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  4. Recombinant Drosophila melanogaster Protein yorkie (yki)

Recombinant Drosophila melanogaster Protein yorkie (yki)

  • 中文名稱：
    黑腹果蠅yki重組蛋白
  • 貨號：
    CSB-YP674941DLU
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅yki重組蛋白
  • 貨號：
    CSB-EP674941DLU
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅yki重組蛋白
  • 貨號：
    CSB-EP674941DLU-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅yki重組蛋白
  • 貨號：
    CSB-BP674941DLU
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅yki重組蛋白
  • 貨號：
    CSB-MP674941DLU
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    yki
  • Uniprot No.：
  • 別名：
    yki; CG4005; Transcriptional coactivator yorkie; Protein yorkie; Transcriptional coactivator YAP1 homolog
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-418
  • 氨基酸序列
    MCACLIAKII LCSFRLYTIS AFYMLTTMSA SSNTNSLIEK EIDDEDMLSP IKSNNLVVRV NQDTDDNLQA LFDSVLNPGD AKRPLQLPLR MRKLPNSFFT PPAPSHSRAN SADSTYDAGS QSSINIGNKA SIVQQPDGQS PIAAIPQLQI QPSPQHSRLA IHHSRARSSP ASLQQNYNVR ARSDAAAANN PNANPSSQQQ PAGPTFPENS AQEFPSGAPA SSAIDLDAMN TCMSQDIPMS MQTVHKKQRS YDVISPIQLN RQLGALPPGW EQAKTNDGQI YYLNHTTKST QWEDPRIQYR QQQQILMAER IKQNDVLQTT KQTTTSTIAN NLGPLPDGWE QAVTESGDLY FINHIDRTTS WNDPRMQSGL SVLDCPDNLV SSLQIEDNLC SNLFNDAQAI VNPPSSHKPD DLEWYKIN
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Transcriptional coactivator which is the critical downstream regulatory target in the Hippo/SWH (Sav/Wts/Hpo) signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein Hippo (Hpo), in complex with its regulatory protein Salvador (Sav), phosphorylates and activates Warts (Wts) in complex with its regulatory protein Mats, which in turn phosphorylates and inactivates the Yorkie (Yki) oncoprotein. The Hippo/SWH signaling pathway inhibits the activity of the transcriptional complex formed by Scalloped (sd) and Yki and the target genes of this pathway include cyclin-E (cycE), diap1 and bantam. Regulates the expression of G1/S-specific CycE and diap1, thereby promoting cell proliferation and inhibiting apoptosis. Required for transcriptional activity of sd in wing imaginal disks. Induces expression of expression of vestigial (vg) in wing and haltere disks and the expression of transcription factor E2f (E2f).
  • 基因功能參考文獻：
    1. Yorkie functions in a feedforward "amplifier" loop that promotes myosin activation PMID: 30032991
    2. Wts mediates effects of insulin and the neuropeptide prothoracicotropic hormone on regulation of ecdysone production through Yorkie and the microRNA bantam. Thus coupling insulin signaling with ecdysone production to adjust systemic growth in response to nutritional conditions during development. PMID: 28528906
    3. miR-8 modulates a positive growth regulator, Yorkie, and a negative growth regulator, brinker Deregulation of this network can result in the loss of tissue homeostasis and the formation of tumors. PMID: 29945869
    4. BAP complex depletion causes upregulation of the Wingless (Wg) and Decapentaplegic (Dpp) morphogens to promote tumor formation in cooperation with Yki. PMID: 28754838
    5. These results support a model in which elevated Yki signaling increases the number of hemocytes, which become melanotic tumors as a result of elevated JAK/STAT signaling. PMID: 28620086
    6. This study presents successfully overexpressed full length recombinant Tgi and Yki, experimentally confirms that intrinsic structural disorder is common to both proteins, and assesses binding of the Yki WW domains to the three Tgi PPxY motifs using nuclear magnetic resonance and isothermal titration calorimetry. PMID: 29257682
    7. miR-285 directly targets the Yki cofactor Mask to suppress Yki activity and down-regulates the expression of its downstream target cyclin E, a key regulator of cell cycle. PMID: 28265104
    8. In ovary escort cells (ECs) both Hh and Yki limit production of BMP ligands to allow germline differentiation. Loss of Yki produced a more severe germarial phenotype than loss of Hh signaling and principally induced a different BMP ligand. Moreover, Yki activity reporters and epistasis tests showed that Yki does not mediate the key actions of Hh signaling in ECs. PMID: 28619819
    9. We demonstrate the efficacy of the MARGE technique by flip-out (clonal and disc-wide) of a Ubi-GFP-carrying construct in imaginal discs, and by inducing a known yki mutant phenotype in the Drosophila ovary PMID: 27696669
    10. This study identified a potential nuclear localization signal at the N-terminal end of Yorkie as well as a critical role for Importin alpha 1 in Yorkie nuclear import. PMID: 26887950
    11. Data suggest that Tor (target of rapamycin protein) signaling governs Yki action after reaching nucleus by allowing access to target genes via Hpo (Hippo protein) pathway; Tor also promotes wing growth by liberating Yki from nuclear seclusion. PMID: 26474042
    12. Yki directly associates with NcoA6. PMID: 25017066
    13. Yki activity was cell-autonomously decreased but non-cell-autonomously elevated in tissues where the adherens junctions components E-cadherin or alpha-catenin were knocked down during imaginal disc development. PMID: 25624491
    14. epithelial tube size regulation defines new non-apoptotic roles for Yki, DIAP1 and Ice PMID: 25036253
    15. the Misshapen-Warts-Yorkie pathway acts in enteroblasts to control niche signaling to intestinal stem cells PMID: 25453828
    16. Drosophila casein kinase 2 (CK2) promotes warts protein to suppress Yorkie protein activity for growth control. PMID: 25320084
    17. Yorkie Controls Cell Fusion and Polyploidization in wound healing. PMID: 24184101
    18. This study identifies a novel protein Sd-Binding-Protein (SdBP)/Tgi, which directly competes with Yki for binding to Sd through its TDU domains and inhibits the Sd-Yki transcriptional activity. PMID: 23999857
    19. The association of Yki with GAF and BRM establishes a direct connection between Yki and chromatin-remodeling complexes as a mechanism by which Yki promotes transcription. PMID: 23395637
    20. Mask is a novel cofactor for Yki/YAP required for optimal Yki/YAP activity during development and oncogenesis. PMID: 23333314
    21. Mask forms a complex with Yki and its binding partner, Scalloped (Sd), on target-gene promoters and is essential for Yki to drive transcription of target genes and tissue growth. PMID: 23333315
    22. Yki activity is elevated among wild type cells. PMID: 22705500
    23. Data show that the non-cell autonomous induction of DIAP-1 is mediated by Yorkie, the conserved downstream effector of Hippo signaling. PMID: 22679484
    24. crosstalk between the insulin/IGF signaling pathway and Yorkie leads to coordinated regulation of these two pathways PMID: 22609549
    25. Yorkie promotes expression of the microRNA gene bantam in glia, and bantam promotes expression of Myc, which is required for Yorkie and bantam-induced glial proliferation. PMID: 22069188
    26. The data establish Myopic as a Yorkie regulator and implicate Myopic-dependent association of Yorkie with endosomal compartments as a regulatory step in nuclear outputs of the SWH pathway. PMID: 21571226
    27. show that the Hippo pathway components Warts and Yorkie mediate a transition from low- to high-level ISC proliferation to facilitate regeneration. The Hippo pathway regulates growth in diverse organisms and has been linked to cancer PMID: 20727758
    28. Activation of Yki in intestinal stem cells is sufficient to increase ISC proliferation, a process involving Yki target genes that promote division, survival and the Upd cytokines. PMID: 21098564
    29. Here we identify Yorkie (Yki), the Drosophila ortholog of the mammalian transcriptional coactivator yes-associated protein (YAP), as a missing link between Wts and transcriptional regulation. PMID: 16096061
    30. Warts inhibits Yki nuclear localization in vivo, and can promote binding of Yki to 14-3-3 proteins in cultured cells. PMID: 18256197
    31. Results show that although Ser168 is the most critical site, all three phosphorylation sites influence Yki localization and activity in vivo, and can be sites of regulation by Wts. PMID: 19330023
    32. Hth and Yki are bound to a DNA sequence approximately 14 kb upstream of the bantam hairpin in eye imaginal disc cells PMID: 19762509
    33. Genetic interaction between the Drosophila YAP orthologue Yorkie and Egfr signalling components supports the link between these two highly conserved signalling pathways. PMID: 19935651

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  • 亞細胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    YAP1 family
  • 數據庫鏈接：

    KEGG: dme:Dmel_CG4005

    STRING: 7227.FBpp0288697

    UniGene: Dm.4183



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