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  4. Recombinant Saccharomyces cerevisiae Importin subunit beta-4 (KAP123), partial

Recombinant Saccharomyces cerevisiae Importin subunit beta-4 (KAP123), partial

  • 中文名稱：
    釀酒酵母KAP123重組蛋白
  • 貨號：
    CSB-YP336672SVG
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母KAP123重組蛋白
  • 貨號：
    CSB-EP336672SVG
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母KAP123重組蛋白
  • 貨號：
    CSB-EP336672SVG-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母KAP123重組蛋白
  • 貨號：
    CSB-BP336672SVG
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母KAP123重組蛋白
  • 貨號：
    CSB-MP336672SVG
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    KAP123
  • Uniprot No.：
  • 別名：
    KAP123; YRB4; YER110CImportin subunit beta-4; Importin-123; Karyopherin subunit beta-4; Karyopherin-123; Ran-binding protein YRB4
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Its predominant cargo substrate seems to be ribosomal proteins. Required for import of the ribosomal assembly factor NMD3. May be involved in nuclear transport of YAP1. Mediates the nuclear import of histones H3 and H4. Mediates docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to repeat-containing nucleoporins. The complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, GTP-Ran binding leads to release of the cargo. The importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.
  • 基因功能參考文獻：
    1. The karyopherin Kap123 binds to a nuclear localization sequence next to this amphipathic alpha-helix and prevents unspecific tethering of Nbp1 to membranes. PMID: 21785410
    2. Slt2, the mitogen-activated protein kinase of the protein kinase C cell integrity pathway, is constitutively activated in the absence of Kap123, which is consistent with the existence of cell integrity defects. PMID: 19930464
    3. A role for the karyopherin Kap123p in microtubule stability is reported. PMID: 19761543
    4. The Kap123p specifically binds to histones H2A and H2B. PMID: 15679097
  • 亞細胞定位：
    Cytoplasm. Nucleus. Nucleus, nuclear pore complex.
  • 蛋白家族：
    Importin beta family
  • 數據庫鏈接：

    KEGG: sce:YER110C

    STRING: 4932.YER110C



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