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  4. Recombinant Pig Phospholipase A2, major isoenzyme (PLA2G1B)

Recombinant Pig Phospholipase A2, major isoenzyme (PLA2G1B)

  • 中文名稱：
    野豬PLA2G1B重組蛋白
  • 貨號：
    CSB-YP018090PI
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    野豬PLA2G1B重組蛋白
  • 貨號：
    CSB-EP018090PI
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    野豬PLA2G1B重組蛋白
  • 貨號：
    CSB-EP018090PI-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    野豬PLA2G1B重組蛋白
  • 貨號：
    CSB-BP018090PI
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    野豬PLA2G1B重組蛋白
  • 貨號：
    CSB-MP018090PI
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    PLA2G1B; Phospholipase A2; major isoenzyme; EC 3.1.1.4; Group IB phospholipase A2; Phosphatidylcholine 2-acylhydrolase 1B
  • 種屬：
    Sus scrofa (Pig)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區域：
    23-146
  • 氨基酸序列
    ALWQFRSM IKCAIPGSHP LMDFNNYGCY CGLGGSGTPV DELDRCCETH DNCYRDAKNL DSCKFLVDNP YTESYSYSCS NTEITCNSKN NACEAFICNC DRNAAICFSK APYNKEHKNL DTKKYC
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Secretory calcium-dependent phospholipase A2 that primarily targets dietary phospholipids in the intestinal tract. Hydrolyzes the ester bond of the fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity) with preference for phosphatidylethanolamines and phosphatidylglycerols over phosphatidylcholines. May play a role in the biosynthesis of N-acyl ethanolamines that regulate energy metabolism and inflammation in the intestinal tract. Hydrolyzes N-acyl phosphatidylethanolamines to N-acyl lysophosphatidylethanolamines, which are further cleaved by a lysophospholipase D to release N-acyl ethanolamines. May act in an autocrine and paracrine manner. Has anti-helminth activity in a process regulated by gut microbiota. Upon helminth infection of intestinal epithelia, directly affects phosphatidylethanolamine contents in the membrane of helminth larvae, likely controlling an array of phospholipid-mediated cellular processes such as membrane fusion and cell division while providing for better immune recognition, ultimately reducing larvae integrity and infectivity.
  • 基因功能參考文獻：
    1. Equilibrium unfolding of porcine pancreatic phospholipase A2 was monitored using multiple approaches. An unusually broad unfolding transition at remarkably high denaturant concentration was observed. PMID: 26117657
    2. Porcine group IB secretory phospholipase A2 (PLA2G1B) stimulates the expression and secretion of CXC chemokine ligand 8 (CXCL8) in human neutrophils without affecting other proinflammatory cytokines. PMID: 15528384
    3. Porcine pancreatic PLA(2) shows a long lag phase of several hours during which the enzyme binds to the bilayer surface, but only 5+/-3% of the lipids react before the onset of rapid hydrolysis. PMID: 17355873
    4. Results describe the structural basis for bile salt inhibition of pancreatic phospholipase A2. PMID: 17434532
    5. MMP-2/9 production is regulated by sPLA2-IB acting as a receptor ligand to activate cPLA2 PMID: 17981679
    6. Results show that the decylsulfate epitope for the successive E i (#) complexes increasingly resembles the micellar complex formed by the binding of PLA2 to preformed micelles. PMID: 18260608
    7. An increased sPLA(2) activity and a reduced inhibition may play an important role in the pathogenesis of ischemia-reperfusion injury of non-heart-beating donor liver grafts. PMID: 18468626
    8. Interfacial activation of pig pancreatic IB phospholipase A(2) (PLA2) is modeled in terms of the three discrete premicellar complexes (E(i)(#), i = 1, 2, or 3) consecutively formed by the cooperative binding of a monodisperse amphiphile to the i-face PMID: 19301847

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Phospholipase A2 family
  • 數據庫鏈接：


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