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Recombinant Drosophila melanogaster Ras-like protein 1 (Ras85D)

  • 中文名稱:
    黑腹果蠅Ras85D重組蛋白
  • 貨號:
    CSB-YP362473DLU
  • 規格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    黑腹果蠅Ras85D重組蛋白
  • 貨號:
    CSB-EP362473DLU
  • 規格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    黑腹果蠅Ras85D重組蛋白
  • 貨號:
    CSB-EP362473DLU-B
  • 規格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    黑腹果蠅Ras85D重組蛋白
  • 貨號:
    CSB-BP362473DLU
  • 規格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    黑腹果蠅Ras85D重組蛋白
  • 貨號:
    CSB-MP362473DLU
  • 規格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    Ras85D
  • Uniprot No.:
  • 別名:
    Ras85D; Ras1; CG9375; Ras-like protein 1; Dras1; Dmras85D
  • 種屬:
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度:
    full length protein
  • 表達區域:
    1-186
  • 氨基酸序列
    MTEYKLVVVG AGGVGKSALT IQLIQNHFVD EYDPTIEDSY RKQVVIDGET CLLDILDTAG QEEYSAMRDQ YMRTGEGFLL VFAVNSAKSF EDIGTYREQI KRVKDAEEVP MVLVGNKCDL ASWNVNNEQA REVAKQYGIP YIETSAKTRM GVDDAFYTLV REIRKDKDNK GRRGRKMNKP NRRFKC
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays a role in eye development by regulating cell growth, survival of postmitotic ommatidial cells and differentiation of photoreceptor cells. During larval development, mediates Ptth/tor signaling leading to the production of ecdysone, a hormone required for the initiation of metamorphosis.
  • 基因功能參考文獻:
    1. In conclusion, Ras(V12)-modified caspases initiate a feedback amplification loop involving tumorous epithelial cells and macrophage-like immune cells that is necessary for uncontrolled tumor growth and invasive behavior. PMID: 28853394
    2. Thus, our current work provides a novel mechanism of pupal size determination by microRNAs during Drosophila melanogaster metamorphosis. PMID: 27733364
    3. Oncogenic Ras stimulates Eiger/TNF exocytosis to promote growth. PMID: 25411211
    4. Inhibition of Ras1 signaling leads to reduced Appl expression, whereas constitutive activation drives ectopic Appl expression. PMID: 23178937
    5. Data show that the ras1(R68Q) mutant is homozygous viable but causes various developmental defects associated with elevated Ras signaling. PMID: 21858158
    6. Results conclude that the membrane association of Drosophila Ras1 is not required for eye development. PMID: 20937772
    7. interaction between Ras(V12) and scrib(-) clones involves JNK signalling propagation and JNK-induced upregulation of JAK/STAT-activating cytokines, a compensatory growth mechanism for tissue homeostasis PMID: 20072127
    8. In the Drosophila eye, Ras may protect cells from apoptosis by inhibiting both JNK and Hid activities. Surprisingly, reducing Ras activity in the wing, however, does not cause apoptosis but rather affects cell and organ size. PMID: 19840402
    9. Ras activity in the prothoracic gland regulates body size and the duration of each larval stage by regulating ecdysone release. PMID: 16182526

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  • 亞細胞定位:
    Cell membrane; Lipid-anchor; Cytoplasmic side. Note=Loss of prenylation causes protein location to the cytoplasm.
  • 蛋白家族:
    Small GTPase superfamily, Ras family
  • 組織特異性:
    Expressed in the posterior termini of the embryo, restricted mainly to the embryonic central nervous system, and in the eye imaginal disk.
  • 數據庫鏈接:

    KEGG: dme:Dmel_CG9375

    STRING: 7227.FBpp0081600

    UniGene: Dm.4812



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