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Recombinant Human Myosin regulatory light chain 12B (MYL12B) (Active)

In Stock
  • 中文名稱:
    人MYL12B重組蛋白
  • 貨號:
    CSB-EP015308HUc7
  • 規(guī)格:
    ¥1836
  • 圖片:
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Activity
      Measured by its binding ability in a functional ELISA. Immobilized Human MYL12B at 2μg/mL can bind Anti-MYL9 recombinant antibody (CSB-RA015318MA1HU),the EC50 is 19.290-28.646 ng/mL. Biological Activity Assay
  • 其他:

產(chǎn)品詳情

  • 純度:
    Greater than 95% as determined by SDS-PAGE.
  • 內(nèi)毒素:
    Less than 1.0 EU/ug as determined by LAL method.
  • 生物活性:
    Measured by its binding ability in a functional ELISA. Immobilized Human MYL12B at 2 μg/mL can bind Anti-MYL9 recombinant antibody (CSB-RA015318MA1HU). The EC50 is 19.290-28.646 ng/mL.
  • 基因名:
  • Uniprot No.:
  • 別名:
    MRLC2;MYLC2B
  • 種屬:
    Homo sapiens (Human)
  • 蛋白長度:
    Full Length
  • 來源:
    E.coli
  • 分子量:
    26.7 kDa
  • 表達(dá)區(qū)域:
    1-172aa
  • 氨基酸序列
    MSSKKAKTKTTKKRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDAYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGTIQEDYLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDKDD
  • 蛋白標(biāo)簽:
    C-terminal 6xHis-tagged
  • 產(chǎn)品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液:
    Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
  • 復(fù)溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    3-7 business days
  • 注意事項(xiàng):
    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
  • Datasheet & COA:
    Please contact us to get it.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能:
    Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Phosphorylation triggers actin polymerization in vascular smooth muscle. Implicated in cytokinesis, receptor capping, and cell locomotion.
  • 基因功能參考文獻(xiàn):
    1. phosphorylated MRLC temporally controls its own accumulation, but not that of actin, in cultured mammalian cells. PMID: 22374324
    2. The study data show that manipulation of the activation sites (Thr18/Ser19) significantly alters myosin II function in a number of these assays while manipulation of the putative inhibitory sites (Ser1/Ser2/Thr9) does not. PMID: 22136066
    3. These results suggest that 2P-MRLC has a role different from that of 1P-MRLC at the midzone, and is not a subunit of myosin II. PMID: 22166199
    4. These results suggest that EGCG inhibits the cell growth by reducing the MRLC phosphorylation and this effect is mediated by the 67LR. PMID: 15946647
  • 組織特異性:
    Ubiquitously expressed in various hematopoietic cells.
  • 數(shù)據(jù)庫鏈接:

    HGNC: 29827

    KEGG: hsa:103910

    STRING: 9606.ENSP00000237500

    UniGene: Hs.190086



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