Recombinant Human Kinesin-like protein KIFC1 (KIFC1)

中文名稱：

人KIFC1重組蛋白
貨號：

CSB-EP871587HU
規格：

￥1836
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
基因名：

KIFC1
Uniprot No.：

Q9BW19
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length
來源：

E.coli
分子量：

81.2 kDa
表達區域：

1-673aa
氨基酸序列

MDPQRSPLLEVKGNIELKRPLIKAPSQLPLSGSRLKRRPDQMEDGLEPEKKRTRGLGATTKITTSHPRVPSLTTVPQTQGQTTAQKVSKKTGPRCSTAIATGLKNQKPVPAVPVQKSGTSGVPPMAGGKKPSKRPAWDLKGQLCDLNAELKRCRERTQTLDQENQQLQDQLRDAQQQVKALGTERTTLEGHLAKVQAQAEQGQQELKNLRACVLELEERLSTQEGLVQELQKKQVELQEERRGLMSQLEEKERRLQTSEAALSSSQAEVASLRQETVAQAALLTEREERLHGLEMERRRLHNQLQELKGNIRVFCRVRPVLPGEPTPPPGLLLFPSGPGGPSDPPTRLSLSRSDERRGTLSGAPAPPTRHDFSFDRVFPPGSGQDEVFEEIAMLVQSALDGYPVCIFAYGQTGSGKTFTMEGGPGGDPQLEGLIPRALRHLFSVAQELSGQGWTYSFVASYVEIYNETVRDLLATGTRKGQGGECEIRRAGPGSEELTVTNARYVPVSCEKEVDALLHLARQNRAVARTAQNERSSRSHSVFQLQISGEHSSRGLQCGAPLSLVDLAGSERLDPGLALGPGERERLRETQAINSSLSTLGLVIMALSNKESHVPYRNSKLTYLLQNSLGGSAKMLMFVNISPLEENVSESLNSLRFASKVNQCVIGTAQANRK
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 10xHis-tagged and C-terminal Myc-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Minus end-directed microtubule-dependent motor required for bipolar spindle formation. May contribute to movement of early endocytic vesicles. Regulates cilium formation and structure.
基因功能參考文獻：
1. Data show that kinesin-related protein HSET (HSET) is incapable of forming asters from preformed, nongrowing microtubule (non-MT) MTs, but rapidly forms MT asters in the presence of soluble (non-MT) tubulin. PMID: 29985404
2. KIFC1 interacts with F508del-CFTR. PMID: 30066085
3. nuclear KIFC1 (nKIFC1) correlated significantly with Ki67 in White triple-negative breast cancer (TNBCs) but not in African American (AA) TNBCs, suggesting that nKIFC1 is not merely a surrogate for proliferation in AA TNBCs. PMID: 28218233
4. Results identified Stathmin as a direct target of KIFC1. PMID: 28885720
5. The overexpression of KIFC1 was correlated closely with the progression of hepatocellular carcinoma (HCC) and poor prognosis, and suggested that the expression levels of KIFC1 are a potential prognostic biomarker for HCC. PMID: 29620256
6. Circulating pri-miRNA-944 and 3662 can improve non-invasive potential non-small cell lung cancer detection of operable stages of SCC and AC PMID: 28964573
7. These findings reveal a new stationary binding model that kinesin-14 KIFC1 proteins function as crosslinkers between the Golgi apparatus and the microtubules and contribute to the central positioning and structural maintenance of the Golgi apparatus. PMID: 28430595
8. Taken together, these results suggest that up-regulation of KIFC1 may induce docetaxel resistance. PMID: 27665358
9. Low post translational modifications of KIFC1 protein is associated with neoplasms. PMID: 28209915
10. KIFC1 is overexpressed in GC. PMID: 27176706
11. Association of KIFC1 gene expression and centrosome amplification with high-grade serous ovarian carcinoma. PMID: 26992853
12. stabilization of the centrosome-spindle pole interface by the CEP215-HSET complex could promote survival of cancer cells containing supernumerary centrosomes. PMID: 26987684
13. KIFC1 silencing significantly reduces breast cancer cell viability. PMID: 26177331
14. Data provides the first evidence of centrosome clustering-independent activities of HSET that fuel tumor progression. PMID: 25788277
15. We show that some are new substrates of the anaphase-promoting complex/cyclosome and validate KIFC1 and RacGAP1/Cyk4 as two such targets involved respectively in timely mitotic spindle disassembly and cell spreading PMID: 24857844
16. Our data underscore KIFC1 as a putative biomarker that predicts worse prognosis, poor overall survival and may serve as a potential marker of onset of metastatic dissemination in ovarian cancer patients. PMID: 25028599
17. Study demonstrates microtubule tip-tracking of human kinesin-14 HSET and show that EB1 is sufficient to induce this dynamic microtubule end localization. PMID: 24039245
18. Acentrosomal spindle assembly mechanisms are hyperactive in cancer cells and promote HSET. PMID: 22946058
19. we suggest that KIFC1 plays an essential role for bipolar MTOC formation and maintaining chromosomal stability in the mitosis . PMID: 23318213
20. A case-control analysis was carried out by comparing the genotype distribution of six KIFC1 single-nucleotide polymorphisms between 93 aspirin exacerbated respiratory disease cases and 96 aspirin-tolerant asthma controls in a Korean population PMID: 22201025
21. The intense placental expression of KIFC1 in syncytiotrophoblast and KIF17 in vascular endothelium suggests that both proteins might be important in a cargo-transport system. KIFC1 and KIF17 expression are increased of both in preeclamptia and diabetes. PMID: 19679349
22. An analysis was made of the weak interactions of ADP-Unc104 and ADP-kinesin with microtubules and their inhibition by MAP2. PMID: 17326138
23. HSET has a role in controlling spindle length by cross-linking and sliding microtubules. PMID: 19116309
24. High KIFC1 expression is associated with metastatic spread to the brain in primary non-small cell lung cancer. PMID: 19190132
25. Chromosome congression in HSET + hNuf2 co-depleted cells required the plus-end directed motor CENP-E , which has been implicated in the gliding of mono-oriented kinetochores alongside adjacent K-fibres. PMID: 19525938
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亞細胞定位：

Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Early endosome.
蛋白家族：

TRAFAC class myosin-kinesin ATPase superfamily, Kinesin family, NCD subfamily
數據庫鏈接：

HGNC: 6389

OMIM: 603763

KEGG: hsa:3833

STRING: 9606.ENSP00000393963

UniGene: Hs.436912