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  4. Recombinant Saccharomyces cerevisiae Flocculation protein FLO11 (MUC1), partial

Recombinant Saccharomyces cerevisiae Flocculation protein FLO11 (MUC1), partial

  • 中文名稱：
    釀酒酵母FLO11重組蛋白
  • 貨號：
    CSB-YP357442SVG
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母FLO11重組蛋白
  • 貨號：
    CSB-EP357442SVG
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母FLO11重組蛋白
  • 貨號：
    CSB-EP357442SVG-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母FLO11重組蛋白
  • 貨號：
    CSB-BP357442SVG
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母FLO11重組蛋白
  • 貨號：
    CSB-MP357442SVG
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    FLO11
  • Uniprot No.：
  • 別名：
    FLO11; MAL5; MUC1; S1; S2; YIR019CFlocculation protein FLO11; Flo11p; Flocculin-11; Mucin-like protein 1
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Homophilic binding protein that enables kin discrimination in heterogeneous yeast populations by mediating homotypic cell-cell interactions during flocculation, a reversible and asexual process in which cells adhere to form aggregates (flocs). Plays a role in cell-substrate adhesion, haploid invasive growth, diploid pseudohyphae formation and biofilm (flor) development. Adhesive activity is inhibited by mannose, but not by glucose, maltose, sucrose or galactose.
  • 基因功能參考文獻：
    1. by deleting many of the known transcriptional activators of invasion, as well as the gene encoding the cell surface protein Flo11 from five relevant segregants and showing that these individuals differ in the genes they require for invasion PMID: 26232408
    2. Interactions by the fungal Flo11 adhesin depend on a fibronectin type III-like adhesin domain girdled by aromatic bands. PMID: 25960408
    3. Flo11 downregulation causes filamentous and invasive growth of rho mutants. PMID: 23172851
    4. findings show that Flo11p is covalently associated with the cell wall and shed into the extracellular matrix of the growing mat. PMID: 21908597
    5. Surface plasmon resonance analysis showed that the N-terminal domain of Flo11p self-interacts and explains the cell-cell interaction capacity of FLO11-expressing cells. PMID: 22129043
    6. Adhesin Flo11p participates in the formation of extracellular fibers which in biofilms interconnect oval cells in the upper layer and pseudohyphae in the roots. PMID: 21875945
    7. A flo11Delta mutant, which fails to form pseudohyphae, yielded normal sporulation in cultures, but was defective for minicolony sporulation. PMID: 21205160
    8. FLO11 is an essential gene involved in pellicle formation by wine spoilage wild yeasts. PMID: 20926338
    9. Two alleles of 3.1 and 5.0 kb were cloned into strain BY4742 to compare the FLO11-associated phenotypes in the same genetic background. There is a significant correlation between biofilm-forming ability and FLO11 length. PMID: 19729408
    10. Results describe the ability of tailor-made zinc finger proteins to activate the expression of the FLO11 gene in Saccharomyces cerevisiae , with phenotypic consequences. PMID: 17710146
    11. FLO11 has a role in biofilm formation in Saccharomyces cerevisiae. PMID: 15321692
    12. required for air-liquid interfacial biofilm formation during growth of wine strain on ethanol PMID: 15932987
    13. Flo11p-dependent flocculation revealed is dependent on calcium, occurs only at cell densities greater than 1 x 10(8) ml(-1), and only occurs at acidic pH. PMID: 16043420
    14. disruptant (Deltaflo11) shows lower hydrophobicity of cell surface than the wild type PMID: 16556982
    15. analysis of adaptive evolution mutations in the FLO11 gene PMID: 16844788
    16. we present a modular steady state approach to quantify the network comprising of cAMP-PKA and MAP kinase pathways involved in the regulation of FLO11, a gene which is required for pseudohyphae growth in Saccharomyces cerevisiae under nitrogen starvation. PMID: 16863676
    17. These results indicate that Opi1p affects mat formation and invasive growth by participating in the regulation of FLO11. PMID: 16896211
    18. Deletion of CPC2/ASC1 abolished amino acid starvation-induced adhesive growth and impaired basal expression of FLO11 and its activation upon starvation in haploid cells. PMID: 17704055
    19. Flo11/Muc1 is a Saccharomyces cerevisiae mannoprotein with homotypic properties of adhesion PMID: 17921350
    20. biofilm formation and sliding motility were shown to be strictly dependent on the cell-surface adhesin Flo11p PMID: 17947402
    21. It is therefore concluded that low levels of FLO11 transcripts are essential and sufficient for derepression of FLO11 expression and adhesive growth during amino acid starvation. PMID: 17999676
    22. Several genes whose function was essential to maintain the expression of FLO11, were isolated. PMID: 18202364
    23. global signaling pathways respond with specific sensitivity to regulate the expression of FLO11 under nitrogen limitation. PMID: 18301741
    24. study reports that, under optimal laboratory conditions, the repeats domain of the FLO11 gene in wild yeasts is unstable; length variations in this gene provide a combinatorial diversity which may contribute to rapid adaptation to fluctuating environments PMID: 19160455
    25. FLO11 switch between a silenced and competent promoter state in a random and independent fashion. PMID: 19798446
    26. demonstrate that Rpd3L activates FLO11 transcription via its repressive effects on one of the cis-acting noncoding RNAs that itself negatively regulates FLO11 transcription. PMID: 19805129

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  • 亞細胞定位：
    Secreted, cell wall. Membrane; Lipid-anchor, GPI-anchor.
  • 蛋白家族：
    Flocculin family
  • 數據庫鏈接：

    KEGG: sce:YIR019C

    STRING: 4932.YIR019C



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