYL HGDACEICGL QVLHPHDQEQ RRDHEKLCME NFELDMERAF AVQASEGRVC SICMERVYEK QSPAQRRFRI LSDCNHTYCL TCIRQWRCAR QFDNPVIKSC PECRVISEFV IPSAYWVEDQ SKKDELIEAF KQGMGKKLCK YFDQGRGTCP FGGKCLYLHS YPDGTRAQPE KPRKQLGSEG SVRFLNSLRL WDFIEDREQR GVPNAEVGKL GELFMHLSGA DEELPAPFN
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Inhibits neurogenesis and axis formation during embryonic development by modulating the phosphatidylinositol 3-kinase (PI3K) pathway. Acts downstream of PI3K and akt1 to up-regulate gsk3b mRNA expression.
  • 基因功能參考文獻：
    1. These results collectively suggest that the third C(3)H zinc finger, Cys-His motif and C(3)HC(4) RING zinc finger are indispensable for the anti-neurogenic activity of mkrn2. PMID: 20167204
    2. mkrn2 acts downstream of PI3K and Akt and upstream of GSK-3beta PMID: 18198183
  • 組織特異性：
    In adult males, highly expressed in testis, small intestine, large intestine, stomach, heart, liver, kidney, lung and muscle. Consistent with its role as a neurogenic inhibitor, expression is much lower in the brain, and also in skin.
  • 數據庫鏈接：

    KEGG: xla:100141485

    UniGene: Xl.76333



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