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Recombinant Saccharomyces cerevisiae ARS-binding factor 1 (ABF1), partial

  • 中文名稱:
    Recombinant Saccharomyces cerevisiae ARS-binding factor 1(ABF1) ,partial
  • 貨號:
    CSB-YP321206SVG
  • 規格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    Recombinant Saccharomyces cerevisiae ARS-binding factor 1(ABF1) ,partial
  • 貨號:
    CSB-EP321206SVG
  • 規格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    Recombinant Saccharomyces cerevisiae ARS-binding factor 1(ABF1) ,partial
  • 貨號:
    CSB-EP321206SVG-B
  • 規格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    Recombinant Saccharomyces cerevisiae ARS-binding factor 1(ABF1) ,partial
  • 貨號:
    CSB-BP321206SVG
  • 規格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    Recombinant Saccharomyces cerevisiae ARS-binding factor 1(ABF1) ,partial
  • 貨號:
    CSB-MP321206SVG
  • 規格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    ABF1
  • Uniprot No.:
  • 別名:
    ABF1; BAF1; OBF1; REB2; SBF1; YKL112W; YKL505ARS-binding factor 1; Bidirectionally acting factor 1; DNA replication enhancer-binding protein OBF1; SFB-B
  • 種屬:
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度:
    Partial
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    General regulatory factor (GRF) that contributes to transcriptional activation of a large number of genes, as well as to DNA replication, silencing and telomere structure. Involved in the transcription activation of a subset of ribosomal protein genes. Binds the ARS-elements found in many promoters. Binds to the sequence 5'-TCN(7)ACG-3'. Influences on genome-wide nucleosome occupancy and affects chromatin structure, and probably dynamics. As a component of the global genome repair (GGR) complex, promotes global genome nucleotide excision repair (GG-NER) which removes DNA damage from nontranscribing DNA. Component of the regulatory network controlling mitotic and meiotic cell cycle progression.
  • 基因功能參考文獻:
    1. We present evidence that Abf1 recruitment upon nutritional stress, also observed for representative ribosome biogenesis genes, favours RPG transcriptional rescue upon nutrient replenishment, thus pointing to nutrient-regulated Abf1 dynamics at promoters as a novel mechanism in ribosome biogenesis control. PMID: 27016735
    2. Results show that Abf1 contributes to local regions of chromatin structure by acting at both strong and weak binding sites, at proximal promoter regions and at sites farther upstream, over a very large fraction of the yeast genome. PMID: 21081559
    3. Results compare the ability of RAP1 and ABF1 to create a region of open chromatin near and to contribute to activated transcription at promoters. PMID: 15456886
    4. DNA sequences were found that are bound by ABF1 with nearly two-magnitude higher affinity as compared to the hitherto accepted ABF1 consensus sequence. PMID: 16083878
    5. The effect on global gene expression of loss of binding of Abf1. PMID: 17158163
    6. The present study revealed numerous loci previously unknown to be under Abf1 control, and it yielded evidence for the protein's variable DNA binding pattern during mitotic growth and meiotic development. PMID: 18305101
    7. Data suggest an involvement of Abf1p transcription factor in intergenomic signaling. PMID: 18977319
    8. Mutation of the I silencer ABF1-binding site at the HMLalpha locus caused loss of ABF1 binding, which resulted in a domain of reduced global genome nucleotide excision repair efficiency on one side of the ABF1-binding site PMID: 18996839

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  • 亞細胞定位:
    Nucleus.
  • 蛋白家族:
    BAF1 family
  • 數據庫鏈接:

    KEGG: sce:YKL112W

    STRING: 4932.YKL112W



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