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Recombinant Human Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15 (DHX15)

  • 中文名稱:
    人DHX15重組蛋白
  • 貨號:
    CSB-YP006870HU
  • 規格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    人DHX15重組蛋白
  • 貨號:
    CSB-EP006870HU
  • 規格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    人DHX15重組蛋白
  • 貨號:
    CSB-EP006870HU-B
  • 規格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    人DHX15重組蛋白
  • 貨號:
    CSB-BP006870HU
  • 規格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    人DHX15重組蛋白
  • 貨號:
    CSB-MP006870HU
  • 規格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    DHX15
  • Uniprot No.:
  • 別名:
    ATP dependent RNA helicase #46; ATP-dependent RNA helicase #46; DBP1; DDX15; DEADH box 15; DEAH (Asp Glu Ala His) box polypeptide 15; DEAH box polypeptide 15; DEAH box protein 15; DHX15; DHX15_HUMAN; HRH2; Pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15; PRP43; PrPp43p; RNA helicase 2
  • 種屬:
    Homo sapiens (Human)
  • 蛋白長度:
    Full length protein
  • 表達區域:
    1-795
  • 氨基酸序列
    MSKRHRLDLG EDYPSGKKRA GTDGKDRDRD RDREDRSKDR DRERDRGDRE REREKEKEKE LRASTNAMLI SAGLPPLKAS HSAHSTHSAH STHSTHSAHS THAGHAGHTS LPQCINPFTN LPHTPRYYDI LKKRLQLPVW EYKDRFTDIL VRHQSFVLVG ETGSGKTTQI PQWCVEYMRS LPGPKRGVAC TQPRRVAAMS VAQRVADEMD VMLGQEVGYS IRFEDCSSAK TILKYMTDGM LLREAMNDPL LERYGVIILD EAHERTLATD ILMGVLKEVV RQRSDLKVIV MSATLDAGKF QIYFDNCPLL TIPGRTHPVE IFYTPEPERD YLEAAIRTVI QIHMCEEEEG DLLLFLTGQE EIDEACKRIK REVDDLGPEV GDIKIIPLYS TLPPQQQQRI FEPPPPKKQN GAIGRKVVVS TNIAETSLTI DGVVFVIDPG FAKQKVYNPR IRVESLLVTA ISKASAQQRA GRAGRTRPGK CFRLYTEKAY KTEMQDNTYP EILRSNLGSV VLQLKKLGID DLVHFDFMDP PAPETLMRAL ELLNYLAALN DDGDLTELGS MMAEFPLDPQ LAKMVIASCD YNCSNEVLSI TAMLSVPQCF VRPTEAKKAA DEAKMRFAHI DGDHLTLLNV YHAFKQNHES VQWCYDNFIN YRSLMSADNV RQQLSRIMDR FNLPRRSTDF TSRDYYINIR KALVTGYFMQ VAHLERTGHY LTVKDNQVVQ LHPSTVLDHK PEWVLYNEFV LTTKNYIRTC TDIKPEWLVK IAPQYYDMSN FPQCEAKRQL DRIIAKLQSK EYSQY
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Pre-mRNA processing factor involved in disassembly of spliceosomes after the release of mature mRNA. In cooperation with TFIP11 seem to be involved in the transition of the U2, U5 and U6 snRNP-containing IL complex to the snRNP-free IS complex leading to efficient debranching and turnover of excised introns.
  • 基因功能參考文獻:
    1. DHX15 regulates androgen receptor (AR) activity by modulating E3 ligase Siah2-mediated AR ubiquitination independent of its ATPase activity promoting prostate cancer progression. PMID: 28991234
    2. The crystal structure of human DHX15 reveals a domain organization of the mammalian DEAH/RHA family. PMID: 28580923
    3. Loss of DHX15 expression is associated with glioma. PMID: 28829764
    4. depletion of NKRF, XRN2 or DHX15 impairs an early pre-rRNA cleavage step. PMID: 28115624
    5. Mutation in DHX15 is associated with core-binding factor acute myeloid leukemia. PMID: 27798625
    6. Data indicate that DEAH-box RNA helicase DHX15/PRP43 stimulates the NF-kappaB and MAPK pathways downstream of virus-induced signaling adapter protein MAVS and contributes to MAVS-mediated cytokine production and apoptosis. PMID: 24782566
    7. The results suggest that activation of Prp43p by Gno1p/PINX1 within early pre-ribosomal particles is crucial for their subsequent maturation. PMID: 24823796
    8. Data suggest that G-patch domain of RBM5 is indispensable for its ability to interact with DHX15; RBM5 stimulates helicase activity of DHX15 in a G-patch domain-dependent manner. PMID: 22569250

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  • 亞細胞定位:
    Nucleus. Nucleus, nucleolus.
  • 蛋白家族:
    DEAD box helicase family, DEAH subfamily, DDX15/PRP43 sub-subfamily
  • 組織特異性:
    Ubiquitous.
  • 數據庫鏈接:

    HGNC: 2738

    OMIM: 603403

    KEGG: hsa:1665

    STRING: 9606.ENSP00000336741

    UniGene: Hs.696074



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