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Recombinant Macaca fascicularis Angiotensin-converting enzyme (ACE2), partial (Active)

In Stock
  • 貨號:
    CSB-MP3414MOV
  • 規格:
    ¥1140
  • 圖片:
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Activity
      Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD (CSB-MP3324GMY1b1) at 5 μg/ml can bind Cynomolgus-ACE2, the EC50 is 5.638-9.496 ng/ml. Biological Activity Assay
    • The purity of ACE2 was greater than 95% as determined by SEC-HPLC
    • The purity of ACE2 was greater than 95% as determined by SEC-HPLC
  • 其他:

產品詳情

  • 純度:
    Greater than 90% as determined by SDS-PAGE.
    Greater than 90% as determined by SEC-HPLC.
  • 內毒素:
    Less than 1.0 EU/ug as determined by LAL method.
  • 生物活性:
    ①Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD (CSB-MP3324GMY1b1) at 5 μg/ml can bind Cynomolgus-ACE2, the EC50 is 5.638-9.496 ng/ml.
  • 基因名:
  • Uniprot No.:
  • 分子結構:
  • 種屬:
    Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
  • 蛋白長度:
    Partial
  • 來源:
    Mammalian cell
  • 分子量:
    112.7 kDa
  • 表達區域:
    18-741aa
  • 氨基酸序列
    QSTIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGEKWSAFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPNNPQECLLLDPGLNEIMEKSLDYNERLWAWEGWRSEVGKQLRPLYEEYVVLKNEMARANHYKDYGDYWRGNYEVNGVDGYDYNRDQLIEDVERTFEEIKPLYEHLHAYVRAKLMNAYPSYISPTGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVNQAWNAQRIFKEAEKFFVSVGLPNMTQGFWENSMLTDPGNVQKVVCHPTAWDLGKGDFRIIMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLLNMLKLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSIKVRISLKSALGDKAYEWNDNEMYLFRSSVAYAMRTYFLEIKHQTILFGEEDVRVADLKPRISFNFYVTAPKNVSDIIPRTEVEEAIRISRSRINDAFRLNDNSLEFLGIQTTLAPPYQSPVTT
  • 蛋白標簽:
    C-terminal hFc1-tagged
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液:
    Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    3-7 business days
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 產品描述:

    CUSABIO cloned the coding sequence of Macaca fascicularis angiotensin-converting enzyme (ACE2) (amino acids 18-741) to produce a fusion recombinant protein with the Fc portion of the human IgG1 tag in mammalian cells. This recombinant Macaca fascicularis ACE2 protein is an active protein, whose bioactivity has been measured by its binding with SARS-CoV-2-S1-RBD (EC50 is 5.638-9.496 ng/ml). It is characterized by high purity (>94%) and low endotoxin (<1.0 EU/ug protein). Due to the glycosylation, it has an apparent molecular mass of 114 kDa on the gel. It is in stock now.

    High expression of ACE2 is restricted to the endothelial cells of the arteries, arterioles, and venules of the heart and kidney. ACE2 is involved in regulating blood pressure and keeping blood pressure homeostasis. ACE2 binding to the SARS-CoV-2 S protein facilitates the viral entry into host cells, resulting in the lethal viral infection.

  • Datasheet & COA:
    Please contact us to get it.

產品評價



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