Recombinant Human 7SK snRNA methylphosphate capping enzyme (MEPCE)

中文名稱：

人MEPCE重組蛋白
貨號：

CSB-YP762569HU
規(guī)格：
來源：

Yeast
其他：

中文名稱：

人MEPCE重組蛋白
貨號：

CSB-EP762569HU
規(guī)格：
來源：

E.coli
其他：

中文名稱：

人MEPCE重組蛋白
貨號：

CSB-EP762569HU-B
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

人MEPCE重組蛋白
貨號：

CSB-BP762569HU
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

人MEPCE重組蛋白
貨號：

CSB-MP762569HU
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

MEPCE
Uniprot No.：

Q7L2J0
別名：

MEPCE; BCDIN37SK snRNA methylphosphate capping enzyme; MePCE; EC 2.1.1.-; Bicoid-interacting protein 3 homolog; Bin3 homolog
種屬：

Homo sapiens (Human)
蛋白長度：

full length protein
表達(dá)區(qū)域：

1-689
氨基酸序列

MIEMAAEKEP FLVPAPPPPL KDESGGGGGP TVPPHQEAAS GELRGGTERG PGRCAPSAGS PAAAVGRESP GAAATSSSGP QAQQHRGGGP QAQSHGEARL SDPPGRAAPP DVGEERRGGG GTELGPPAPP RPRNGYQPHR PPGGGGGKRR NSCNVGGGGG GFKHPAFKRR RRVNSDCDSV LPSNFLLGGN IFDPLNLNSL LDEEVSRTLN AETPKSSPLP AKGRDPVEIL IPKDITDPLS LNTCTDEGHV VLASPLKTGR KRHRHRGQHH QQQQAAGGSE SHPVPPTAPL TPLLHGEGAS QQPRHRGQNR DAPQPYELNT AINCRDEVVS PLPSALQGPS GSLSAPPAAS VISAPPSSSS RHRKRRRTSS KSEAGARGGG QGSKEKGRGS WGGRHHHHHP LPAAGFKKQQ RKFQYGNYCK YYGYRNPSCE DGRLRVLKPE WFRGRDVLDL GCNVGHLTLS IACKWGPSRM VGLDIDSRLI HSARQNIRHY LSEELRLPPQ TLEGDPGAEG EEGTTTVRKR SCFPASLTAS RGPIAAPQVP LDGADTSVFP NNVVFVTGNY VLDRDDLVEA QTPEYDVVLC LSLTKWVHLN WGDEGLKRMF RRIYRHLRPG GILVLEPQPW SSYGKRKTLT ETIYKNYYRI QLKPEQFSSY LTSPDVGFSS YELVATPHNT SKGFQRPVYL FHKARSPSH
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評價

靶點(diǎn)詳情

功能：

S-adenosyl-L-methionine-dependent methyltransferase that adds a methylphosphate cap at the 5'-end of 7SK snRNA (7SK RNA), leading to stabilize it. Also has a non-enzymatic function as part of the 7SK RNP complex: the 7SK RNP complex sequesters the positive transcription elongation factor b (P-TEFb) in a large inactive 7SK RNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. The 7SK RNP complex also promotes snRNA gene transcription by RNA polymerase II via interaction with the little elongation complex (LEC). In the 7SK RNP complex, MEPCE is required to stabilize 7SK RNA and facilitate the assembly of 7SK RNP complex. MEPCE has a non-enzymatic function in the 7SK RNP complex; interaction with LARP7 within the 7SK RNP complex occluding its catalytic center.
基因功能參考文獻(xiàn)：
1. The 7SK small nuclear RNP (snRNP), composed of the 7SK small nuclear RNA (snRNA), MePCE, and Larp7, also functions as a canonical transcription factor that, in collaboration with the little elongation complex (LEC) comprising ELL, Ice1, Ice2, and ZC3H8, promotes transcription of RNAPII-specific spliceosomal snRNA and small nucleolar RNA (snoRNA) genes. PMID: 28254838
2. Data indicate the binding of RNA-binding protein HEXIM with 7SKsnRNP complex comprising the non-coding RNA 7SK and proteins MePCE and LARP7. PMID: 25863285
3. Capping enzyme can play a direct role in transcription by modulating displacement of nascent RNA during transcription. PMID: 17978174
亞細(xì)胞定位：

Nucleus.
蛋白家族：

Methyltransferase superfamily
組織特異性：

Expressed in chronic myeloid leukemia cells, adrenal gland, brain, cerebellum, kidney, lung, mammary gland and testis. Weakly or not expressed in other tissues.
數(shù)據(jù)庫鏈接：

HGNC: 20247

OMIM: 611478

KEGG: hsa:56257

STRING: 9606.ENSP00000308546

UniGene: Hs.178011