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  4. Recombinant Human tRNA-splicing endonuclease subunit Sen2 (TSEN2)

Recombinant Human tRNA-splicing endonuclease subunit Sen2 (TSEN2)

  • 中文名稱：
    人TSEN2重組蛋白
  • 貨號：
    CSB-YP850858HU
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    人TSEN2重組蛋白
  • 貨號：
    CSB-EP850858HU
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    人TSEN2重組蛋白
  • 貨號：
    CSB-EP850858HU-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    人TSEN2重組蛋白
  • 貨號：
    CSB-BP850858HU
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    人TSEN2重組蛋白
  • 貨號：
    CSB-MP850858HU
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    TSEN2
  • Uniprot No.：
  • 別名：
    HsSen2; MGC2776; MGC4440; SEN2; SEN2_HUMAN; SEN2L ; tRNA intron endonuclease Sen2; tRNA splicing endonuclease 2 homolog (SEN2; S. cerevisiae); tRNA splicing endonuclease subunit Sen2; tRNA-intron endonuclease sen2; tRNA-splicing endonuclease subunit Sen2; TSEN 2; TSEN2
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-465
  • 氨基酸序列
    MAEAVFHAPK RKRRVYETYE SPLPIPFGQD HGPLKEFKIF RAEMINNNVI VRNAEDIEQL YGKGYFGKGI LSRSRPSFTI SDPKLVAKWK DMKTNMPIIT SKRYQHSVEW AAELMRRQGQ DESTVRRILK DYTKPLEHPP VKRNEEAQVH DKLNSGMVSN MEGTAGGERP SVVNGDSGKS GGVGDPREPL GCLQEGSGCH PTTESFEKSV REDASPLPHV CCCKQDALIL QRGLHHEDGS QHIGLLHPGD RGPDHEYVLV EEAECAMSER EAAPNEELVQ RNRLICRRNP YRIFEYLQLS LEEAFFLVYA LGCLSIYYEK EPLTIVKLWK AFTVVQPTFR TTYMAYHYFR SKGWVPKVGL KYGTDLLLYR KGPPFYHASY SVIIELVDDH FEGSLRRPLS WKSLAALSRV SVNVSKELML CYLIKPSTMT DKEMESPECM KRIKVQEVIL SRWVSSRERS DQDDL
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. Isoform 1 probably carries the active site for 5'-splice site cleavage. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events. Isoform 2 is responsible for processing a yet unknown RNA substrate. The complex containing isoform 2 is not able to cleave pre-tRNAs properly, although it retains endonucleolytic activity.
  • 相關疾病：
    Pontocerebellar hypoplasia 2B (PCH2B)
  • 亞細胞定位：
    Nucleus. Nucleus, nucleolus. Note=May be transiently localized in the nucleolus.
  • 蛋白家族：
    TRNA-intron endonuclease family
  • 組織特異性：
    Isoform 1 and isoform 2 are widely expressed at very low level.
  • 數據庫鏈接：

    HGNC: 28422

    OMIM: 608753

    KEGG: hsa:80746

    STRING: 9606.ENSP00000284995

    UniGene: Hs.335550



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