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Recombinant Streptococcus pneumoniae serotype 2 Pneumolysin (ply)

In Stock
  • 中文名稱:
    Recombinant Streptococcus pneumoniae serotype 2 Pneumolysin(ply)
  • 貨號:
    CSB-EP312102FNF
  • 規格:
    ¥2328
  • 圖片:
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP312102FNF could indicate that this peptide derived from E.coli-expressed Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466) ply.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP312102FNF could indicate that this peptide derived from E.coli-expressed Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466) ply.
  • 其他:

產品詳情

  • 純度:
    Greater than 90% as determined by SDS-PAGE.
  • 基因名:
    ply
  • Uniprot No.:
  • 別名:
    ply; SPD_1726; Pneumolysin; Thiol-activated cytolysin
  • 種屬:
    Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466)
  • 蛋白長度:
    Full Length of Mature Protein
  • 來源:
    E.coli
  • 分子量:
    72.8kDa
  • 表達區域:
    2-471aa
  • 氨基酸序列
    ANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFVVIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSIDLPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQLKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTVTVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQTEWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTSFLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWDELSYDHQGKEVLTPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRKRTISIWGTTLYPQVEDKVEND
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標簽:
    N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged
  • 產品提供形式:
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液:
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
    Note: If you have any special requirement for the glycerol content, please remark when you place the order.
    If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    3-7 business days
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 產品描述:

    The LC-MS/MS Analysis-validated E.coli-expressed Recombinant Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466) (ply) protein is the full-length of mature protein with an N-terminal 10xHis-SUMO-tag and a C-terminal Myc-tag. The expression region corresponds to 2-471aa of Streptococcus pneumoniae serotype 2 ply protein. This recombinant ply protein has a molecular weight ranging from 66.2 kDa to 92 kDa and purity greater than 90% as determined by SDS-PAGE.

    Pneumolysin (ply) is a toxic protein antigenically related to thiol-activated, cholesterol-binding cytolysins. High levels of ply can lyse all cells with cholesterol-containing membranes. Ply participates in the pathogenesis of lethal pneumococcal pneumonia probably due to its pro-apoptotic and pro-inflammatory activity.

  • Datasheet & COA:
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    A cholesterol-dependent toxin that causes cytolysis by forming pores in cholesterol-containing host membranes. After binding to target membranes, the protein undergoes a major conformation change, leading to its insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol is required for binding to host membranes, membrane insertion and pore formation; cholesterol binding is mediated by a Thr-Leu pair in the C-terminus. Can be reversibly inactivated by oxidation.
  • 亞細胞定位:
    Secreted. Host cell membrane; Multi-pass membrane protein.
  • 蛋白家族:
    Thiol-activated cytolysin family
  • 數據庫鏈接:


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