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  4. Recombinant Mediator of RNA polymerase II transcription subunit 15 (mdt-15), partial

Recombinant Mediator of RNA polymerase II transcription subunit 15 (mdt-15), partial

  • 中文名稱：
    秀麗隱桿線蟲mdt-15重組蛋白
  • 貨號(hào)：
    CSB-YP632431CXY
  • 說明書：
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲mdt-15重組蛋白
  • 貨號(hào)：
    CSB-EP632431CXY
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲mdt-15重組蛋白
  • 貨號(hào)：
    CSB-EP632431CXY-B
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲mdt-15重組蛋白
  • 貨號(hào)：
    CSB-BP632431CXY
  • 說明書：
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲mdt-15重組蛋白
  • 貨號(hào)：
    CSB-MP632431CXY
  • 說明書：
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    mdt-15
  • Uniprot No.：
  • 別名：
    mdt-15; R12B2.5Mediator of RNA polymerase II transcription subunit 15; Mediator complex subunit 15
  • 種屬：
    Caenorhabditis elegans
  • 蛋白長度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for regulated expression of genes controlling fatty acid desaturation by factors including sbp-1 and nhr-49.
  • 基因功能參考文獻(xiàn)：
    1. Prolongation of survival under glucose exposure by quercetin is dependent on SIR-2.1, DAF-12, and MDT-15 PMID: 24407905
    2. MDT-15/MED15 is required for the xenobiotic-induced expression of p38 MAP kinase PMK-1-dependent immune genes and protection from Pseudomonas aeruginosa infection PMID: 24875643
    3. These findings reveal novel conserved players in the oxidative stress response and suggest a broad cytoprotective role for MDT-15. PMID: 23957350
    4. Mdt-15 worms exhibit disturbed endoplasmic reticulum (ER) homeostasis, as indicated by a constitutively activated endoplasmic reticulum unfolded protein response. PMID: 24843123
    5. Certain compounds have increased toxicity when combined with a dietary restriction regimen through increased metabolic activation through the MDT-15 transcription factor. PMID: 22132200
    6. A single subunit of the Mediator complex, MDT-15, integrates the activities of several distinct regulatory factors to coordinate metabolic and hormonal regulation of FA metabolism. PMID: 16651656
    7. the C. elegans ARC105 homologue MDT-15 is required for fatty acid homeostasis, and both SBP-1 and MDT-15 control transcription of genes governing desaturation of stearic acid to oleic acid PMID: 16799563
    8. propose a model whereby MDT-15 integrates several transcriptional regulatory pathways to monitor both the availability and quality of ingested materials, including nutrients and xenobiotic compounds PMID: 18454197

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  • 亞細(xì)胞定位：
    Nucleus.
  • 蛋白家族：
    Mediator complex subunit 15 family
  • 組織特異性：
    Expressed in the intestine and head neurons.
  • 數(shù)據(jù)庫鏈接：

    KEGG: cel:CELE_R12B2.5

    STRING: 6239.R12B2.5a.1

    UniGene: Cel.38778



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