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  4. Recombinant Drosophila melanogaster RuvB-like helicase 2 (rept)

Recombinant Drosophila melanogaster RuvB-like helicase 2 (rept)

  • 中文名稱：
    黑腹果蠅rept重組蛋白
  • 貨號：
    CSB-YP894638DLU
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅rept重組蛋白
  • 貨號：
    CSB-EP894638DLU
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅rept重組蛋白
  • 貨號：
    CSB-EP894638DLU-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅rept重組蛋白
  • 貨號：
    CSB-BP894638DLU
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅rept重組蛋白
  • 貨號：
    CSB-MP894638DLU
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    rept
  • Uniprot No.：
  • 別名：
    rept; CG9750; RuvB-like helicase 2; EC 3.6.4.12; Dreptin; Drep; Reptin
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-481
  • 氨基酸序列
    MAETEKIEVR DVTRIERIGA HSHIRGLGLD DVLEARLVSQ GMVGQKDARR AAGVVVQMVR EGKIAGRCIL LAGEPSTGKT AIAVGMAQAL GTETPFTSMS GSEIYSLEMS KTEALSQALR KSIGVRIKEE TEIIEGEVVE IQIERPASGT GQKVGKVTLK TTEMETNYDL GNKIIECFMK EKIQAGDVIT IDKASGKVNK LGRSFTRARD YDATGAQTRF VQCPEGELQK RKEVVHTVTL HEIDVINSRT HGFLALFSGD TGEIKQEVRD QINNKVLEWR EEGKAEINPG VLFIDEVHML DIECFSFLNR ALESDMAPVV VMATNRGITR IRGTNYRSPH GIPIDLLDRM IIIRTVPYSE KEVKEILKIR CEEEDCIMHP DALTILTRIA TDTSLRYAIQ LITTANLVCR RRKATEVNTE DVKKVYSLFL DENRSSKILK EYQDDYMFSE ITEEVERDPA AGGGAKRRVE GGGGDAQPME H
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Acts as a transcriptional coactivator in Wg signaling caused by altered arm signaling. Pont and rept interfere antagonistically with nuclear arm signaling function, and are required to enhance or reduce arm activity, respectively. Also an essential cofactor for the normal function of Myc; required for cellular proliferation and growth.; Proposed core component of the chromatin remodeling Ino80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair.
  • 基因功能參考文獻：
    1. Rept and pontin have conserved their ability to bind to Myc during evolution PMID: 16087886
    2. Drosophila Reptin participates in epigenetic processes leading to a repressive chromatin state. PMID: 16816423
    3. The enzymatic functions of Rept and Pont are indispensable for maintaining Hox gene expression states, highlighting the importance of these two antagonistic factors in transcriptional output. PMID: 18259215
  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    RuvB family
  • 組織特異性：
    Higher expression occurs in primordia of mesoderm, anterior and posterior midgut and cephalic furrow early in gastrulation, as well as in endoderm and mesoderm lineages during germ band extension. Later in development expression is only maintained in endo
  • 數據庫鏈接：

    KEGG: dme:Dmel_CG9750

    STRING: 7227.FBpp0074756

    UniGene: Dm.7319



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