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  4. Recombinant Mouse Tubulin polyglutamylase TTLL7 (Ttll7), partial

Recombinant Mouse Tubulin polyglutamylase TTLL7 (Ttll7), partial

  • 中文名稱：
    小鼠Ttll7重組蛋白
  • 貨號：
    CSB-YP025264MO
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    小鼠Ttll7重組蛋白
  • 貨號：
    CSB-EP025264MO
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    小鼠Ttll7重組蛋白
  • 貨號：
    CSB-EP025264MO-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小鼠Ttll7重組蛋白
  • 貨號：
    CSB-BP025264MO
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小鼠Ttll7重組蛋白
  • 貨號：
    CSB-MP025264MO
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Ttll7
  • Uniprot No.：
  • 別名：
    Ttll7; Tubulin polyglutamylase TTLL7; EC 6.-.-.-; Tubulin--tyrosine ligase-like protein 7; mTTLL7
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Polyglutamylase which modifies tubulin, generating polyglutamate side chains of variable lengths on the gamma-carboxyl group of specific glutamate residues within the C-terminal tail of tubulin. Mediates both ATP-dependent initiation and elongation steps of the polyglutamylation reaction. Preferentially modifies the beta-tubulin tail over an alpha-tail. Competes with monoglycylase TTLL3 for modification site on beta-tubulin substrate, thereby creating an anticorrelation between glycylation and glutamylation reactions. Required for neurite growth; responsible for the strong increase in tubulin polyglutamylation during postnatal neuronal maturation.
  • 基因功能參考文獻：
    1. TTLL7, the most abundant neuronal glutamylase, adds glutamates preferentially to the beta-tubulin tail; coupled with ensemble and single-molecule biochemistry, the hybrid X-ray and cryo-electron microscopy structure of TTLL7 bound to the microtubule delineates a tripartite microtubule recognition strategy. PMID: 25959773
    2. TTLL7 is a mammalian beta-tubulin polyglutamylase required for growth of MAP2-positive neurites PMID: 16901895
    3. TTLL7 performs both the initiation and elongation of polyglutamylation on beta-tubulin; the mechanism of binding of three substrates glutamate, ATP, and tubulin to the enzyme is a random sequential pathway. PMID: 19152315
  • 亞細胞定位：
    Cell projection, cilium. Cytoplasm, cytoskeleton, cilium basal body. Cell projection, dendrite. Perikaryon.
  • 蛋白家族：
    Tubulin--tyrosine ligase family
  • 組織特異性：
    Highly expressed in brain, testis and trachea. Expressed in brain, heart, kidney, liver, lung, muscle and trachea. In the brain, highly expressed in hippocampus, thalamus, olfactory bulb and cerebellum cortex, corpus callosum and striatum.
  • 數(shù)據(jù)庫鏈接：


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