Recombinant Human GTPase ERas (ERAS), partial

中文名稱：

人ERAS重組蛋白
貨號：

CSB-EP801806HU1
規格：

￥1344
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
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靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

ERAS
Uniprot No.：

Q7Z444
別名：

E ras; E-Ras; ecat5; Embryonic stem cell expressed Ras ; Embryonic stem cell-expressed Ras; Eras; ES cell expressed Ras; GTPase ERas; Ha-Ras2; HRAS2; HRASP; MGC126691; MGC126693; OTTHUMP00000065857 ; RASE_HUMAN; Small GTPase protein E Ras; v Ha ras Harvey rat sarcoma viral oncogene homolog pseudogene ; v Ha ras Harvey rat sarcoma viral oncogene homolog 2
種屬：

Homo sapiens (Human)
蛋白長度：

Partial
來源：

E.coli
分子量：

28.8kDa
表達區域：

3-230aa
氨基酸序列

LPTKPGTFDLGLATWSPSFQGETHRAQARRRDVGRQLPEYKAVVVGASGVGKSALTIQLNHQCFVEDHDPTIQDSYWKELTLDSGDCILNVLDTAGQAIHRALRDQCLAVCDGVLGVFALDDPSSLIQLQQIWATWGPHPAQPLVLVGNKCDLVTTAGDAHAAAAALAHSWGAHFVETSAKTRQGVEEAFSLLVHEIQRVQEAMAKEPMARSCREKTRHQKATCHCGC
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
產品描述：

The production of this Recombinant Human ERAS protein started with the ERAS gene synthesis. And then using recombinant DNA technology, the ERAS gene was inserted into an expression vector so that we could get the recombinant express plasmid of ERAS. Transform the plasmid into the cells of E.coli, culture the cells and we could get the desired Recombinant Human ERAS protein. But the work was not completed, protein purification and a strict QC system were performed in the last step. The purity is 90%+ determined by SDS-PAGE.

ERAS (also named HRAS2 or HRASP) is a gene providing an instruction of making a protein named GTPase ERas (ERas) in human. The protein encoded by this gene is also known as Embryonic stem cell-expressed Ras and belongs to Ras family found in murine embryonic stem (ES) cells in 2003. Increasing studies has been reported that ERAS may provide resistance to chemotherapy in different human tumorigenic cells through activation of the PI3K/AKT/mTOR pathway, promoting both DNA replication and transcription.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the tumor-like growth properties of embryonic stem cells.
基因功能參考文獻：
1. N-terminal extension of E-RAS is important for E-RAS signaling activity. PMID: 25940089
2. Expression of ERAS in human melanoma cell lines conferred resistance to PLX4720 and induced hyperphosphorylation of AKT. PMID: 25624498
3. After knocking down the ERas gene by siRNA, we observed that there was a significant decrease in proliferation, metastasis as well as clonality in gastric carcinoma. PMID: 23612786
4. Data indicate that expression of ERAS, LHX1, and CCRK is increased in aggressive subgroups of medulloblastomas. PMID: 22875024
5. ERas may be a potential biomarker for gastric cancer, but advanced studies are wanted. PMID: 21151392
6. These data suggest that ERas is activated in a significant population of gastric cancer, where it may play a crucial role in gastric cancer cell survival and metastases to liver via down-regulation of E-cadherin. PMID: 20566745
7. ERas/PI3K pathway may provide resistance to chemotherapy and promote transforming activity in neuroblastoma. PMID: 20811723
8. transforming oncogene ERas is important in the tumour-like growth properties of embryonic stem cells PMID: 12774123
9. Studies on ERas gene expression. PMID: 16081664
10. rasH2 may have a role in susceptibility to chemically-induced tongue and esophageal neoplasms PMID: 18174262
11. High ERas is associated with the tumorigenic process of gastric carcinomas. PMID: 19528480
12. Loss of methylation in the promoter of ERas might be one of mechanisms responsible for the re-expression of an embryonic oncogene in gastric cancer. PMID: 19787253
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亞細胞定位：

Cell membrane; Lipid-anchor; Cytoplasmic side.
蛋白家族：

Small GTPase superfamily, Ras family
數據庫鏈接：

HGNC: 5174

OMIM: 300437

KEGG: hsa:3266

STRING: 9606.ENSP00000339136

UniGene: Hs.447330