在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

Recombinant Escherichia coli 8-oxo-dGTP diphosphatase (mutT)

  • 中文名稱:
    Recombinant Escherichia coli 8-oxo-dGTP diphosphatase(mutT)
  • 貨號:
    CSB-BP357355ENV
  • 規格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    Recombinant Escherichia coli 8-oxo-dGTP diphosphatase(mutT)
  • 貨號:
    CSB-EP357355ENV-B
  • 規格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    Recombinant Escherichia coli 8-oxo-dGTP diphosphatase(mutT)
  • 貨號:
    CSB-MP357355ENV
  • 規格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    Name:mutT Ordered Locus Names:b0099, JW0097
  • Uniprot No.:
  • 別名:
    mutT; b0099; JW0097; 8-oxo-dGTP diphosphatase; 8-oxo-dGTPase; EC 3.6.1.55; 7,8-dihydro-8-oxoguanine-triphosphatase; Mutator protein MutT; dGTP pyrophosphohydrolase
  • 種屬:
    Escherichia coli (strain K12)
  • 蛋白長度:
    full length protein
  • 表達區域:
    1-129
  • 氨基酸序列
    MKKLQIAVGI IRNENNEIFI TRRAADAHMA NKLEFPGGKI EMGETPEQAV VRELQEEVGI TPQHFSLFEK LEYEFPDRHI TLWFWLVERW EGEPWGKEGQ PGEWMSLVGL NADDFPPANE PVIAKLKRL
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Specifically hydrolyzes both 8-oxo-deoxyguanosine triphosphate (8-oxo-dGTP) and 8-oxo-guanosine triphosphate (8-oxo-GTP) to the related monophosphates, thereby cleaning up the nucleotide pools and preventing misincorporation of 8-oxoGua into DNA and RNA. It prevents replicational errors by removing an oxidatively damaged form of guanine (8-oxo-dGTP) from DNA and the nucleotide pool. 8-oxo-dGTP can be inserted opposite dA and dC residues of template DNA with almost equal efficiency thus leading to A.T to G.C transversions. MutT may also ensure transcriptional fidelity, removing 8-oxo-GTP from the ribonucleotide triphosphate pool. However, due to the lower efficiency of RNA polymerase 8-oxo-GTP incorporation, MutT is probably not a major contributor to transcriptional fidelity. It also hydrolyzes 8-oxo-dGDP and 8-oxo-GDP to their monophosphate form. In vitro, can also use dGTP, dGDP and other various nucleoside di- and triphosphates, with much lower efficiency. Works cooperatively with MutM and MutY to prevent accumulation in the DNA of oxidized guanine residues.
  • 基因功能參考文獻:
    1. The study investigated the role of MutT on transcription fidelity and find no increase in epigenetic switch frequency in the absence of MutT function. PMID: 25294823
    2. MutT mutation increased the point-mutation rate 150-fold. PMID: 23248287
    3. The MutT functions are not needed under anaerobic condition, yet the level of the MutT protein in cell is kept constant, probably for preparing for sudden changes of oxygen pressure. PMID: 21147134
    4. MutT hydrolyzes four types of 8-oxoGua (8-oxo-7,8-dihydroguanine)-containing nucleoside diphosphates and triphosphates to the related monophosphates, thereby preventing misincorporation of 8-oxoGua into DNA and RNA. PMID: 15850400
    5. transient state kinetic analysis of the reaction pathway of mutT; findings confirm an iso kinetic mechanism and explain the preferred hydrolysis of 8-oxo-dGTP PMID: 16285737
    6. These results indicate that mutT plays important roles in mutagenesis induced by 2-OH-dATP and 8-OH-dGTP in vivo. PMID: 17150583
    7. The mutT defect does not elevate chromosomal fragmentation in Escherichia coli because of the surprisingly low levels of MutM/MutY-recognized DNA modifications. PMID: 17616589

    顯示更多

    收起更多

  • 蛋白家族:
    Nudix hydrolase family
  • 數據庫鏈接:


主站蜘蛛池模板: 欧美丰满熟妇xxxx| 亚洲人成色77777| 亚洲精品乱码久久久久久蜜桃图片| 国产suv精品一区二区6| 波多野结衣爽到高潮大喷| 中本亚洲欧美国产日韩| 国产成人精品日本亚洲网站| 国产成人无码综合亚洲日韩| 日韩精品人妻无码久久影院| 亚洲国产区男人本色vr| 久久天天躁狠狠躁夜夜2020一| 国产人成视频在线视频| 成人精品动漫一区二区| 亚洲精品美女久久777777| 欧美丰满少妇xxxxx| 日韩久久无码免费毛片软件 | 97久久婷婷五月综合色d啪蜜芽| 国产乱子伦在线一区二区| 青草草在线视频永久免费| 国产精品99久久不卡| 国产成人亚洲综合网站小说| 97久久草草超级碰碰碰| 日本乱人伦在线观看| 老子午夜理论影院理论| 又色又爽又黄还免费毛片96下载| 国内精品视频一区二区三区| 亚洲一区二区三区av无码| 亚洲精品av一二三区无码 | 野外做受三级视频| 51久久成人国产精品麻豆| 久久视频在线视频精品| 伦埋琪琪久久影院三级| 亚洲熟妇av乱码在线观看| 一区二区三区四区在线 | 中国 | 国产青榴视频在线观看| 国产成人无码免费看片软件| 国产成人无码a区在线| 免费看小12萝裸体视频国产| 成熟人妻av无码专区| 在厨房拨开内裤进入毛片| 性色av无码中文av有码vr|