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  4. Recombinant Saccharomyces cerevisiae Endosomal/prevacuolar sodium/hydrogen exchanger (NHX1), partial

Recombinant Saccharomyces cerevisiae Endosomal/prevacuolar sodium/hydrogen exchanger (NHX1), partial

  • 中文名稱：
    釀酒酵母NHX1重組蛋白
  • 貨號：
    CSB-YP312895SVG
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母NHX1重組蛋白
  • 貨號：
    CSB-EP312895SVG
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母NHX1重組蛋白
  • 貨號：
    CSB-EP312895SVG-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母NHX1重組蛋白
  • 貨號：
    CSB-BP312895SVG
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母NHX1重組蛋白
  • 貨號：
    CSB-MP312895SVG
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    NHX1
  • Uniprot No.：
  • 別名：
    NHX1; NHA2; VPL27; VPS44; YDR456W; D9461.40; Endosomal/prevacuolar sodium/hydrogen exchanger; Endosomal/prevacuolar Na(+)/H(+) exchanger; Vacuolar protein sorting-associated protein 44
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Endosomal/prevacuolar electroneutral Na(+)/H(+) exchanger which mediates intracellular sequestration of Na(+) cations, regulates vacuolar pH and contributes to osmotolerance following sudden exposure to hyperosmotic media. Contributes also to the postdiauxic/stationary phase resistance to osmotic stress and allows for the continued growth of cells until the acquired osmotolerance response can occur. Involved in hygromycin resistance probably through its influence on the electrochemical proton gradient affecting secondarily the entrance of hygromycin. Mediates pH-dependent vesicle trafficking out of the endosome. Contributes to K(+) sequestration and homeostasis.
  • 基因功能參考文獻：
    1. Data suggest that Nhx1p plays an important role in regulation of the luminal pH of various intracellular organelles, and that this regulation is critical for the protein recycling pathway as well as the endocytic pathway. PMID: 22210050
    2. Nhx1 is not required for cargo sorting or multivesicular body formation and shows synthetic phenotypes with select endosomal sorting complex required for transport mutants PMID: 21998311
    3. Nhx1p contributes to MVB formation by the recruitment of Vps27p to the endosomal membrane, possibly through Nhx1p antiporter activity. PMID: 21896492
    4. The authors conclude that Arl1p, Kha1p and Nhx1p collaborate in survival strategies at nonoptimal pH, temperatures and cation concentrations, but work independent of each other. PMID: 20659170
    5. Gene knockout of NHX1 increases sensitivity to hygromycin and acidic pH in yeast cells. PMID: 20858221
    6. the Na+K+/H+ exchanger Nhx1 regulates cellular pH to control vesicle trafficking PMID: 15635088
    7. The trafficking phenotypes of all Nhx1 mutants, including hygromycin-sensitivity and missorting of carboxypeptidase Y, were found to directly correlate with pH homoeostasis defects and could be proportionately corrected by titration with weak base. PMID: 16671892
    8. The high sequence conservation within this subfamily of yeast antiporters suggests that Nhx1p is of great importance in cell physiology PMID: 17176761

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  • 亞細胞定位：
    Endosome membrane; Multi-pass membrane protein. Prevacuolar compartment membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family
  • 數據庫鏈接：

    KEGG: sce:YDR456W

    STRING: 4932.YDR456W



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