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  4. Recombinant Schizosaccharomyces pombe Cell cycle serine/threonine-protein kinase hsk1 (hsk1), partial

Recombinant Schizosaccharomyces pombe Cell cycle serine/threonine-protein kinase hsk1 (hsk1), partial

  • 中文名稱：
    龐貝裂殖酵母hsk1重組蛋白
  • 貨號：
    CSB-YP343866SXV
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母hsk1重組蛋白
  • 貨號：
    CSB-EP343866SXV
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母hsk1重組蛋白
  • 貨號：
    CSB-EP343866SXV-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    龐貝裂殖酵母hsk1重組蛋白
  • 貨號：
    CSB-BP343866SXV
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母hsk1重組蛋白
  • 貨號：
    CSB-MP343866SXV
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    hsk1
  • Uniprot No.：
  • 別名：
    hsk1; SPBC776.12cCell cycle serine/threonine-protein kinase hsk1; EC 2.7.11.1; Cdc7-related kinase; Minichromosome maintenance protein kinase
  • 種屬：
    Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Required for G1/S transition. Plays a role in DNA replication checkpoint signaling through regulating rad3 and cds1. Involved in the maintenance of mitotic chromosome structures during S phase through regulating the function of rad21. Required for initiation of mitotic DNA replication through phosphorylating mcm2/cdc19. Required for genome integrity.
  • 基因功能參考文獻：
    1. Multiple pathways can bypass the essential role of fission yeast Hsk1 kinase in DNA replication initiation PMID: 22024164
    2. Hsk1 kinase activity is required for induction and maintenance of Mrc1 hyperphosphorylation, which is induced by replication fork block and mediated by Rad3. PMID: 21099360
    3. Hsk1-Dfp1 kinase functions post-initiation to maintain replication fork stability, an activity potentially mediated by the C terminus of Dfp1. PMID: 20176980
    4. Swi1, Swi3, and Hsk1 act in a novel S-phase checkpoint pathway PMID: 15767681
    5. Hsk1 kinase has roles in the initiation of meiotic recombination and meiosis PMID: 16698922
    6. These results support a model in which the accessibility of replication origins to Hsk1-Dfp1 regulates origin efficiency and provides a potential mechanistic link between chromatin structure and replication timing. PMID: 18799612
    7. These results suggest that interactions of the Swi1-Swi3 complex and Hsk1 kinase with Mrc1 may play a role in cellular responses to stalled replication forks in fission yeast. PMID: 19422421

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  • 亞細胞定位：
    Nucleus. Note=Nuclear throughout the cell cycle.
  • 蛋白家族：
    Protein kinase superfamily, Ser/Thr protein kinase family, CDC7 subfamily
  • 數據庫鏈接：


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