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  4. Recombinant Human DNA-directed RNA polymerase I subunit RPA2 (POLR1B), partial

Recombinant Human DNA-directed RNA polymerase I subunit RPA2 (POLR1B), partial

  • 中文名稱：
    人POLR1B重組蛋白
  • 貨號：
    CSB-YP872528HU
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    人POLR1B重組蛋白
  • 貨號：
    CSB-EP872528HU
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    人POLR1B重組蛋白
  • 貨號：
    CSB-EP872528HU-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    人POLR1B重組蛋白
  • 貨號：
    CSB-BP872528HU
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    人POLR1B重組蛋白
  • 貨號：
    CSB-MP872528HU
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    POLR1B
  • Uniprot No.：
  • 別名：
    A127; DNA directed RNA polymerase I 135kDa polypeptide; DNA directed RNA polymerase I; DNA directed RNA polymerase I subunit RPA2; DNA-directed RNA polymerase I 135 kDa polypeptide; DNA-directed RNA polymerase I subunit rpa2; FLJ10816; FLJ21921; MGC131780; OTTHUMP00000161989; OTTHUMP00000203643; OTTHUMP00000203645; POLR 1B; polr1b; Polymerase (RNA) I polypeptide B 128kDa; Polymerase (RNA) I polypeptide B; RNA polymerase I polypeptide B; RNA polymerase I subunit 2; RPA 135; RPA116; RPA135; RPA2; RPA2_HUMAN; Rpo1 2
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol I is composed of mobile elements and RPA2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft.
  • 基因功能參考文獻：
    1. PTEN represses RNA Pol I transcription through a novel mechanism that involves disruption of the SL1 complex PMID: 16055704
    2. CK2 has the potential to regulate Pol I transcription at multiple levels, in preinitiation complex (PIC) formation, activation, and reinitiation of transcription. PMID: 16880508
    3. Human Maf1 protein negatively regulates transcription by all three nuclear Pols. Changes in Maf1 expression affect Pol I-dependent transcription in human glioblastoma lines. PMID: 17499043
    4. The average FIX expression level of the rDNA recombinants was additionally enhanced to that from a strong Pol II promoter as a result of elimination of position effects. PMID: 18194663
  • 亞細胞定位：
    Nucleus, nucleolus. Chromosome.
  • 蛋白家族：
    RNA polymerase beta chain family
  • 數據庫鏈接：

    HGNC: 20454

    OMIM: 602000

    KEGG: hsa:84172

    STRING: 9606.ENSP00000263331

    UniGene: Hs.745127



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