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  4. Recombinant Drosophila melanogaster Segmentation protein even-skipped (eve)

Recombinant Drosophila melanogaster Segmentation protein even-skipped (eve)

  • 中文名稱：
    黑腹果蠅eve重組蛋白
  • 貨號：
    CSB-YP361888DLU
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅eve重組蛋白
  • 貨號：
    CSB-EP361888DLU
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅eve重組蛋白
  • 貨號：
    CSB-EP361888DLU-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅eve重組蛋白
  • 貨號：
    CSB-BP361888DLU
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅eve重組蛋白
  • 貨號：
    CSB-MP361888DLU
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    eve
  • Uniprot No.：
  • 別名：
    eve; EVE_DROME; Segmentation protein even-skipped
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-376
  • 氨基酸序列
    MHGYRTYNME SHHAHHDASP VDQKPLVVDL LATQYGKPQT PPPSPNECLS SPDNSLNGSR GSEIPADPSV RRYRTAFTRD QLGRLEKEFY KENYVSRPRR CELAAQLNLP ESTIKVWFQN RRMKDKRQRI AVAWPYAAVY SDPAFAASIL QAAANSVGMP YPPYAPAAAA AAAAAAAVAT NPMMATGMPP MGMPQMPTMQ MPGHSGHAGH PSPYGQYRYT PYHIPARPAP PHPAGPHMHH PHMMGSSATG SSYSAGAAGL LGALPSATCY TGLGVGVPKT QTPPLDLQSS SSPHSSTLSL SPVGSDHAKV FDRSPVAQSA PSVPAPAPLT TTSPLPAPGL LMPSAKRPAS DMSPPPTTTV IAEPKPKLFK PYKTEA
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    May play a role in determining neuronal identity. May be directly involved in specifying identity of individual neurons. Pair-rule protein required for segmentation; involved in transforming the broad, spatial, aperiodic expression patterns of the gap genes into a system of precise periodic expression patterns of the pair-rule and segmentary polarity genes.
  • 基因功能參考文獻：
    1. Pericardial cells that express the transcription factor Even Skipped adopt distinct fates along the anterior-posterior axis. Among them, the most anterior Antp-Ubx-AbdA-negative cells form a novel cardiac outflow component we call the outflow hanging structure, whereas the Antp-expressing cells differentiate into wing heart precursors. PMID: 29247145
    2. natural variation in a conserved trait-even-skipped (eve) expression at the cellular blastoderm stage of embryonic development in Drosophila melanogaster, was investigated. PMID: 26129990
    3. Ubiquitous TER94 expression is "replaced" by expression in an eve pattern when Homie is deleted, and this effect is reversed when the PRE is also removed PMID: 24204298
    4. Deletion of any individual enhancer reduced Yan occupancy at the other elements, impacting eve expression, cell fate specification, and cardiac function PMID: 24186975
    5. Data strongly suggests that the eve-grn transcriptional code controls axon guidance, in part, by regulating the level of unc-5 expression. PMID: 22461564
    6. even-skipped genes were misexpressed in pho mutant embryos PMID: 22080372
    7. Nmo regulates a subset of Eve activities by stimulating Eve-mediated suppression of the odd-skipped (odd) repressor PMID: 20398650
    8. The repressor activity of Even-skipped is highly conserved, and is sufficient to activate engrailed and to regulate both the spacing and stability of parasegment boundaries. PMID: 12223400
    9. three distinct mechanisms are required for anterior repression of a single eve enhancer, each in a specific position PMID: 12397102
    10. the eve enhancer integrates multiple positive and negative transcription factor inputs to restrict eve expression to a single precursor cell and its motoneuron progeny PMID: 14556285
    11. opposing gradients of two Drosophila transcriptional repressors, Hunchback (Hb) and Knirps (Kni), position several segments by differentially repressing two distinct regulatory regions (enhancers) of the pair-rule gene even-skipped (eve) PMID: 14685241
    12. The pair-rule segmentation gene even skipped (eve) is required to activate engrailed stripes and to organize odd-numbered parasegments (PSs). PMID: 15110723
    13. Functional genetic complementation analysis of a eukaryotic cis-regulatory module-the even-skipped stripe 2 enhancer-from four Drosophila species. PMID: 15757364
    14. A high-throughput method allows for the quantification of eve expression in early embryos. PMID: 15834586
    15. The homeobox transcription factor even-skipped regulates netrin-receptor expression to control dorsal motor-axon projections in Drosophila. PMID: 16085495
    16. in developing Drosophila cardiac progenitor cells, the Eve precursors next to the Hh stripe are distinguished from more distant Lbe precursors by locally augmenting Ras signaling via elevating rho transcripts PMID: 16387294
    17. An eve-->grn-->zfh1 genetic cascade is unique to one of the intersegmental motoneurons, the aCC. PMID: 16540509
    18. A model of the transcriptional readout of the proximal 1.7 kb of the control region of the Drosophila melanogaster gene even skipped (eve). PMID: 16980977
    19. Eve is sufficient to regulate both voltage- and ligand-gated currents in motoneurons, extending its known repertoire of action beyond its already characterized role in axon guidance. PMID: 17147779
    20. Spatial specificity of mesodermal eve expression relies on multiple repressor sites. PMID: 18067885
    21. The sepsid and Drosophila enhancers drive nearly identical expression patterns in transgenic D. melanogaster embryos. PMID: 18584029
    22. the single ortholog of Dbx1/2 contributes to the development of specific subsets of interneurons via cross-repressive, lineage-specific interactions with the motoneuron-promoting factors eve and hb9 (exex) PMID: 19710170

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  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    Even-skipped homeobox family
  • 數據庫鏈接：

    KEGG: dme:Dmel_CG2328

    STRING: 7227.FBpp0087478

    UniGene: Dm.18510



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