Recombinant Mouse Metallothionein-3 (Mt3)

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中文名稱：

小鼠Mt3重組蛋白
貨號：

CSB-EP015122MO
規(guī)格：

￥1836
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情

產(chǎn)品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
基因名：

Mt3
Uniprot No.：

P28184
別名：

Growth inhibitory factor Short name: GIF
種屬：

Mus musculus (Mouse)
蛋白長度：

Full Length
來源：

E.coli
分子量：

14.5 kDa
表達區(qū)域：

1-68aa
氨基酸序列

MDPETCPCPTGGSCTCSDKCKCKGCKCTNCKKSCCSCCPAGCEKCAKDCVCKGEEGAKAEAEKCSCCQ
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 10xHis-tagged and C-terminal Myc-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

Binds heavy metals. Contains three zinc and three copper atoms per polypeptide chain and only a negligible amount of cadmium. Inhibits survival and neurite formation of cortical neurons in vitro.
基因功能參考文獻：
1. the absence of Mt3 reduces Abeta uptake in astrocytes through an abnormality in actin polymerization. PMID: 26637294
2. Circadian time and lighting could be involved in the regulation of the expression of melatonin receptors MTNR3 and Rorc. PMID: 25189895
3. Mt3 may act through PDE3a to play a key role in Zinc dyshomeostasis and cell death in streptozotocin-treated islets. PMID: 25054451
4. Zn released from MT3 may contribute to VEGF induction. PMID: 23962989
5. The entire MT3 peptide shows a high capacity to bind Cu(+) , provided that this occurs in a nonoxidative milieux. This reflects a peculiar property of this MT isoform, which senses different Cu contents in the environment in which it is synthesized. PMID: 24479872
6. MT-III can help protect against light-induced retinal damage compared to MT-I/II. Some of these effects may be exerted by its antioxidative potency. PMID: 23132798
7. MT-3 is able to alter the Tg2576 phenotype in several aspects such as mortality and behavior in a gender-dependent manner. PMID: 22722772
8. ZnMt3 in cultured astrocytes may be a normal component of c-Abl activation in EGF receptor signaling PMID: 21900236
9. Metallothionein-III null mice show attenuation of cadmium-induced severe testicular toxicity, suggesting that lack of MT-III contributes to protection of testis from cadmium. PMID: 20851133
10. results indicate that MT3 may play a key role in normal lysosomal function in cultured astrocytes PMID: 20544854
11. findings indicate that abnormalities of psychological behavior were observed in the MT-3 knock-out mice PMID: 19799968
12. engineered protein has neuroinhibitory activity when introduced into metallothionein-1 PMID: 12130647
13. a correct MTs homeostasis is pivotal for brain-endocrine-immune response in order to reach successful ageing. PMID: 12714242
14. Metallothionein 3 [Mt3]-null mice had increased expression of neurotrophins as well as GAP-43 following cryoinjury. Thus MT-III does not affect the inflammatory response, but it may influence neuronal regeneration during recovery PMID: 12758064
15. MT-III isoform, which has a limited tissue distribution, especially in the central nervous system, seems to be implicated in tissue repair and/or protection against critical tissue injury PMID: 17435258
16. These findings indicate that neuronal damage was aggravated by reperfusion injury in the MT-III KO mice compared with the wild-type mice, suggesting that MT-III plays anti-oxidative and neuroprotective roles in transient cerebral ischemia. PMID: 19635467
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蛋白家族：

Metallothionein superfamily, Type 1 family
組織特異性：

Brain.
數(shù)據(jù)庫鏈接：

KEGG: mmu:17751

STRING: 10090.ENSMUSP00000034211

UniGene: Mm.2064