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  4. Recombinant Schizosaccharomyces pombe Replication termination factor 1 (rtf1)

Recombinant Schizosaccharomyces pombe Replication termination factor 1 (rtf1)

  • 中文名稱：
    龐貝裂殖酵母rtf1重組蛋白
  • 貨號：
    CSB-YP892441SXV
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母rtf1重組蛋白
  • 貨號：
    CSB-EP892441SXV
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母rtf1重組蛋白
  • 貨號：
    CSB-EP892441SXV-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    龐貝裂殖酵母rtf1重組蛋白
  • 貨號：
    CSB-BP892441SXV
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母rtf1重組蛋白
  • 貨號：
    CSB-MP892441SXV
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    rtf1
  • Uniprot No.：
  • 別名：
    rtf1; SPAC22F8.07c; Replication termination factor 1
  • 種屬：
    Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-466
  • 氨基酸序列
    MQGKNNLSCR PDTEDNEELF VDDQLLSPIG DSKNTSSFIY LGNPISFHEY NYDETMVSPE NVKTAIAGSA KDHETCRGFK KTGTTSYKDF VFSRDYTNWT PTFWVLLSQL IDEFLKESEL NFVAARDLLI KTKRLPKPFN NLLIQFQIQV PNVSRRTVYR HLKGYFNIPG YERFQYVKKA SSGSWGANDI ITLEKEIAMF KKKKNWSDEQ FLQYVWSDNH RDEMKTLYNC LYELIDRDKK SIYNYLRRKY NPFKKKCKWT IEDEAELKKL VEKHGTSWSL IGKLSNRLPM HCRDHWRDYI QPGEINRSPW TIQEKEKLIK TVNQYLQSNP SSPIQWSLIS KNMRNRHRHH CRWKYYTLIS RDIHNSSPFK LGDSIWLIER MMDLNVAEER MIDWKCLSEY ANHLWTADAC KSHFERIKKT LFIDGLSTFS DTLIHLHKML NSSPEETYIS NLHDSYTAFS NADDLC
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Mediates site-specific replication termination at the polar replication barrier RTS1, a barrier which ensures that replication of the mat1 locus in S.pombe occurs in the centromere-proximal direction.
  • 基因功能參考文獻：
    1. We propose that this opposition constitutes a Cdk9 auto-regulatory mechanism, such that a positive effect on elongation, driven by the PAF pathway, is kept in check by a negative effect of Prf1/Rtf1 and downstream mono-ubiquitylation of histone H2B PMID: 24385927
    2. multiple protein DNA and protein-protein interactions between Rtf1 molecules and both the repeated motifs and the enhancer region of RTS1 are required for site-specific termination at the RTS1 element PMID: 18723894
    3. Rtf2 stabilizes the replication fork stalled at RTS1 until completion of DNA synthesis by a converging replication fork initiated at a flanking origin PMID: 19416828
  • 亞細胞定位：
    Nucleus.
  • 數據庫鏈接：


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