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  4. Recombinant Schizosaccharomyces pombe Uncharacterized ATP-dependent helicase C25A8.01c (SPAC25A8.01c), partial

Recombinant Schizosaccharomyces pombe Uncharacterized ATP-dependent helicase C25A8.01c (SPAC25A8.01c), partial

  • 中文名稱：
    龐貝裂殖酵母fft3重組蛋白
  • 貨號：
    CSB-YP528563SXV
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母fft3重組蛋白
  • 貨號：
    CSB-EP528563SXV
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母fft3重組蛋白
  • 貨號：
    CSB-EP528563SXV-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    龐貝裂殖酵母fft3重組蛋白
  • 貨號：
    CSB-BP528563SXV
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母fft3重組蛋白
  • 貨號：
    CSB-MP528563SXV
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    fft3
  • Uniprot No.：
  • 別名：
    fft3; snf2SR; SPAC25A8.01cATP-dependent helicase fft3; EC 3.6.4.12; Fun thirty-related protein 3
  • 種屬：
    Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    DNA helicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity and is required for heterochromatin organization. Required for maintaining a heterochromatin chromatin structure at centromeres and subtelomeres by protecting these regions from euchromatin assembly. Enhances the nucleotide exchange activity of the pim1 guanine nucleotide exchange factor and abolishes histone-H3-mediated RanGAP inhibition. Involved in the construction of the centromeres.
  • 基因功能參考文獻：
    1. Data indicate Fun30(Fft3) as a chromatin remodeller, which localizes at transcribing regions to promote RNA polymerase II (RNAPII) transcription. PMID: 28218250
    2. These analyses of the regulation of nucleosome dynamics by Fun30(Fft3) during RNA polymerase II (RNAPII) -mediated transcription reveal an unprecedented role for a chromatin remodeler in nucleosome disassembly at transcribing regions. They offer a new conceptual framework that can describe, in mathematical terms, how nucleosome dynamics are controlled during RNAPII-mediated elongation. PMID: 28925811
    3. This work uncovers a conserved factor critical for epigenetic inheritance of heterochromatin and describes a mechanism in which Fft3 suppresses nucleosome turnover, preventing formation of structural barriers that impede replication at fragile regions in the genome. PMID: 28318821
    4. We propose that Fft3 has a global role in mediating association between specific chromatin domains and the nuclear envelope PMID: 25798942
    5. Fft3 controls the identity of chromatin domains by protecting these regions from euchromatin assembly. PMID: 21437270
    6. Results indicate that Snf2SR is involved in the Ran GTPase cycle in vivo. PMID: 18422602

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  • 亞細胞定位：
    Nucleus. Chromosome.
  • 蛋白家族：
    SNF2/RAD54 helicase family
  • 數據庫鏈接：


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