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  4. Recombinant Escherichia coli Ribosomal RNA small subunit methyltransferase A (rsmA)

Recombinant Escherichia coli Ribosomal RNA small subunit methyltransferase A (rsmA)

  • 中文名稱：
    大腸桿菌rsmA重組蛋白
  • 貨號：
    CSB-YP356993ENV
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    大腸桿菌rsmA重組蛋白
  • 貨號：
    CSB-EP356993ENV
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    大腸桿菌rsmA重組蛋白
  • 貨號：
    CSB-EP356993ENV-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    大腸桿菌rsmA重組蛋白
  • 貨號：
    CSB-BP356993ENV
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    大腸桿菌rsmA重組蛋白
  • 貨號：
    CSB-MP356993ENV
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    rsmA
  • Uniprot No.：
  • 別名：
    rsmA; ksgA; b0051; JW0050; Ribosomal RNA small subunit methyltransferase A; EC 2.1.1.182; 16S rRNA; adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; High level kasugamycin resistance protein KsgA; Kasugamycin dimethyltransferase; S-adenosylmethionine-6-N'; N'-adenosyl(rRNA) dimethyltransferase
  • 種屬：
    Escherichia coli (strain K12)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區域：
    2-273
  • 氨基酸序列
    NNRVHQGHL ARKRFGQNFL NDQFVIDSIV SAINPQKGQA MVEIGPGLAA LTEPVGERLD QLTVIELDRD LAARLQTHPF LGPKLTIYQQ DAMTFNFGEL AEKMGQPLRV FGNLPYNIST PLMFHLFSYT DAIADMHFML QKEVVNRLVA GPNSKAYGRL SVMAQYYCNV IPVLEVPPSA FTPPPKVDSA VVRLVPHATM PHPVKDVRVL SRITTEAFNQ RRKTIRNSLG NLFSVEVLTG MGIDPAMRAE NISVAQYCQM ANYLAENAPL QES
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits. Has also a DNA glycosylase/AP lyase activity that removes C mispaired with oxidized T from DNA, and may play a role in protection of DNA against oxidative stress.
  • 基因功能參考文獻：
    1. Overexpression of RbfA in the absence of the KsgA results in impaired translation initiation. PMID: 23387871
    2. A key aspect of KsgA's mechanism for regulating ribosome biogenesis is the ability of the enzyme to dimethylate its two target adenosines. PMID: 22142337
    3. Helix 24 and helix 45 of KsgA play important roles in catalysis of 16S rRNA. PMID: 21142019
    4. This study suggests that KsgA has another unidentified function that plays a role in the suppression of the cold-sensitive phenotype of the Era(E200K) strain and that this function may be involved in the acid shock response. PMID: 17890303
    5. KsgA functions as a late-stage ribosome biogenesis factor, and methylation is a trigger for release of KsgA from the assembling subunits. Gene deletion leads to cold sensitivity and altered ribosomal profiles. PMID: 18990185

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  • 亞細胞定位：
    Cytoplasm.
  • 蛋白家族：
    Class I-like SAM-binding methyltransferase superfamily, rRNA adenine N(6)-methyltransferase family, RsmA subfamily
  • 數據庫鏈接：


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