Recombinant Human Tubulin beta chain (TUBB)

^{In Stock}

中文名稱：

人TUBB重組蛋白
貨號：

CSB-EP025318HU
規格：

￥1536
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
基因名：

TUBB
Uniprot No.：

P07437
別名：

Tubulin beta-5 chain
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length
來源：

E.coli
分子量：

50.6 kDa
表達區域：

1-444aa
氨基酸序列

MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQVFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAVTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATAEEEEDFGEEAEEEA
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

C-terminal 6xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
基因功能參考文獻：
1. human tumor cells can acquire spontaneous mutations in beta1-tubulin that cause resistance to paclitaxel PMID: 20103599
2. Roles of tubulin beta 1,3 residues Ala428 and Thr429 in microtubule formation in vivo. PMID: 19074767
3. TUBB exon 4 mutations and mismatch repair defects do not play a significant role in paclitaxel/cisplatin resistance PMID: 16095531
4. Mutations in either TUBB or MAPRE2 cause circumferential skin creases Kunze type. PMID: 26637975
5. the dipole moments of each tubulin isotype may influence their functional characteristics within the cell, resulting in differences for MT assembly kinetics and stability PMID: 16941085
6. Leucine point mutations viz. L215H, L217R, and L225M were reported for paclitaxel resistance in various cancers. In the current study, molecular mechanism of these resistance causing mutations in TUBB1 was explored using molecular docking, molecular dynamics simulation, binding energy estimation, free energy decomposition, principle component analysis and free energy landscape methods. PMID: 27233604
7. Data suggest a looser binding of the ligand in tubulin mutants. PMID: 26081685
8. Data show that tubulin phosphorylation and acetylation play important roles in the control of microtubule assembly and stability. PMID: 26165356
9. Data suggest that, while lacking a stable structure, NFL-TBS.40-63 peptide (a peptide derived from light neurofilament protein) preferentially binds on a specific single site located near C-terminal end of beta-tubulin. PMID: 26016807
10. Citrullination of TUBB is associated with neoplasms. PMID: 24099319
11. mechanism of binding and stabilization of microtubules in mammalian cells can be effectively modeled in yeast and also having the advantage of lacking any beta-tubulin isotypes that can complicate interpretation of experiments in mammalian cells. PMID: 24161989
12. Studies suggest that tubulin-interactive agents have the potential to play a significant role in the fight against cancer. PMID: 23818224
13. results provide insight into the functional repertoire of the tubulin gene family, specifically implicating TUBB5 in embryonic neurogenesis and microcephaly PMID: 23246003
14. TQ induced a concentration- and time-dependent degradation of alpha/beta tubulin in both cancer cell types. PMID: 21881916
15. Allele frequencies between a group of 191 ITP patients & controls showed no direct aetiological role for SNP (R307H), but it was associated with immunomodulatory treatment failure. PMID: 23157319
16. Data show that knockdown of S100P led to downregulation of thioredoxin 1 and beta-tubulin and upregulation of RhoGDIA, all potential therapeutic targets in cancer. PMID: 21327297
17. This is the first cell-based evidence to support a beta-tubulin-binding site for peloruside A and laulimalide. PMID: 21653684
18. Mutational analysis of the class I beta-tubulin gene in human breast cancer PMID: 12209587
19. the influence of beta-tubulin mutations in paclitaxel resistance in advanced non-small cell lung cancer PMID: 12826311
20. Review of studies comparing beta-tubulin mutations with antitubulin drug resistance. Throws doubts on earlier such correlation because of the existence of many pseudogenes for beta-tubulin. PMID: 15003198
21. These data indicate that phosphorylation of tubulin by Cdk1 could be involved in the regulation of microtubule dynamics during mitosis. PMID: 16371510
22. cDNA subtraction revealed increased expression of alpha3-tubulin in the taxol-resistant cell line. PMID: 16380805
23. Nuclear matrix proteins such as mutant Pyst1 and nucleophosmin 1 were downregulated, whereas eIF6 and beta-tubulin were upregulated during cell differentiation in hepatocarcinoma cells. PMID: 17569113
24. Results suggest that glutamate198 in beta-tubulin is a critical determinant for microtubule stability and Taxol resistance. PMID: 17869412
25. This protein has been found differentially expressed in the Wernicke's Area from patients with schizophrenia. PMID: 19405953
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相關疾病：

Cortical dysplasia, complex, with other brain malformations 6 (CDCBM6); Skin creases, congenital symmetric circumferential, 1 (CSCSC1)
亞細胞定位：

Cytoplasm, cytoskeleton.
蛋白家族：

Tubulin family
組織特異性：

Ubiquitously expressed with highest levels in spleen, thymus and immature brain.
數據庫鏈接：

HGNC: 20778

OMIM: 156610

KEGG: hsa:203068

STRING: 9606.ENSP00000339001

UniGene: Hs.636480