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Recombinant Human Crossover junction endonuclease EME1 (EME1)

  • 中文名稱:
    人EME1重組蛋白
  • 貨號:
    CSB-YP856897HU
  • 規格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    人EME1重組蛋白
  • 貨號:
    CSB-EP856897HU-B
  • 規格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    人EME1重組蛋白
  • 貨號:
    CSB-BP856897HU
  • 規格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    人EME1重組蛋白
  • 貨號:
    CSB-MP856897HU
  • 規格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    EME1
  • Uniprot No.:
  • 別名:
    6820428D13; Crossover junction endonuclease EME1; EC 3.1.22.-; EME1; Eme1 essential meiotic endonuclease 1 homolog 1 (S. pombe); EME1; S. pombe; homolog of; 1; EME1_HUMAN; essential meiotic endonuclease 1 homolog 1 (S pombe); Essential Meiotic Endonuclease 1 Homolog 1; essential meiotic endonuclease 1 homolog 2; Essential meiotic endonuclease 1; S. pombe; homolog of; 1; FLJ31364; hMMS4; homolog of yeast EME1 endonuclease; MGC106543; MMS4 ; MMS4 homolog; MMS4L
  • 種屬:
    Homo sapiens (Human)
  • 蛋白長度:
    full length protein
  • 表達區域:
    1-570
  • 氨基酸序列
    MALKKSSPSL DSGDSDSEEL PTFAFLKKEP SSTKRRQPER EEKIVVVDIS DCEASCPPAP ELFSPPVPEI AETVTQTQPV RLLSSESEDE EEFIPLAQRL TCKFLTHKQL SPEDSSSPVK SVLDHQNNEG ASCDWKKPFP KIPEVPLHDT PERSAADNKD LILDPCCQLP AYLSTCPGQS SSLAVTKTNS DILPPQKKTK PSQKVQGRGS HGCRQQRQAR QKESTLRRQE RKNAALVTRM KAQRPEECLK HIIVVLDPVL LQMEGGGQLL GALQTMECRC VIEAQAVPCS VTWRRRAGPS EDREDWVEEP TVLVLLRAEA FVSMIDNGKQ GSLDSTMKGK ETLQGFVTDI TAKTAGKALS LVIVDQEKCF SAQNPPRRGK QGANKQTKKQ QQRQPEASIG SMVSRVDAEE ALVDLQLHTE AQAQIVQSWK ELADFTCAFT KAVAEAPFKK LRDETTFSFC LESDWAGGVK VDLAGRGLAL VWRRQIQQLN RVSLEMASAV VNAYPSPQLL VQAYQQCFSD KERQNLLADI QVRRGEGVTS TSRRIGPELS RRIYLQMTTL QPHLSLDSAD
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.
  • 基因功能參考文獻:
    1. The data suggest that subnuclear relocalization is relevant for the function of Mus81-Mms4 complex in response to DNA damage and, probably, of the endonucleases that colocalize with it. PMID: 28813668
    2. EME-1 deficiency affects cell cycle progression and promotes DNA rereplication. PMID: 16456034
    3. Low Eme1 levels were more sensitive to the drug than tumors with high levels. PMID: 19267403
  • 亞細胞定位:
    Nucleus, nucleolus. Note=Recruited to regions of DNA damage in S-phase cells.
  • 蛋白家族:
    EME1/MMS4 family
  • 數據庫鏈接:

    HGNC: 24965

    OMIM: 610885

    KEGG: hsa:146956

    STRING: 9606.ENSP00000376952

    UniGene: Hs.514330



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