在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

Recombinant Mouse Pyruvate kinase PKM (Pkm)

In Stock
  • 中文名稱:
    小鼠Pkm重組蛋白
  • 貨號:
    CSB-EP018072MO
  • 規(guī)格:
    ¥2328
  • 圖片:
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP018072MO could indicate that this peptide derived from E.coli-expressed Mus musculus (Mouse) Pkm.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP018072MO could indicate that this peptide derived from E.coli-expressed Mus musculus (Mouse) Pkm.
  • 其他:

產(chǎn)品詳情

  • 純度:
    Greater than 85% as determined by SDS-PAGE.
  • 基因名:
  • Uniprot No.:
  • 別名:
    Pkm; Pk3; Pkm2; PykmPyruvate kinase PKM; EC 2.7.1.40; Pyruvate kinase muscle isozyme
  • 種屬:
    Mus musculus (Mouse)
  • 蛋白長度:
    Full Length
  • 來源:
    E.coli
  • 分子量:
    62.8 kDa
  • 表達(dá)區(qū)域:
    1-531aa
  • 氨基酸序列
    MPKPHSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSAPITARNTGIICTIGPASRSVEMLKEMIKSGMNVARLNFSHGTHEYHAETIKNVREATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKEKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKAADVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAIYHLQLFEELRRLAPITSDPTEAAAVGAVEASFKCCSGAIIVLTKSGRSAHQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDAVLNAWAEDVDLRVNLAMDVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPVP
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標(biāo)簽:
    N-terminal 10xHis-tagged and C-terminal Myc-tagged
  • 產(chǎn)品提供形式:
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液:
    Tris-based buffer,50% glycerol
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    3-7 business days
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA:
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能:
    Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival. In addition to its role in glycolysis, also regulates transcription. Stimulates POU5F1-mediated transcriptional activation. Promotes in a STAT1-dependent manner, the expression of the immune checkpoint protein CD274 in ARNTL/BMAL1-deficient macrophages. Also acts as a translation regulator for a subset of mRNAs, independently of its pyruvate kinase activity: associates with subpools of endoplasmic reticulum-associated ribosomes, binds directly to the mRNAs translated at the endoplasmic reticulum and promotes translation of these endoplasmic reticulum-destined mRNAs. Plays a general role in caspase independent cell death of tumor cells.
  • 基因功能參考文獻(xiàn):
    1. PKM2 promotes tumor cell exosome release via phosphorylating protein SNAP23. PMID: 28067230
    2. PKM1 interacts with A-Raf, an upstream regulator of the MEK/ERK pathway, and that this interaction contributes to MEK1 phosphorylation by A-Raf. PMID: 28743549
    3. infiltrated/activated neutrophils at wound site release pyruvate kinase M2 (PKM2) by its secretive mechanisms during early stages of wound repair; the released extracellular PKM2 facilitates early wound healing by promoting angiogenesis at wound site PMID: 26808610
    4. The results suggested that targeting PKM2 with an oncolytic adenovirus produced a strong antitumor effect. PMID: 28569774
    5. PKM2 activation may protect against DN by increasing glucose metabolic flux, inhibiting the production of toxic glucose metabolites and inducing mitochondrial biogenesis to restore mitochondrial function. PMID: 28436957
    6. our results demonstrate that PKM2 is required to support metabolic reprogramming for homocysteine-induced B cell activation and function PMID: 27903739
    7. Jmjd8 is upregulated during endothelial differentiation and regulates endothelial sprouting and metabolism by interacting with pyruvate kinase M2. PMID: 27199445
    8. this study identifies a new signaling pathway for CD43 through the regulation of alternative functions of pyruvate kinase isoform M2, favoring cell survival following activation PMID: 27606486
    9. results show that the Aha1-Hsp90-PKM2/HIF-1alpha axis mediates the induction of aromatase in Li-Fraumeni Syndrome. PMID: 27467582
    10. in addition to its role in cancer metabolism, PKM2 plays a role in controlling systemic metabolic homeostasis and inflammation, thereby preventing hepatocellular carcinoma by a non-cell-autonomous mechanism. PMID: 27125672
    11. Evidence That Does Not Support Pyruvate Kinase M2 (PKM2)-catalyzed Reaction as a Rate-limiting Step in Cancer Cell Glycolysis. PMID: 26917721
    12. PKM2 promotes cell proliferation and insulin secretion, and inhibits apoptosis in pancreatic beta cells via Wnt/CTNNB1 signaling. PMID: 26823761
    13. Using [(32)P]-phosphoenolpyruvate (PEP) we examine the direct substrates of PKM2 using recombinant enzyme and in vitro systems where PKM2 is genetically deleted. The findings argue against a role for PKM2 as a protein kinase. PMID: 26300261
    14. LPS-induced PKM2 enters into a complex with Hif-1alpha, which can directly bind to the IL-1beta promoter, an event that is inhibited by activation of PKM2. PMID: 25565206
    15. observed a shift in the enzyme pyruvate kinase from the adult M1 (PKM1) isoform to the fetal M2 (PKM2) isoform, a hallmark of the Warburg Effect, in the heart after sunitinib treatment PMID: 25735978
    16. Muller glia compensate for their unique metabolic adaptations by using lactate and aspartate from neurons as surrogates for their missing PK and AGC1. PMID: 25313047
    17. PKM1 expression promotes a metabolic state that is unable to support DNA synthesis and cell proliferation. PMID: 25482511
    18. Up-regulation of PKM2 might protect intestinal epithelial cells against apoptosis possibly through Bcl-xl in Crohn's disease. PMID: 24817408
    19. This study provides the first evidence linking natural mutations in PKM2 with cancer. PMID: 24492614
    20. Our data reveal a complex interaction between STAT6 and PPARgamma in the regulation of liver and adipose tissue lipid depot distribution and design STAT6 as a novel link between inflammatory cell metabolism and adipocyte and hepatocyte function. PMID: 23917405
    21. PKM2 activity in beta-cells is oscillatory and are consistent with pulsatile PFK1 being the mediator of slow glycolytic oscillations. PMID: 24100037
    22. These data suggest that regulation of pyruvate kinase M2 isoform activity supports the different metabolic requirements of proliferating and nonproliferating tumor cells. PMID: 24120138
    23. PKM2 expression induces changes in type 1 fibers associated with muscle atrophy and muscle weakness in myotonic dystrophy type 1. PMID: 23901116
    24. PPARgamma contributes to PKM2 and HK2 expression in fatty liver PMID: 22334075
    25. Components of the mTOR/HIF1alpha/Myc-hnRNPs/PKM2 glycolysis signaling network could be targeted for the treatment of cancer caused by an aberrant RTK/PI3K/AKT/mTOR signaling pathway PMID: 21325052
    26. histidine-phosphorylated PGAM1 correlated with expression of PKM2 in cancer cell lines; decreased pyruvate kinase activity in PKM2-expressing cells allows PEP-dependent histidine phosphorylation of PGAM1 and may provide an alternate glycolytic pathway PMID: 20847263
    27. splicing repressors hnRNP A1 and A2, as well as the polypyrimidine-tract-binding protein PTB, contribute to control of pyruvate kinase isoform M1 and M2 expression PMID: 20133837
    28. Findings suggest that tyrosine phosphorylation regulates PKM2 to provide a metabolic advantage to tumor cells, thereby promoting tumor growth. PMID: 19920251
    29. study showed IL-3-induced nuclear translocation of the M2 isoform of pyruvate kinase (M2-PK) and examined its significance in cell proliferation [pyruvate kinase M2 isoform] PMID: 17446165
    30. results demonstrate that M2 expression is necessary for aerobic glycolysis and that this metabolic phenotype provides a selective growth advantage for tumour cells in vivo PMID: 18337823
    31. These findings suggest that M2 pyruvate kinase is a metabolic sensor which regulates cell proliferation, cell growth and apoptotic cell death in a glucose supply-dependent manner. PMID: 19563799

    顯示更多

    收起更多

  • 亞細(xì)胞定位:
    Cytoplasm. Nucleus.
  • 蛋白家族:
    Pyruvate kinase family
  • 組織特異性:
    Embryonic stem cells and embryonal carcinoma cells.
  • 數(shù)據(jù)庫鏈接:


主站蜘蛛池模板: 青青爽无码视频在线观看| 小12萝裸体自慰出白浆| 国产 高潮 抽搐 正在播放| 亚洲精品av一二三区无码| 亚洲精品无码鲁网中文电影| 色欲国产麻豆一精品一av一免费| 狠狠的干性视频| 国产婷婷一区二区三区| 亚洲国产专区校园欧美| 鲁一鲁一鲁一鲁一曰综合网| 少妇高潮喷水惨叫久久久久电影| 欧美黑人又粗又大又爽免费| 久久国产色av免费看| 狠狠躁夜夜躁人人躁婷婷| 日韩成人无码v清免费| 一区二三区在线 | 中国| 丰满少妇69激情啪啪无| 午夜理论片yy44880影院| 日韩精品无码中文字幕一区二区 | 国产一区二区三区内射高清| 亚洲欧美日韩在线不卡| 亚洲人成色777777精品音频| 卡1卡2卡3精品接入口| 国产成av人片在线观看无码| 伊人久久大香线蕉av色婷婷色| 国产精品成人免费一区久久羞羞| 国产av亚洲精品久久久久久| 精品精品国产欧美在线小说区| 欧美成人a在线网站| 少妇久久久久久被弄到高潮| 国产无遮挡a片无码免费| 理论片午午伦夜理片影院| 久久综合狠狠综合久久激情| 亚洲 高清 成人 动漫| 国产人与禽zoz0性伦| 无遮无挡爽爽免费视频| 日本免费高清线视频免费| 亚洲国产婷婷六月丁香| 午夜美女裸体福利视频| 久久一卡二卡三卡四卡| 日韩精品人成在线播放|