Recombinant Mouse Gamma-interferon-inducible lysosomal thiol reductase (Ifi30)

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中文名稱：

Recombinant Mouse Gamma-interferon-inducible lysosomal thiol reductase (Ifi30)
貨號：

CSB-EP872152MO
規(guī)格：

￥1536
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
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靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
生物活性：

Not Test
基因名：

Ifi30
Uniprot No.：

Q9ESY9
別名：

Gamma-interferon-inducible protein IP-30;Lysosomal thiol reductase IP30
種屬：

Mus musculus (Mouse)
蛋白長度：

Full Length of Mature Protein
來源：

E.coli
分子量：

27.0 kDa
表達區(qū)域：

55-230aa
氨基酸序列

PSPPVRVSLYYESLCGACRYFLVRDLFPTWLMVMEIMNITLVPYGNAQERNVSGTWEFTCQHGELECRLNMVEACLLDKLEKEAAFLTIVCMEEMDDMEKKLGPCLQVYAPEVSPESIMECATGKRGTQLMHENAQLTDALHPPHEYVPWVLVNEKPLKDPSELLSIVCQLYQGTE
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

C-terminal 6xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Lysosomal thiol reductase that can reduce protein disulfide bonds. May facilitate the complete unfolding of proteins destined for lysosomal degradation. Plays an important role in antigen processing. Facilitates the generation of MHC class II-restricted epitodes from disulfide bond-containing antigen by the endocytic reduction of disulfide bonds. Facilitates also MHC class I-restricted recognition of exogenous antigens containing disulfide bonds by CD8+ T-cells or crosspresentation.
基因功能參考文獻：
1. GILT has a role in maintaining cysteine cathepsin proteolytic efficiency in phagosomes PMID: 25253686
2. These results show that wild-type mouse fibroblasts are relatively resistant and GILT(-/-)mouse fibroblasts are susceptible to Dengue virus 2 entry and replication. PMID: 23642353
3. Data indicate that in the absence of Gamma-interferon-inducible lysosomal thiol reductase (GIL, CD4-positive T cell responses to major house dust mite allergen Der p 1 are significantly reduced. PMID: 23326313
4. GILT has a critical role in regulating CD4(+) T-cell tolerance to an endogenous skin-restricted antigen relevant to controlling autoimmunity and generating effective immunotherapy for melanoma. PMID: 21833020
5. gene knock-outs increase oxidative stress and autophagy, which is regulated by altering glutathione synthesis and oxidation PMID: 21640818
6. GILT has limited yet unexpected effect on self-peptide species presented by major histocompatibility complex class II antigens. PMID: 20485683
7. study shows GILT facilitates MHC class I-restricted recognition of exogenous antigens containing disulfide bonds by CD8+ T cells or cross-presentation;initiation of gB-specific CD8+ T cell response during HSV-1 infection or cross-priming is GILT-dependent PMID: 20538950
8. the thiol reductase activity of GILT is its essential function in MHC class II-restricted Ag processing PMID: 17142755
9. findings show that endogenously produced peptide-IgG is processed by transduced splenic B cells in the lysosomes/endosomes in a GILT-dependent manner, and this endocytic processing pathway is essential for generating tolerogenic B cells PMID: 18606668
10. Replication of Listeria monocytogenes in GILT-negative macrophages, or macrophages expressing an enzymatically inactive GILT mutant, is impaired because of delayed escape from the phagosome PMID: 18815593
11. Expression of GILT in T-lymphocytes increases as a function of maturity, thereby reducing sensitivity of T-cells and consequences of autoimmune responses; a role of GILT in the immune system may be in reducing the impact of T-cell autoreactivity. PMID: 19124716
12. GILT facilitated the processing and presentation to antigen-specific T cells of protein antigens containing disulfide bonds. PMID: 11701933
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亞細胞定位：

Secreted. Lysosome.
蛋白家族：

GILT family
數據庫鏈接：

KEGG: mmu:65972

STRING: 10090.ENSMUSP00000034299

UniGene: Mm.30241