Recombinant Human Receptor-type tyrosine-protein phosphatase-like N (PTPRN), partial

中文名稱：

人PTPRN重組蛋白
貨號：

CSB-EP613703HU
規格：

￥1344
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
基因名：

PTPRN
Uniprot No.：

Q16849
別名：

(R-PTP-N)(Islet cell antigen 512)(ICA 512)(Islet cell autoantigen 3)(PTP IA-2)
種屬：

Homo sapiens (Human)
蛋白長度：

Extracellular Domain
來源：

E.coli
分子量：

61.3 kDa
表達區域：

35-575aa
氨基酸序列

VSAHGCLFDRRLCSHLEVCIQDGLFGQCQVGVGQARPLLQVTSPVLQRLQGVLRQLMSQGLSWHDDLTQYVISQEMERIPRLRPPEPRPRDRSGLAPKRPGPAGELLLQDIPTGSAPAAQHRLPQPPVGKGGAGASSSLSPLQAELLPPLLEHLLLPPQPPHPSLSYEPALLQPYLFHQFGSRDGSRVSEGSPGMVSVGPLPKAEAPALFSRTASKGIFGDHPGHSYGDLPGPSPAQLFQDSGLLYLAQELPAPSRARVPRLPEQGSSSRAEDSPEGYEKEGLGDRGEKPASPAVQPDAALQRLAAVLAGYGVELRQLTPEQLSTLLTLLQLLPKGAGRNPGGVVNVGADIKKTMEGPVEGRDTAELPARTSPMPGHPTASPTSSEVQQVPSPVSSEPPKAARPPVTPVLLEKKSPLGQSQPTVAGQPSARPAAEEYGYIVTDQKPLSLAAGVKLLEILAEHVHMSSGSFINISVVGPALTFRIRHNEQNLSLADVTQQAGLVKSELEAQTGLQILQTGVGQREEAAAVLPQTAHSTSPMR
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Plays a role in vesicle-mediated secretory processes. Required for normal accumulation of secretory vesicles in hippocampus, pituitary and pancreatic islets. Required for the accumulation of normal levels of insulin-containing vesicles and preventing their degradation. Plays a role in insulin secretion in response to glucose stimuli. Required for normal accumulation of the neurotransmitters norepinephrine, dopamine and serotonin in the brain. In females, but not in males, required for normal accumulation and secretion of pituitary hormones, such as luteinizing hormone (LH) and follicle-stimulating hormone (FSH). Required to maintain normal levels of renin expression and renin release. Seems to lack intrinsic enzyme activity. May regulate catalytic active protein-tyrosine phosphatases such as PTPRA through dimerization.; ICA512-TMF regulates dynamics and exocytosis of insulin secretory granules (SGs); binding of ICA512-TMF to SNTB2/beta-2-syntrophin is proposed to restrain SGs mobility and exocytosis by tethering them to the actin cytoskeleton depending on UTRN; the function is inhibited by cytoplasmic ICA512-CFF dimerizing with ICA512-TMF and displacing SNTB2.; ICA512-CCF translocated to the nucleus promotes expression of insulin and other granule-related genes; the function implicates binding to and regulating activity of STAT5B probably by preventing its dephosphorylation and potentially by inducing its sumoylation by recruiting PIAS4. Enhances pancreatic beta-cell proliferation by converging with signaling by STAT5B and STAT3. ICA512-CCF located in the cytoplasm regulates dynamics and exocytosis of insulin secretory granules (SGs) by dimerizing with ICA512-TMF and displacing SNTB2 thus enhancing SGs mobility and exocytosis.
基因功能參考文獻：
1. E. coli GI724 strain emerged as a handy source of recombinant IA-2ic, achieving high levels of expression as a thioredoxin fusion protein, adequately validated and applicable to the development of innovative and cost-effective immunoassays for IA-2A detection in most laboratories. PMID: 27881117
2. Identification of amino acids contributing to distinct epitopes on IA-2, with both HLA-DR and HLA-DQ alleles influencing epitope specificity. PMID: 26564179
3. RESP18HD is required for efficient sorting of ICA512 to secretory granules: RESP18HD is a key determinant for ICA512 granule targeting. PMID: 26836020
4. The binding of CREB to the promoter region, -216 to +115, enhanced IA-2 transcription by more than fivefold. PMID: 25528004
5. Kaplan-Meier survival curves estimated a worst pancreas graft survival for patients with positive IA-2 antibodies versus those patients with negative auto-antibodies and GAD65+ auto-antibodies after simultaneous pancreas kidney transplantation. PMID: 25645784
6. Humoral responses to islet antigen-2 and zinc transporter 8 are attenuated in patients carrying HLA-A*24 alleles at the onset of type 1 diabetes. PMID: 23396399
7. identification of cysteines that are critical for IA-2A binding of autoantibodies in patients with newly diagnosed type 1 diabetes PMID: 22966073
8. the IA-2A autoantibody response in children is intense with rapid maturation against immunogenic epitopes and a strong association with diabetes development PMID: 23110943
9. Letter: report lack of anti-IA2 autoantibodies in primary antiphospholipid syndrome. PMID: 21442164
10. analysis of protein-protein interactions in crystals of the human receptor-type protein tyrosine phosphatase ICA512 ectodomain PMID: 21935384
11. ZnT8A, GADA and IA-2A are autoantibodies that may have a role in successful pancreas graft survival PMID: 21792090
12. In families with type 1 diabetes, there was a female predominance and more family history of associated autoimmune diseases (AAIDs) in the group with AAIDs, and less frequent anti-IA-2 antibodies PMID: 21744463
13. Longer survival was associated with hypomethylation at specific CpG sites (e.g. GREB1, TGIF and TOB1) and hypermethylation in other genes (e.g. TMCO5, PTPRN and GUCY2C). PMID: 21577013
14. Studies indicate that T1DM can be detected by determining four autoantibodies, namely those antibodies against insulin, glutamic acid decarboxylase 65, insulinoma antigen (IA)-2 (ICA512) and the zinc transporter ZnT8. PMID: 21073664
15. Risk of autoantibody seroconversion among children followed in DPT-1 is age dependent PMID: 21270193
16. Data show that significantly increased expression of MCP-1, IL-2 and PTPR-N was observed in Tourette's syndrome cases. PMID: 20193755
17. T cells stimulated in vitro by the IA-2 epitope express IFN-gamma when restimulated by the similar rotavirus VP7 peptide. PMID: 20083660
18. Report a chimeric assay providing an efficient and economical technique to screen for islet autoantibodies reacting with IA-2 and ZnT8 in type 1 diabetes mellitus. PMID: 20035758
19. ICA512(IA-2) epitope specific assays distinguish transient from diabetes associated autoantibodies. PMID: 11908951
20. Autoantibodies to IA-2 in type 1 diabetes: measurements with a new enzyme-linked immunosorbent assay. PMID: 12021115
21. BCG vaccination and GAD65 and IA-2 autoantibodies in autoimmune diabetes in southern India. PMID: 12021127
22. findings indicate that glutamine at position 862, and residues 876-880 of the WPD loop of IA-2 are important for several of the IA-2 specific PTP domain epitopes PMID: 14624760
23. The islet cell autoantigen 512 (ICA512)/IA-2 is a receptor tyrosine phosphatase-like protein associated with the insulin secretory granules (SGs) of pancreatic beta-cells. PMID: 15596545
24. Measuring IA-2A is useful for the diagnosis and prognosis of type 1 diabetes in Japanese. PMID: 15620435
25. IA-2 is an important regulator of dense core vesicle number and glucose-induced and basal insulin secretion in beta cells. PMID: 15939893
26. identification of proteins that interact with IA-2, the IA-2 interactome; the IA-2 interactome based on pull-down experiments, currently consists of 12 proteins; identification of these interacting proteins provides clues as to how IA-2 functions PMID: 16273344
27. Autoantibodies in type 1 diabetes for this protein provide a model for other autoimmune diseases. PMID: 18373080
28. HLA class II alleles strongly influence the prevalence of IA-2A in type 1 diabetes. PMID: 18504544
29. Inexpensive, nonradioactive method for the detection of autoantibodies cell-prepared PTPRN (also known as IA-2) in diabetics. PMID: 18535195
30. The study identifies a region of IA-2 frequently recognised by antibodies in Type 1 diabetes and demonstrates that these responses are associated with T-cells secreting IL-10 in response to a neighbouring determinant. PMID: 19447008
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相關疾?。?/div>
Autoantigen in insulin-dependent diabetes mellitus (IDDM).
亞細胞定位：

Membrane; Single-pass type I membrane protein. Cytoplasmic vesicle, secretory vesicle membrane; Single-pass type I membrane protein. Perikaryon. Cell projection, axon. Cell junction, synapse. Cell membrane; Single-pass type I membrane protein. Endosome.; [ICA512-transmembrane fragment]: Cytoplasmic vesicle, secretory vesicle membrane.; [ICA512-cleaved cytosolic fragment]: Nucleus.
蛋白家族：

Protein-tyrosine phosphatase family, Receptor class 8 subfamily
組織特異性：

Expression is restricted to neuroendocrine cells. Found in pancreas, brain and pituitary.
數據庫鏈接：

HGNC: 9676

OMIM: 601773

KEGG: hsa:5798

STRING: 9606.ENSP00000295718

UniGene: Hs.89655