Recombinant Drosophila melanogaster Sphingosine-1-phosphate lyase (Sply), partial
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中文名稱:黑腹果蠅Sply重組蛋白
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貨號:CSB-EP895577DLU-B
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說明書:
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規(guī)格:
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來源:E.coli
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共軛:Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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其他:
產(chǎn)品詳情
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純度:>85% (SDS-PAGE)
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基因名:Sply
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Uniprot No.:
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別名:Sply; Spl; CG8946; Sphingosine-1-phosphate lyase; S1PL; SP-lyase; SPL; EC 4.1.2.27; Sphingosine-1-phosphate aldolase
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種屬:Drosophila melanogaster (Fruit fly)
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蛋白長度:Partial
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蛋白標簽:Tag?type?will?be?determined?during?the?manufacturing?process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially. -
產(chǎn)品提供形式:Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. -
復溶:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
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儲存條件:Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
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保質(zhì)期:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. -
貨期:Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
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注意事項:Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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Datasheet :Please contact us to get it.
靶點詳情
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功能:Cleaves phosphorylated sphingoid bases (PSBs), such as sphingosine-1-phosphate, into fatty aldehydes and phosphoethanolamine. Sphingolipid catabolism is required for normal development including viability, reproduction and muscle development.
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基因功能參考文獻:
- In Drosophila, Sply mutants, which lack SGPL1, displayed a phenotype reminiscent of nephrotic syndrome in nephrocytes. PMID: 28165339
- cloning of the sphingosine-1-phosphate lyase gene (Sply) and its importance for adult muscle development and integrity, reproduction and larval viability PMID: 12702658
- There are defects in reproductive structures and function that are associated with enhanced apoptosis in Drosophila Sply05091 mutants that lack functional S1P lyase and thereby accumulate sphingolipid long chain base metabolites. PMID: 17706961
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亞細胞定位:Endoplasmic reticulum membrane; Single-pass type III membrane protein.
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蛋白家族:Group II decarboxylase family, Sphingosine-1-phosphate lyase subfamily
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組織特異性:Localized to the developing gut primordium during embryogenesis.
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