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  4. Recombinant Rat Glycine receptor subunit beta (Glrb), partial

Recombinant Rat Glycine receptor subunit beta (Glrb), partial

  • 中文名稱：
    大鼠Glrb重組蛋白
  • 貨號(hào)：
    CSB-YP009520RA
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    大鼠Glrb重組蛋白
  • 貨號(hào)：
    CSB-EP009520RA
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    大鼠Glrb重組蛋白
  • 貨號(hào)：
    CSB-EP009520RA-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    大鼠Glrb重組蛋白
  • 貨號(hào)：
    CSB-BP009520RA
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    大鼠Glrb重組蛋白
  • 貨號(hào)：
    CSB-MP009520RA
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Glrb
  • Uniprot No.：
  • 別名：
    GlrbGlycine receptor subunit beta; Glycine receptor 58 kDa subunit
  • 種屬：
    Rattus norvegicus (Rat)
  • 蛋白長(zhǎng)度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Glycine receptors are ligand-gated chloride channels. GLRB does not form ligand-gated ion channels by itself, but is part of heteromeric ligand-gated chloride channels. Channel opening is triggered by extracellular glycine. Heteropentameric channels composed of GLRB and GLRA1 are activated by lower glycine levels than homopentameric GLRA1. Plays an important role in the down-regulation of neuronal excitability. Contributes to the generation of inhibitory postsynaptic currents.
  • 基因功能參考文獻(xiàn)：
    1. the role of gephyrin and glycine receptor (GlyR) in vestibular compensation, was investigated. PMID: 28026001
    2. The SdpI-binding site was mapped to a proline-rich sequence of 22 amino acids within the intracellular loop of GlyRbeta. PMID: 24509844
    3. these data are consistent with roles of Vps35 and Nbea in the retrieval and post-Golgi trafficking of synaptic glycine receptor beta-subunit and possibly other neurotransmitter receptors PMID: 21821005
    4. Binding of gephyrin to the GlyR is exclusively mediated by the E domain; the binding site was mapped to one of its sub-domains; a high affinity and low affinity binding site for the GlyRbeta-loop was found on holo-gephyrin and the E domain PMID: 14976213
    5. Putative activation mechanisms were fitted to single-channel recordings at 4 glycine concentrations from rat alpha1beta receptors expressed in HEK293 cells. A substantial conformation change is caused by binding while the channel is still shut. PMID: 15574743

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  • 亞細(xì)胞定位：
    Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Cell junction, synapse. Perikaryon. Cell projection, dendrite. Cytoplasm.
  • 蛋白家族：
    Ligand-gated ion channel (TC 1.A.9) family, Glycine receptor (TC 1.A.9.3) subfamily, GLRB sub-subfamily
  • 組織特異性：
    Detected in spinal cord and brain stem (at protein level). Detected in spinal cord, cerebellum and brain cortex.
  • 數(shù)據(jù)庫鏈接：


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