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Recombinant Human N-acylethanolamine-hydrolyzing acid amidase (NAAA ASAHL PLT)

  • 中文名稱:
  • 貨號:
    CSB-YP2291HU
  • 規格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
  • 貨號:
    CSB-EP2291HU
  • 規格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
  • 貨號:
    CSB-EP2291HU-B
  • 規格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
  • 貨號:
    CSB-BP2291HU
  • 規格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
  • 貨號:
    CSB-MP2291HU
  • 規格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    NAAA
  • Uniprot No.:
  • 別名:
    N-acylethanolamine-hydrolyzing acid amidase; EC 3.5.1.60; Acid ceramidase-like protein; Acylsphingosine deacylase NAAA; EC 3.5.1.23; N-acylsphingosine amidohydrolase-like; ASAH-like protein; [Cleaved into: N-acylethanolamine-hydrolyzing acid amidase subunit alpha; N-acylethanolamine-hydrolyzing acid amidase subunit beta] NAAA ASAHL PLT
  • 種屬:
    Homo sapiens (Human)
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Degrades bioactive fatty acid amides to their corresponding acids, with the following preference: N-palmitoylethanolamine > N-myristoylethanolamine > N-lauroylethanolamine = N-stearoylethanolamine > N-arachidonoylethanolamine > N-oleoylethanolamine. Also exhibits weak hydrolytic activity against the ceramides N-lauroylsphingosine and N-palmitoylsphingosine.
  • 基因功能參考文獻:
    1. Variants a1 and a2 encoded the same full-length NAAA protein. PMID: 27693242
    2. The results suggest that N-acylethanolamine acid amidase and protein tyrosine kinase 7 may be used as potential tissue biomarkers to avoid overtreatment of non-aggressive prostate cancer PMID: 24741114
    3. MALDI-TOF MS analysis of the human NAAA zymogen (47.7 kDa) treated with peptide-N-glycosidase F (PNGase F) identified 4 glycosylation sites, and acid cleavage of the zymogen into alpha- and beta-subunits (14.6 and 33.3 kDa) activated the enzyme. PMID: 22040171
    4. The level and activity of acid ceramidase in Alzheimer disease (AD) brain may play a role in controlling neuronal apoptosis and may mediate signalling pathways involved in the molecular mechanism of AD. PMID: 15610181
    5. Asn-37, Asn-107, Asn-309, and Asn-333 are actual N-glycosylation sites. The glycosylation appeared to play an important role in stabilizing the enzyme protein. PMID: 17980170
    6. we describe the overexpression and processing of recombinant human acid ceramidase in insect cells, its purification and characterization PMID: 18020949
    7. These results showed that both N-acylethanolamine-hydrolysing acid amidase and fatty acid amide hydrolase are functionally active in human prostate cancer cells. PMID: 18806270

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  • 亞細胞定位:
    Lysosome. Membrane; Peripheral membrane protein.
  • 蛋白家族:
    Acid ceramidase family
  • 組織特異性:
    Expressed in numerous tissues, with highest levels in liver and kidney, followed by pancreas.
  • 數據庫鏈接:

    HGNC: 736

    OMIM: 607469

    KEGG: hsa:27163

    STRING: 9606.ENSP00000286733

    UniGene: Hs.437365



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