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  4. Recombinant Enterobacteria phage MX1 RNA-directed RNA polymerase beta chain, partial

Recombinant Enterobacteria phage MX1 RNA-directed RNA polymerase beta chain, partial

  • 中文名稱：
    Recombinant Enterobacteria phage MX1 RNA-directed RNA polymerase beta chain ,partial
  • 貨號：
    CSB-YP526479ELC
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Enterobacteria phage MX1 RNA-directed RNA polymerase beta chain ,partial
  • 貨號：
    CSB-EP526479ELC
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Enterobacteria phage MX1 RNA-directed RNA polymerase beta chain ,partial
  • 貨號：
    CSB-EP526479ELC-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Enterobacteria phage MX1 RNA-directed RNA polymerase beta chain ,partial
  • 貨號：
    CSB-BP526479ELC
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Enterobacteria phage MX1 RNA-directed RNA polymerase beta chain ,partial
  • 貨號：
    CSB-MP526479ELC
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    N/A
  • Uniprot No.：
  • 別名：
    RNA-directed RNA polymerase subunit beta; EC 2.7.7.48; RNA replicase beta chain
  • 種屬：
    Enterobacteria phage MX1 (Bacteriophage MX1)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    This is the catalytic subunit of the viral RNA-dependent RNA polymerase complex. This complex is involved in viral RNA replication that produces (+)-stranded genomes via a complementary, (-)-stranded intermediate. Binds RNA cooperatively with the host ribosomal protein S1.
  • 基因功能參考文獻：
    1. The data suggest that the beta-subunit and protein S1 cooperatively bind the (+)-stranded Qbeta genome during replication initiation. PMID: 26578560
    2. The third mobile OB-fold of ribosomal protein S1, which is spatially anchored near the surface of the Qbeta replicase beta-subunit, primarily recruits the Qbeta RNA toward the beta-subunit, leading to the specific and efficient replication initiation of Qbeta RNA. PMID: 25122749
    3. Thr210Ala and Tyr410His mutation in replicase had little or no effect on replication fidelity. PMID: 24965463
    4. Data indicate that ribosomes enhanced maximum rate of RNA synthesis by Qbeta replicase. PMID: 24269228
    5. EF-Tu in Qbeta replicase modulates RNA elongation processes in a distinct manner from its established function in protein synthesis. PMID: 22245970
    6. Cys533 of the beta Subunit is responsible for replicase Stability. PMID: 20858892
    7. crystal structure of the complex between the beta-subunit and the two host proteins EF-Tu and EF-Ts to 2.5-A resolution PMID: 20534494

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  • 數據庫鏈接：

    KEGG: vg:1261500



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