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  4. Recombinant Mouse Zinc finger protein GLIS3 (Glis3)

Recombinant Mouse Zinc finger protein GLIS3 (Glis3)

  • 中文名稱：
    小鼠Glis3重組蛋白
  • 貨號：
    CSB-YP744321MO
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    小鼠Glis3重組蛋白
  • 貨號：
    CSB-EP744321MO
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    小鼠Glis3重組蛋白
  • 貨號：
    CSB-EP744321MO-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小鼠Glis3重組蛋白
  • 貨號：
    CSB-BP744321MO
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小鼠Glis3重組蛋白
  • 貨號：
    CSB-MP744321MO
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Glis3
  • Uniprot No.：
  • 別名：
    Glis3Zinc finger protein GLIS3; GLI-similar 3
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-780
  • 氨基酸序列
    MMVQRLGPIS PPASQVSTAC KQISPSLPRA VNAANLNRPP SDTRSVILQE SLVSTTLSLT ESQSALSVKQ EWSQSYRAFP SLSSSHSSQN GTDLGDLLSL PPGTPVSGNS VSNSLPPYLF GMENSHSPYP SPRHSATRAH STRSKKRALS LSPLSDGIGI DFNTIIRTSP TSLVAYINGP RASPANLSPQ SEVYGHFLGV RGSCIPQSCA VASGQKGILV ASGGHTLPGY GEDGTLEYER MQQLEHGGLQ PGPVNNMVLQ PGLPGQDGQT ANMLKTERLE EFPASALDLP SALPLPLPPP QGPPPPYHAH PHLHHPELLP HTQSLSLAQT GLEEDGEMED SGGKHCCRWI DCSALYDQQE ELVRHIEKVH IDQRKGEDFT CFWTGCPRRY KPFNARYKLL IHMRVHSGEK PNKCTFEGCK KAFSRLENLK IHLRSHTGEK PYLCQHPGCQ KAFSNSSDRA KHQRTHLDTK PYACQIPGCT KRYTDPSSLR KHVKAHSSRE QQARKKLRSS TELHPDLLTD CLAVQPLQPA TSPGDAADHT VGHSPGPGPG PGPGAELYSA PIFASNHSTR SGTAAGAGPP PHPVSHPSPG HNVQGSPHNP SSQLPPLTAV DAGAERFAPP TPSPHHISPG RVPAPPSLLQ RAQAPHSQQP PGSLLKPYQP ETNSSFQPNG IHVHGFYGQL QTFCPPHYPD SQRTVPPSGS CSMVPSFEDC LVPTSMGQAG FDVFHRAFST HSGITVYDLP SASSSLFGES LRSGPEDPTF LQLSAVDRCP SQLSSVYTEG
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Acts as both a repressor and activator of transcription. Binds to the consensus sequence 5'-GACCACCCAC-3'.
  • 基因功能參考文獻：
    1. The observations suggest that GLIS3 promotes the gonocyte-to- spermatogonial stem cells transition and is a critical regulator of the dynamics of early postnatal spermatogenesis. PMID: 27350140
    2. Study demonstrates that Glis3 protein exhibits a temporal and cell type-specific pattern of expression during embryonic and neonatal pancreas development that is consistent with a regulatory role for Glis3 in promoting endocrine progenitor generation, regulating insulin and Ppy expression in beta and Ppy expression in beta and PP cells, respectively, and duct morphogenesis. PMID: 27270601
    3. Glis3 plays a pivotal role in the transcriptional regulation of insulin. PMID: 23927931
    4. beta cell-specific inactivation of Glis3 in adult mice downregulates insulin expression, leading to hyperglycaemia and subsequently enhanced beta cell apoptosis PMID: 23197416
    5. The results indicate that GLIS3 controls fetal islet differentiation via direct transactivation of Neurog3, a perturbation PMID: 21786021
    6. Glis3 interacts with Suppressor of Fused (SUFU) PMID: 21543335
    7. The transcription factor, GLIS3, has both repressor and activation functions. PMID: 14500813
    8. Glis3(-/-) mice had increases in the blood sugar level in the first days after birth, attributed to a decrease in Insulin mRNA level in the pancreas caused by impaired islet development and the subsequent impairment of Insulin-producing cell formation. PMID: 19481545
    9. Data show that Glis3 plays a role in cell lineage specification, particularly in the development of pancreatic beta cells, and also regulates insulin gene expression. PMID: 19805515

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  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    GLI C2H2-type zinc-finger protein family
  • 組織特異性：
    In the embryo, expressed at high levels in the kidney and testis. In the adult, expressed at high levels in the kidney and uterus and at lower levels in the brain, lung, skeletal muscle and pancreas.
  • 數據庫鏈接：


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